5 documents found, page 1 of 1
Mo-CBP3, an antifungal Chitin-Binding Protein from Moringa oleifera Seeds, is a...
Freire, José E. C.; Vasconcelos, Ilka M.; Moreno, Frederico B. M. B.; Batista, Adelina B.; Lobo, Marina D. P.; Pereira, Mirella L.; Lima, João P. M. S.Mo-CBP3 is a chitin-binding protein from M. oleifera seeds that inhibits the germination and mycelial growth of phytopathogenic fungi. This protein is highly thermostable and resistant to pH changes, and therefore may be useful in the development of new antifungal drugs. However, the relationship of MoCBP3 with the known families of carbohydrate-binding domains has not been established. In the present study, fu...
Crystal structure of an antifungal osmotin-like protein from Calotropis procera...
Ramos, Márcio Viana; Oliveira, Raquel S. B. de; Pereira, Humberto M.; Moreno, Frederico B. M. B.; Lobo, Marina D. P.; Rebelo, Luciana MagalhãesCpOsm is an antifungal osmotin/thaumatin-like protein purified from the latex of Calotropis procera. The protein is relatively thermostable and retains its antifungal activity over a wide pH range; therefore, it may be useful in the development of new antifungal drugs or transgenic crops with enhanced resistance to phytopathogenic fungi. To gain further insight into the mechanism of action of CpOsm, its three-d...
A trypsin inhibitor purified from Cassia leiandra seeds has insecticidal activi...
Dias, Lucas P.; Oliveira, Jose T. A.; Rocha-Bezerra, Lady C. B.; Sousa, Daniele O. B.; Costa, Helen P. S.; Araújo, Nadine M. S.; Carvalho, Ana F. U.A trypsin inhibitor from Cassia leiandra seeds, named ClTI, was purified, characterized, and its insecticidal activity against Aedes aegypti evaluated. ClTI was purified by DEAE-Cellulose and trypsin-Sepharose 4B chromatography, with a 15.5-fold purification and 2.4% yield. ClTI is composed of a 19,484 Da polypeptide chain as revealed by mass spectrometry, it is not a glycoprotein, its amino acid sequence is si...
A novel peroxidase purified from Marsdenia megalantha latex inhibits phytopatho...
Oliveira, Henrique P.; Silva, Rodolpho G. G; Oliveira, Jose T. A.; Sousa, Daniele O. B.; Pereira, Mirella L.; Souza, Pedro F. N.; Soares, Arlete A.An antifungal class III peroxidase was purified from Marsdenia megalantha latex (named Mo-POX) using DEAE-cellulose and gel filtration chromatography on a Superose 12 HR 10/30 column. Mm-POX has an apparent molecular mass of 67.0 kDa and a pI of 5.2, shares identity with other peroxidases, and fol lows Michaelis-Menten kinetics. It has a high affinity for guaiacol and hydrogen peroxide. The pH and temperature o...
Structure of a lectin from Canavalia gladiata seeds: new structural insights fo...
Delatorre, Plinio; Rocha, Bruno A. M.; Souza, Emmanuel P.; Oliveira, Taiana M.; Bezerra, Gustavo A.; Moreno, Frederico B. M. B.; Freitas, Beatriz T.Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2014-02-26T17:00:06Z No. of bitstreams: 1 WOS000249301900001.pdf: 1962990 bytes, checksum: 0c65e4a3836e97a4f347eb45bda09f6a (MD5); Made available in DSpace on 2014-02-26T17:00:07Z (GMT). No. of bitstreams: 1 WOS000249301900001.pdf: 1962990 bytes, checksum: 0c65e4a3836e97a4f347eb45bda09f6a (MD5) Previous issue date: 2007-08-02; Submitted by Vitor Si...