6 documents found, page 1 of 1
Exploring cannabidiol (CBD) and cannabigerol (CBG) safety profile and skincare ...
Luz-Veiga, Mariana; Mendes, Adélia; Tavares-Valente, Diana; Amorim, Manuela; Conde, António; Pintado, Manuela Estevez; Moreira, Helena R.Cannabinoids have long been known for their bioactive properties, with their topical application as anti-inflammatory compounds being at the forefront of research for the past decade. Concurrently, the cosmetic market is a fast-growing industry in constant need of new biomolecules. In this work, we studied the safety profile for topical applications of two cannabinoids: cannabidiol (CBD) and cannabigerol (CBG) ...
Pre-selection of fibroblasts subsets prompt prevascularization of tissue engine...
Moreira, Helena R.; Cerqueira, Mariana T.; Silva, Lucília Pereira; Pires, Joana; Jarnalo, Mariana; Horta, Ricardo; Reis, R. L.; Marques, A. P.The papillary and reticular dermis harbors phenotypically distinct fibroblasts, whose functions such as maintenance of skin's microvasculature are also distinct. Thus, we hypothesized that pre-selection of the subpopulations of fibroblasts would benefit the generation of skin tissue engineered (TE) constructs, promoting their prevascularization in vitro. We first isolated papillary and reticular fibroblasts usi...
Spongy-like hydrogels prevascularization with the adipose tissue vascular fract...
Moreira, Helena R.; Rodrigues, Daniel Barreira; Ribeiro, Sara Freitas; Silva, Lucília Pereira; Morais, Alain da S.; Jarnalo, Mariana; Horta, RicardoIn vitro prevascularization is one of the most explored approaches to foster engineered tissue vascularization. We previously demonstrated a benefit in tissue neovascularization by using integrin-specific biomaterials prevascularized by stromal vascular fraction (SVF) cells, which triggered vasculogenesis in the absence of extrinsic growth factors. SVF cells are also associated to biological processes important...
Highly tailorable gellan gum nanoparticles as a platform for the development of...
Rodrigues, Daniel Barreira; Moreira, Helena R.; Cerqueira, Mariana T.; Marques, A. P.; Castro, António G.; Reis, R. L.; Pirraco, Rogério P.Background: T cell priming has been shown to be a powerful immunotherapeutic approach for cancer treatment in terms of efcacy and relatively weak side efects. Systems that optimize the stimulation of T cells to improve therapeutic efcacy are therefore in constant demand. A way to achieve this is through artifcial antigen presenting cells that are complexes between vehicles and key molecules that target relevant...
Integrin-specific hydrogels for growth factor-free vasculogenesis
Moreira, Helena R.; Rodrigues, Daniel Barreira; Ribeiro, Sara Freitas; Silva, Lucília Pereira; Morais, Alain da S.; Jarnalo, Mariana; Horta, RicardoIntegrin-binding biomaterials have been extensively evaluated for their capacity to enable de novo formation of capillary-like structures/vessels, ultimately supporting neovascularization in vivo. Yet, the role of integrins as vascular initiators in engineered materials is still not well understood. Here, we show that αvβ3 integrin-specific 3D matrices were able to retain PECAM1+ cells from the stromal vascu...
Tailoring gellan gum spongy-like hydrogels’ microstructure by controlling freez...
Moreira, Helena R.; Silva, Lucília P. da; Reis, R. L.; Marques, Alexandra P.Gellan gum (GG) spongy-like hydrogels have been explored for different tissue engineering (TE) applications owing to their highly attractive hydrogel-like features, and improved mechanical resilience and cell performance. Although the whole process for the preparation of these materials is well-defined, we hypothesized that variations occurring during the freezing step lead to batch-to-batch discrepancies. Aimi...