64 documents found, page 1 of 7
Cellulose-based colorimetric test strips for SARS-CoV-2 antibody detection
Sousa, Mariana; Pereira, Ana Cláudia; Correia, Bárbara; Carmo, Anália do; Matos, Ana Miguel; Cruz, Maria Teresa; Moreira, FelisminaThe COVID-19 pandemic highlighted the need for rapid, cost-effective tools to monitor transmission and immune response. We developed two novel paper-based colorimetric biosensors using glutaraldehyde as a protein dyeits first use in this context. Glutaraldehyde reacts with amino groups to generate a brown color, enabling detection of SARS-CoV-2 antibodies. Wathman filter paper was functionalized with (3-aminopr...
Cellulose test strips modified with virus-like particles: Advancing viral immun...
Pereira, Ana Cláudia Santos; Sousa, Cristina; Mateus, Daniela; Sebastião, Ana Isabel; Cruz, Maria Teresa; Mato, Ana Miguel; Moreira, FelisminaThe COVID-19 pandemic has driven immunity acquisition through infection or vaccination, leading to SARS-CoV-2 antibodies. This study introduces a colorimetric biosensor for COVID-19 immunity screening using Virus-Like Particles (VLPs) with SARS-CoV-2 spike protein motifs on a cellulose carrier. The cellulose was chemically modified with DSS to secure the VLPs, verified via FTIR. Detection utilized the Bradford ...
An Innovative Approach for Tailoring Molecularly Imprinted Polymers for Biosens...
Oliveira, Daniela dos Santos; Oliveira, Andreia Sofia Rodrigues; Mendonça, Patrícia Vitorino; Coelho, Jorge Fernando JordãoThis work presents a novel approach for tailoring molecularly imprinted polymers (MIPs) with a preliminary stage of atom transfer radical polymerization (ATRP), for a more precise definition of the imprinted cavity. A well-defined copolymer of acrylamide and N,N′-methylenebisacrylamide (PAAm-co-PMBAm) was synthesized by ATRP and applied to gold electrodes with the template, followed by a crosslinking reaction. ...
An electrochemically synthesized molecularly imprinted polymer for highly selec...
Oliveira, Daniela; Romaguera Barcelay, Yonny; Moreira, FelisminaIn this study, a molecularly imprinted polymer film (MIP) was prepared on the surface of a disposable carbon screen-printed electrode (C-SPE) using (3-acrylamidopropyl)trimethylammonium chloride (AMPTMA) as a functional monomer and the cancer biomarker carbohydrate antigen 15-3 (CA 15-3) as a template. The MIP was synthesized by in situ electropolymerization (ELP) of the AMPTMA monomer in the presence of the CA...
Bioinspired host-tailored polymers based on molecular imprinting for cytokine a...
Ferreira, Bianca; Correa-Duarte, Miguel; Marques, Arcelina; Moreira, Felismina; Martins, GabrielaMolecular imprinting undergone a substantial boost driven by the awareness of molecularly imprinted polymers (MIPs)-ligand recognition skills. In particular, the introduction of natural-based compounds like cyclodextrins into the structural scaffold of synthetic recognition elements attracted great importance as a novel route to design more friendly-environments for protein binding, while promoting higher selec...
Colorimetric cellulose-based test strip for the rapid assessment of pathogens e...
Vlad, Anitei; Moreira, Felismina; Guerreiro, Joana(Introduction) Antibody testing plays a crucial role in identifying individuals previously infected with a specific pathogen. The role of antibody testing gained extreme importance, especially during the COVID-19 pandemic, as monitoring anti-SARS-CoV-2 antibodies provides vital epidemiological and clinical insights into the evolution of coronavirus disease and the stratification of immunized and asymptomatic po...
Alzheimer's diagnosis beyond cerebrospinal fluid: Probe-Free Detection of Tau P...
Arjum, Ajith Mohan; Deshpande, Sudhaunsh; Dunlop, Tom; Norman, Beth; Oliviera, Daniela; Vulpe, Georgeta; Moreira, Felismina; Sharma, SanjivPhosphorylated Tau proteins are promising biomarkers for the diagnosis and prognosis of Alzheimer's disease. This study presents a novel voltametric sensor using a vanadium MXene polydopamine (VxPDA) redox active composite and a Tau-441-specific polyaniline molecularly imprinted polymer (PANI MIP) for the sensitive detection of Tau-441 in interstitial fluid (ISF) and plasma. The VxPDA/PANI MIP sensor demonstrat...
Molecularly imprinting for ca 15-3 protein detection: a promising biosensor for...
Oliveira, Daniela; Barcelay, Yonny Romaguera; Moreira, Felismina(Introduction) Cancer is a public health problem worldwide. Early detection is crucial to improve survival rates [1]. Non-invasive, fast rapid, and low-cost diagnostic methods are urgently needed. Biosensors are a promising alternative to traditional methods such as ELISA, enabling point-ofcare (PoC) analyses. These require biorecognition elements with high selectivity and stability to meet current requirements...
Paper-based biomimetic test-strip for CA15-3 with coloured readout
Carneiro, Mariana C. C. G.; Rodrigues, Lígia R.; Moreira, Felismina; Sales, M. Goreti F.The combination of molecularly-imprinted polymers (MIPs) on paper substrates for capturing a cancer antigen 15–3 (CA15-3) with the traditional coloured transduction scheme of enzyme-linked immunosorbent assay (ELISA) using 3,3′,5,5′-tetramethylbenzidine (TMB), horseradish peroxidase (HRP) and H2O2 is presented here for the first time. Here, the paper surface was modified with a silane derivative containing an a...
Liquid Redox Probe-Free Plastic Antibody Development for Malaria Biomarker Reco...
Glória, Juliane Corrêa; Oliveira, Daniela S.; Gandarilla, Ariamna Dip; Barcelay, Yonny Romaguera; Mariúba, Luis André Morais; Nogueira, Paulo AfonsoMalaria is a major public health challenge worldwide and requires accurate and efficient diagnostic methods. Traditional diagnostic approaches based on antigen–antibody interactions are associated with ethical and economic concerns. Molecularly imprinted polymers (MIPs) offer a promising alternative by providing a complementary polymer structure capable of selectively binding target molecules. In this study, we...