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Detection of Rickettsia in ticks using loop-mediated isothermal amplification (...

Lansdell, Samantha; Hassan, Marwa M.; La Ragione, Roberto; Betson, Martha; Nuncio, MS; Lopes de Carvalho, Isabel; Zé-Zé, Líbia; de Sousa, Rita

Objectives: The objective of the study was to develop a Loop-mediated Isothermal Amplification (LAMP) assay for screening of Rickettsia species circulating in ticks using the citrate synthase gene (gltA). The LAMP assay employed portable visualisation methods, making the assay more field-suitable. Furthermore, prior methods have not used gltA as the target, despite proven success in Polymerase Chain Reaction (P...


Communicating and disseminating One Health: successes of the One Health Europea...

Taylor, Emma; Artursson, Karin; Busani, Luca; Callegari, Arnaud; Cantlay, Jennifer; Caniça, Manuela; Campling, Elaine; Gavier-Widén, Dolores

The application of a One Health approach recognizes that human health, animal health, plant health and ecosystem health are intrinsically connected. Tackling complex challenges associated with foodborne zoonoses, antimicrobial resistance, and emerging threats is imperative. Therefore, the One Health European Joint Programme was established within the European Union research programme Horizon 2020. The One Healt...


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