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Green versus conventional extraction of tunisian carob pulp: polyphenols, antio...

Saadi, Chaima; Sakouhi, Faouzi; Chami, Mohamad Ali El; Cáceres-Jiménez, Salud; Moreno-Ortega, Alicia; Boukhchina, Sadok; Moreno-Rojas, José Manuel

Carob pulp is a Mediterranean by-product highlighted as an important source of bioactive compounds potentially used in different industrial applications. This study compares extraction methods using both conventional (maceration-M, organic solvents and water) and non-conventional green techniques (ultrasound-assisted extraction -UAE, and natural deep eutectic solvents -NADES). Chemometric tools were applied to ...


Phytochemical characterization and bioactivity of extracts from different fruit...

Hamdoun, Ouafaa; Gonçalves, Sandra; Paixão Mansinhos, Inês Filipa; Rodríguez Solana, Raquel; Pereira-Caro, Gema; Moreno-Rojas, José Manuel

The objective of this study was to analyze the chemical profile of extracts from different fruit parts of the perennial arborescent cactus Opuntia leucotricha by applying ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) and to evaluate their biological properties (antioxidant and enzyme inhibitory capacities). Extracts were obtained from the fruit pulp (without or with...


Environmental factors related to climate change alter the chemical composition ...

Paixão Mansinhos, Inês Filipa; Gonçalves, Sandra; Rodríguez Solana, Raquel; Moreno-Rojas, José Manuel; Romano, Anabela

Climate change is affecting all regions of the world, and the Mediterranean region is one of the most affected. Plants accumulate secondary metabolites as an adaptive response to stress circumstances. The present study investigated the effect of different abiotic factor conditions (drought, moderate heat, severe heat, salinity, and UV-B radiation) on the essential oil (EO) yield, composition (volatile profile),...


Monitoring changes in the volatile profile of ecuadorian cocoa during different...

Erazo Solorzano, Cyntia Yadira; Tuárez García, Diego Armando; Edison Zambrano, Carlos; Moreno-Rojas, José Manuel; Rodríguez Solana, Raquel

The present work was conducted to evaluate the volatile profile of Ecuadorian Forastero, CCN-51, ETT103 and LR14 cocoa beans during traditional fermentation in laurel wood boxes followed by a sun-drying process. Fifty-six volatiles were identified with HS-SPME-GC-MS. Aldehydes, alcohols and ketones were the compounds that mainly characterized the fresh cocoa. The main compounds formed during the anaerobic ferme...


Impact of the pre-drying process on the volatile profile of on-farm processed E...

Moreno-Rojas, José Manuel; Yadira Erazo Solorzano, Cyntia; Tuárez García, Diego Armando; Pereira-Caro, Gema; Ordóñez Díaz, José Luis

Aiming to improve the quality of cocoa, preconditioning of cocoa after harvesting and before fermentation has become an on-farm processing step of great interest in recent times. The present work aimed to evaluate the influence of a pre-drying process on the volatile composition of Ecuadorian bulk (Forastero and CCN-51) and fine-flavour (ETT103 and LR14) cocoa at the end of primary processing. A total of 63 vol...


Effect of drying technique on the volatile content of ecuadorian bulk and fine-...

Erazo Solorzano, Cyntia Yadira; Disca, Vincenzo; Muñoz-Redondo, José Manuel; Tuárez García, Diego Armando; Sánchez-Parra, Mónica

Cocoa (Theobroma cacao L.) is one of the most widely consumed edible seeds in the world affected by on-farm processing. This study investigated the effect of different drying techniques, namely oven drying (OD), sun drying (SD), and a modification of sun drying using black plastic sheeting (SBPD), on the volatile profile of fine-flavor and bulk cocoa varieties analyzed by HS-SPME-GC-MS. A total of sixty-four vo...


Response of thymus lotocephalus in vitro cultures to drought stress and role of...

Mansinhos, Inês; Gonçalves, Sandra; Rodríguez-Solana, Raquel; Duarte, Hugo; Ordóñez-Díaz, José Luis; Moreno-Rojas, José Manuel; Romano, Anabela

The impact of drought stress induced by polyethylene glycol (PEG) on morphological, physiological, (bio)chemical, and biological characteristics of Thymus lotocephalus López and Morales shoot cultures have been investigated, as well as the potential of iron oxide nanoparticles, salicylic acid, and methyl jasmonate (MeJA) as alleviating drought stress agents. Results showed that PEG caused oxidative stress in a ...


Potential of bioactive compounds derived from underutilized fruit-bearing plant...

Kaur, Inderjeet; Sharma, Arun Dev; Samtiya, Mrinal; Pereira-Caro, Gema; Rodríguez Solana, Raquel; Dhewa, Tejpal; Moreno-Rojas, José Manuel

In nature, varieties of underutilized fruit-bearing plants are available and have remained underexploited for various reasons. However, the different parts of the plant, mainly the fruit, contributed considerably towards the sustainability of food as abundant sources of imperative phytochemical compounds and possess the potential for revenue generation and the conservation of ecological stability. Ethnobotanica...


Impact of temperature on Phenolic and Osmolyte contents in In Vitro cultures an...

Mansinhos, Inês; Gonçalves, Sandra; Rodríguez Solana, Raquel; Ordóñez-Díaz, José Luis; Moreno-Rojas, José Manuel; Romano, Anabela

In this study, in vitro cultures and micropropagated plants of two Mediterranean aromatic plants, Lavandula viridis L’Hér and Thymus lotocephalus López and Morales, were exposed to different temperatures (15, 20, 25, and 30 ◦C). The effect of temperature on the levels of hydrogen peroxide (H2O2 ), lipid peroxidation, and osmoprotectants (proline, soluble sugars, and soluble proteins), as well as on the phenolic...


Ultrasonic-assisted extraction and natural deep eutectic solvents combination: ...

Mansinhos, Inês; Gonçalves, Sandra; Rodríguez Solana, Raquel; Ordóñez-Díaz, José Luis; Moreno-Rojas, José Manuel; Romano, A.

The present study aimed at evaluating the effectiveness of different natural deep eutectic solvents (NADES) on the extraction of phenolic compounds from Lavandula pedunculata subsp. lusitanica (Chaytor) Franco, on the antioxidant activity, and acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and tyrosinase (Tyr) inhibitory capacities. Ten different NADES were used in this research and compared with con...


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