11 documents found, page 1 of 2
The Dystrophin-Dystroglycan complex ensures cytokinesis efficiency in Drosophil...
Gonçalves, M; Lopes, C; Alégot, H; Osswald, M; Bosveld, F; Ramos, C; Richard, G; Bellaiche, Y; Mirouse, V; Morais-de-Sá, ECytokinesis physically separates daughter cells at the end of cell division. This step is particularly challenging for epithelial cells, which are connected to their neighbors and to the extracellular matrix by transmembrane protein complexes. To systematically evaluate the impact of the cell adhesion machinery on epithelial cytokinesis efficiency, we performed an RNAi-based modifier screen in the Drosophila fo...
Systematic analysis of RhoGEF/GAP localizations uncovers regulators of mechanos...
Pietro, F; Osswald, M; Heras, JM; Cristo, I; López-Gay, J; Wang, Z; Pelletier, S; Gaugué, I; Leroy, A; Martin, C; Morais-de-Sá, E; Bellaïche YCell proliferation is central to epithelial tissue development, repair, and homeostasis. During cell division, small RhoGTPases control both actomyosin dynamics and cell-cell junction remodeling to faithfully segregate the genome while maintaining tissue polarity and integrity. To decipher the mechanisms of RhoGTPase spatiotemporal regulation during epithelial cell division, we generated a transgenic fluorescen...
Balancing cell polarity PARts through dephosphorylation
Barros-Carvalho, A; Morais-de-Sá, EHow cells spatially organize their plasma membrane, cytoskeleton, and cytoplasm remains a central question for cell biologists. In this issue of JCB, Calvi et al. (2022. J. Cell Biol. https://doi.org/10.1083/jcb.202201048) identify PP1 phosphatases as key regulators of C. elegans anterior–posterior polarity, by counterbalancing aPKC-mediated phosphorylation of PAR-2.
aPKC regulates apical constriction to prevent tissue rupture in the Drosophila ...
Osswald, M; Barros-Carvalho, A; Carmo, AM; Loyer, N; Gracio, PC; Sunkel, CE; Homem, CCF; Januschke, J; Morais-de-Sá, EApical-basal polarity is an essential epithelial trait controlled by the evolutionarily conserved PAR-aPKC polarity network. Dysregulation of polarity proteins disrupts tissue organization during development and in disease, but the underlying mechanisms are unclear due to the broad implications of polarity loss. Here, we uncover how Drosophila aPKC maintains epithelial architecture by directly observing tissue ...
Germline cdh1 g212e missense variant: Combining clinical, in vitro and in vivo ...
Figueiredo, J; Mercadillo, F; Melo, S; Barroso, A; Gonçalves, M; Díaz-Tasende, J; Carneiro, P; Robles, L; Colina, F; Ibarrola, C; Perea, JE-cadherin, encoded by CDH1, is an essential molecule for epithelial homeostasis, whose loss or aberrant expression results in disturbed cell–cell adhesion, increased cell invasion and metas-tasis. Carriers of CDH1 germline mutations have a high risk of developing diffuse gastric cancer and lobular breast cancer, associated with the cancer syndrome Hereditary Diffuse Gastric Cancer (HDGC). The ubiquitous availa...
Spatiotemporal control of mitotic exit during anaphase by an aurora B-Cdk1 cros...
Afonso, O; Castellani, CM; Cheeseman, LP; Ferreira, JG; Orr, B; Ferreira, LT; Chambers, JJ; Morais-de-Sá, E; Maresca, TJ; Maiato, HAccording to the prevailing ‘clock’ model, chromosome decondensation and nuclear envelope reformation when cells exit mitosis are byproducts of Cdk1 inactivation at the metaphase-anaphase transition, controlled by the spindle assembly checkpoint. However, mitotic exit was recently shown to be a function of chromosome separation during anaphase, assisted by a midzone Aurora B phosphorylation gradient-the ‘ruler’...
Light-induced protein clustering for optogenetic interference and protein inter...
Osswald, M; Santos, AF; Morais-de-Sá, EDrosophila Schneider 2 (S2) cells are a simple and powerful system commonly used in cell biology because they are well suited for high resolution microscopy and RNAi-mediated depletion. However, understanding dynamic processes, such as cell division, also requires methodology to interfere with protein function with high spatiotemporal control. In this research study, we report the adaptation of an optogenetic t...
PP1-Mediated Dephosphorylation of Lgl Controls Apical-basal Polarity
Moreira, S; Osswald, M; Ventura, G; Gonçalves, M; Sunkel, CE; Morais-de-Sá, EApical-basal polarity is a common trait that underlies epithelial function. Although the asymmetric distribution of cortical polarity proteins works in a functioning equilibrium, it also retains plasticity to accommodate cell division, during which the basolateral determinant Lgl is released from the cortex. Here, we investigated how Lgl restores its cortical localization to maintain the integrity of dividing e...
Emerging Mechanisms and Roles for Asymmetric Cytokinesis
Thieleke-Matos, C; Osório, DS; Carvalho, AX; Morais-de-Sá, ECytokinesis completes cell division by physically separating the contents of the mother cell between the two daughter cells. This event requires the highly coordinated reorganization of the cytoskeleton within a precise window of time to ensure faithful genomic segregation. In addition, recent progress in the field highlighted the importance of cytokinesis in providing particularly important cues in the context...
Spatiotemporal phosphoregulation of Lgl: Finding meaning in multiple on/off but...
Moreira, S; Morais-de-Sá, EIntracellular asymmetries, often termed cell polarity, determine how cells organize and divide to ultimately control cell fate and shape animal tissues. The tumor suppressor Lethal giant larvae (Lgl) functions at the core of the evolutionarily conserved cell polarity machinery that controls apico-basal polarization. This function relies on its restricted basolateral localization via phosphorylation by aPKC. Her...