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Genome-wide identification, phylogeny, and gene duplication of the epigenetic r...

Alves, Sofia; Braga, Ângelo; Parreira, Denise; Alhinho, Ana Teresa; Silva, Helena Sofia Gomes; Ramos, Miguel Jesus Nunes; Costa, Maria Manuela Ribeiro

Epigenetic regulators are proteins involved in controlling gene expression. Information about the epigenetic regulators within the Fagaceae, a relevant family of trees and shrubs of the northern hemisphere ecosystems, is scarce. With the intent to characterize these proteins in Fagaceae, we searched for orthologs of DNA methyltransferases (DNMTs) and demethylases (DDMEs) and Histone modifiers involved in acetyl...


The dynamics of flower development in Castanea sativa Mill

Alhinho, Ana Teresa Talhinhas Barata; Ramos, Miguel Jesus Nunes; Alves, Sofia; Rocheta, Margarida; Morais-Cecílio, Leonor; Gomes-Laranjo, José

The sweet chestnut tree (<i>Castanea sativa</i> Mill.) is one of the most significant Mediterranean tree species, being an important natural resource for the wood and fruit industries. It is a monoecious species, presenting unisexual male catkins and bisexual catkins, with the latter having distinct male and female flowers. Despite the importance of the sweet chestnut tree, little is known regarding the molecul...


Genome-wide identification of epigenetic regulators in Quercus suber L.

Silva, Helena G.; Sobral, Rómulo Sacramento; Magalhães, Alexandre P.; Morais-Cecílio, Leonor; Costa, Maria Manuela Ribeiro

Modifications of DNA and histones, including methylation and acetylation, are critical for the epigenetic regulation of gene expression during plant development, particularly during environmental adaptation processes. However, information on the enzymes catalyzing all these modifications in trees, such as <i>Quercus suber</i> L., is still not available. In this study, eight DNA methyltransferases (DNA Mtases) a...


Advancing Eucalyptus Genomics: Cytogenomics Reveals Conservation of Eucalyptus ...

Ribeiro, Teresa; Barrela, Ricardo M.; Bergès, Hélène; Marques, Cristina; Loureiro, João; Morais-Cecílio, Leonor; Paiva, Jorge A. P.

The genus Eucalyptus encloses several species with high ecological and economic value, being the subgenus Symphyomyrtus one of the most important. Species such as E. grandis and E. globulus are well characterized at the molecular level but knowledge regarding genome and chromosome organization is very scarce. Here we characterized and compared the karyotypes of three economically important species, E. grandis, ...


The quest for molecular regulation underlying unisexual flower development

Sobral, Rómulo Sacramento; Silva, Helena G; Morais-Cecílio, Leonor; Costa, Maria Manuela Ribeiro

The understanding of the molecular mechanisms responsible for the making of a unisexual flower has been a long-standing quest in plant biology. Plants with male and female flowers can be divided mainly into two categories: dioecious and monoecious, and both sexual systems co-exist in nature in ca of 10% of the angiosperms. The establishment of male and female traits has been extensively described in a hermaphro...


Comparative transcriptomic analysis of male and female flowers of monoecious Qu...

Rocheta, Margarida; Sobral, Rómulo Sacramento; Magalhães, Joana; Amorim, Maria I.; Ribeiro, Teresa; Pinheiro, Miguel; Egas, Conceição

Monoecious species provide a comprehensive system to study the developmental programs underlying the establishment of female and male organs in unisexual flowers. However, molecular resources for most monoecious non-model species are limited, hampering our ability to study the molecular mechanisms involved in flower development of these species. The objective of this study was to identify differentially express...


A comprehensive assessment of the transcriptome of cork oak (Quercus suber) thr...

Pereira-Leal, José B; Abreu, Isabel A; Alabaça, Cláudia S; Almeida, Maria; Almeida, Paulo; Almeida, Tânia; Amorim, Maria; Araújo, Susana

Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic information. Cork ...


A comprehensive assessment of the transcriptome of cork oak (Quercus suber) thr...

Pereira-Leal, José B; Abreu, Isabel A; Alabaça, Cláudia S; Almeida, Maria Helena; Almeida, Paulo; Almeida, Tânia; Amorim, Maria Isabel; Araújo, Susana

Background: Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic inform...


A comprehensive assessment of the transcriptome of cork oak (Quercus suber) thr...

Pereira-Leal, José B.; Abreu, Isabel A.; Alabaça, Cláudia S.; Almeida, Maria H.; Almeida, Paulo; Almeida, Tânia; Amorim, Maria I.; Araújo, Susana

Background: Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic inform...


Genomic organization and dynamics of repetitive DNA sequences in representative...

Alves, Sofia; Ribeiro, Teresa; Inácio, Vera; Rocheta, Margarida; Morais-Cecílio, Leonor

Oaks, chestnuts, and beeches are economically important species of the Fagaceae. To understand the relationship between these members of this family, a deep knowledge of their genome composition and organization is needed. In this work, we have isolated and characterized several AFLP fragments obtained from Quercus rotundifolia Lam. through homology searches in available databases. Genomic polymorphisms involvi...


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