6 documents found, page 1 of 1
Diversity and distribution of microbial communities on the surface of External ...
Viegas, Cristina A.; Borsoi, Giovanni; Moreira, Leonilde M.; Parracha, João L.; Nunes, Lina; Malanho, Sofia; Veiga, Rosário; Flores-Colen, InêsExternal Thermal Insulation Composite Systems (ETICS) are frequently used to enhance the energy efficiency of the built environment. However, stains of presumable biological nature are often detected shortly after application, causing cladding defacement and altering the building aesthetics. To address which microbiota could contribute to these biodeterioration related color/aesthetic anomalies, samples collect...
Transcriptome profiling of human pluripotent stem cell‐derived cerebellar organ...
Silva, Teresa P.; Sousa-Luis, Rui; Fernandes, Tiago G.; Bekman, Evguenia; Rodrigues, Carlos A.V.; Vaz, Sandra H.; Moreira, Leonilde M.; Hashimura, YasHuman-induced pluripotent stem cells (iPSCs) have great potential for disease modeling. However, generating iPSC-derived models to study brain diseases remains a challenge. In particular, the ability to recapitulate cerebellar development in vitro is still limited. We presented a reproducible and scalable production of cerebellar organoids by using the novel single-use Vertical-Wheel bioreactors, in which funct...
Discussão de estratégias para a avaliação da biocolonização em ETICS : um caso ...
Borsoi, Giovanni; Parracha, João Luís; de Cooman, Quinten; Flores-Coles, Inês; Viegas, Cristina A.; Nunes, Lina; Moreira, Leonilde M.; Veiga, RosárioConservar e Reabilitar monumentos, edifícios e espaços são oportunidades únicas para fomentar a sustentabilidade dos territórios, constituindo-se o ENCORE 2020 - 4º Encontro de Conservação e Reabilitação de Edifícios numa ocasião ímpar para discutir os desafios da transformação no espaço construído: otimizar recursos, reutilizar materiais, melhorar a eficiência energética, repensar as centralidades urbanas, pro...
The regulator LdhR and the d-lactate dehydrogenase LdhA of Burkholderia multivo...
Silva, Inês N.; Ramires, Marcelo J.; Azevedo, Lisa A.; Guerreiro, Ana R.; Tavares, Andreia C.; Becker, Jörg D.; Moreira, Leonilde M.LysR-type transcriptional regulators (LTTR) are the most commonly found regulators in Burkholderia cepacia complex, comprising opportunistic pathogens causing chronic respiratory infections in cystic fibrosis (CF) patients. Despite LTTRs being global regulators of pathogenicity in several bacteria, few have been characterized in Burkholderia Here, we showed that gene ldhR of B. multivorans encoding a LTTR is co...
The Sinorhizobium meliloti EmrR Regulator Is Required for Efficient Colonizatio...
Santos, Mário R.; Marques, Andreia T.; Becker, Jörg D.; Moreira, Leonilde M.The nitrogen-fixing bacterium Sinorhizobium meliloti must adapt to diverse conditions encountered during its symbiosis with leguminous plants. We characterized a new symbiotically relevant gene, emrR (SMc03169), whose product belongs to the TetR family of repressors and is divergently transcribed from emrAB genes encoding a putative major facilitator superfamily-type efflux pump. An emrR deletion mutant produce...
Absence of functional TolC protein causes increased stress response gene expres...
Santos, Mario R.; Cosme, Ana M.; Becker, Jorg D.; Medeiros, Joao M C.; Mata, Marcia F.; Moreira, Leonilde M.The TolC protein from Sinorhizobium meliloti has previously been demonstrated to be required for establishing successful biological nitrogen fixation symbiosis with Medicago sativa. It is also needed in protein and exopolysaccharide secretion and for protection against osmotic and oxidative stresses. Here, the transcriptional profile of free-living S. meliloti 1021 tolC mutant is described as a step toward unde...