10 documents found, page 1 of 1
Respect and sympathy in Portuguese preschoolers and middle-school children
Costa Martins, Mariana; Cardoso, Evlyne Martins; Pires, E.; Fernandes, M.; Fernandes, C.; Malti, Tina; Veríssimo, ManuelaAbstract: Recent research has revealed theorical and empirical links between respect and socioemotional outcomes such as moral emotions and prosociality, as well as negative associations with aggression. The ability to sympathize, feel concern, and be aware of other’s states has also been hypothesized to influence how children conceptualize respect and evaluate behaviors as worthy of respect. The development of...
Molecular links between whitesand ecosystems and blackwater formation in the Ri...
Simon, C.; Pimentel, T. P.; Monteiro, M. T.F.; Candido, L. A.; Gastmans, D. [UNESP]; Geilmann, H.; da Costa Oliveira, R.; Rocha, J. B.; Pires, E.Made available in DSpace on 2022-04-29T08:31:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-10-15; Tropical rivers such as the Rio Negro constitute a major portion of the global aquatic flux of dissolved organic carbon (DOC) entering the ocean, but the exact amount, source contributions and fate of terrestrial DOC remain unknown. We investigated the role of valley and upland whitesand ecosystems (WSE...
Morphological evaluation of cardoon (Cynara cardunculus L.): assessing biodiver...
Barracosa, Paulo; Oliveira, Jorge; Barros, M.; Pires, E.Selected cardoon cultivars established in the “Serra da Estrela” region were analysed to evaluate morphological characteristics related principally to the production of cardoon flowers, which are a compulsory ingredient for particular Mediterranean PDO cheese regions. The biodiversity of twelve cardoon cultivars installed in an experimental field were evaluated over three growing seasons using thirty-four morph...
Decision Support for Energy Contracts Negotiation with Game Theory and Adaptive...
Pinto, Tiago; Vale, Zita; Praça, Isabel; Pires, E.; Lopes, FernandoThis paper presents a decision support methodology for electricity market players’ bilateral contract negotiations. The proposed model is based on the application of game theory, using artificial intelligence to enhance decision support method’s adaptive features. This model is integrated in AiD-EM (Adaptive Decision Support for Electricity Markets Negotiations), a multi-agent system that provides electricity m...
Cardosins: A new and efficient plant enzymatic tool to dissociate neuronal cell...
Duarte, A. S.; Rosa, N.; Duarte, E. P.; Pires, E.; Barros, M. T.In the present work, we examined the feasibility of using cardosins, plant aspartic-proteinases from Cynara cardunculus L., to isolate cells from rat embryonic brain. Using morphological and functional assays, we compared cell cultures obtained with cardosins with those prepared with a well-established trypsin protocol. Cardosins and trypsin dissociation produced cells with similar yield, viability, and GABA re...
Partial purification and characterisation of Candida nodaensis Killer toxin
Silva, Sónia Carina; Aguiar, Cristina; Veríssimo, P.; Pires, E.; Lucas, C.Preliminary experiments showed that C. nodaensis K factor keeps its biological activity at high NaCl concentrations and is stable after incubation in a relatively broad range of temperature and pH values, whether or not in the presence of salt. The isolation of this K toxin was already achieved by a experimental protocol involving ethanol precipitation of C. nodaensis culture supernatants. Several different app...
Identification of the cell wall receptor for Candida nodaensis Killer toxin
Silva, Sónia Carina; Aguiar, Cristina; Veríssimo, P.; Pires, E.; Lucas, CândidaThe biological action of the K toxins involves a first step in the killing process, which correspond to the adsorption the toxin to the cell wall of sensitive cells. Here we describe the work performed towards the identification of the cell wall receptor for the zymocin under this study. For this purpose, the main cell wall components of the sensitive yeast Pichia guilliermondii were extracted. These cell wall ...
The killer toxin of the halotolerant yeast Candida nodaensis
Silva, Sónia Carina; Aguiar, Cristina; Veríssimo, P.; Pires, E.; Lucas, CândidaSeveral halotolerant yeast strains, displaying a broader spectrum of K activity in the presence of salt, were identified in a previous survey. The possibility that these zymocins could be more stable than the ones described so far, lead to the selection of the extremely halotolerant yeast C. nodaensis for further work. The experiments performed to characterize C. nodaensis K factor showed that, besides keeping ...
Partial purification and characterization of Candida nodaensis killer toxin
Silva, Sónia Carina; Aguiar, Cristina; Veríssimo, P.; Pires, E.; Lucas, CândidaPreliminary experiments performed to characterize C. nodaensis K factor showed that, besides keeping its biological activity at high NaCl concentrations, is stable after incubation in a relatively broad range of temperature and pH values, whether or not in the presence of salt. In order to achieve the isolation, purification and further molecular and biochemical characterization of C. nodaensis K toxin, a exper...
The killer toxin of the halotolerant yeast Candida nodaensis
Aguiar, Cristina; Silva, Sónia Carina; Veríssimo, P.; Pires, E.; Lucas, CândidaIn a previous survey, the halotolerant yeast Candida nodaensis was identified as one of the strongest killer strains, displaying an enhancement of killer action in the presence of salt (maximum activity detected at 2M NaCl). Results of nucleic acid extractions and curing experiments suggested that this zymocin might be encoded by a nuclear gene. The great majority of killer toxins are considered labile proteins...