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Screening of expression libraries using ELISA : identification of immunogenic p...

Kalapothakis, Evanguedes; Jardim, S.; Magalhães, Ana Cristina Ribeiro; Mendes, Thais Melo; Marco, Luiz de; Afonso, Luís Carlos Crocco

The present report describes the use of ELISA with cDNA expression libraries in the identification of immunogenic proteins. The methodology described was applied using libraries constructed with mRNA isolated from Tityus serrulatus and Tityus bahiensis venom glands. In addition we describe for the first time the sequence of a neurotoxin from Tityus bahiensis venom gland named TbTx5 whose amino acid sequencing s...

Date: 2015   |   Origin: Oasisbr
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