6 documents found, page 1 of 1
Structure-stability-function mechanistic links in the anti-measles virus action...
Figueira, Tiago Nascimento; Mendonça, Diogo A.; Gaspar, Diana; Melo, Manuel N.; Moscona, Anne; Porotto, Matteo; Castanho, Miguel A. R. B.Measles remains one of the leading causes of child mortality worldwide and is re-emerging in some countries due to poor vaccine coverage, concomitant with importation of measles virus (MV) from endemic areas. The lack of specific chemotherapy contributes to negative outcomes, especially in infants or immunodeficient individuals. Fusion inhibitor peptides derived from the MV Fusion protein C-terminal Heptad Repe...
A three-dimensional model of human lung development and disease from pluripoten...
Chen, Ya-Wen; Huang, Sarah Xuelian; de Carvalho, Ana Luisa Rodrigues Toste; Ho, Siu-Hong; Islam, Mohammad Naimul; Volpi, Stefano; Notarangelo, Luigi D.Recapitulation of lung development from human pluripotent stem cells (hPSCs) in three dimensions (3D) would allow deeper insight into human development, as well as the development of innovative strategies for disease modelling, drug discovery and regenerative medicine. We report here the generation from hPSCs of lung bud organoids (LBOs) that contain mesoderm and pulmonary endoderm and develop into branching ai...
Quantitative analysis of molecular partition towards lipid membranes using surf...
Figueira, Tiago N.; Freire, João M.; Santos, Catarina Cunha; Heras, Montserrat; Gonçalves, João; Moscona, Anne; Porotto, Matteo; Veiga, Ana SaloméUnderstanding the interplay between molecules and lipid membranes is fundamental when studying cellular and biotechnological phenomena. Partition between aqueous media and lipid membranes is key to the mechanism of action of many biomolecules and drugs. Quantifying membrane partition, through adequate and robust parameters, is thus essential. Surface Plasmon Resonance (SPR) is a powerful technique for studying ...
Quantitative analysis of molecular partition towards lipid membranes using surf...
Figueira, Tiago N.; Freire, João M.; Santos, Catarina Cunha; Heras, Montserrat; Gonçalves, João; Moscona, Anne; Porotto, Matteo; Veiga, Ana SaloméUnderstanding the interplay between molecules and lipid membranes is fundamental when studying cellular and biotechnological phenomena. Partition between aqueous media and lipid membranes is key to the mechanism of action of many biomolecules and drugs. Quantifying membrane partition, through adequate and robust parameters, is thus essential. Surface Plasmon Resonance (SPR) is a powerful technique for studying ...
Broad spectrum antiviral activity for paramyxoviruses is modulated by biophysic...
Mathieu, Cyrille; Augusto, Marcelo T.; Niewiesk, Stefan; Horvat, Branka; Palermo, Laura M.; Sanna, Giuseppina; Madeddu, Silvia; Huey, DevraHuman paramyxoviruses include global causes of lower respiratory disease like the parainfluenza viruses, as well as agents of lethal encephalitis like Nipah virus. Infection is initiated by viral glycoprotein-mediated fusion between viral and host cell membranes. Paramyxovirus viral fusion proteins (F) insert into the target cell membrane, and form a transient intermediate that pulls the viral and cell membrane...
Antiviral lipopeptide-cell membrane Interaction Is Influenced by PEG linker length
Augusto, Marcelo T.; Hollmann, Axel; Porotto, Matteo; Moscona, Anne; Santos, Nuno C.A set of lipopeptides was recently reported for their broad-spectrum antiviral activity against viruses belonging to the Paramyxoviridae family, including human parainfluenza virus type 3 and Nipah virus. Among them, the peptide with a 24-unit PEG linker connecting it to a cholesterol moiety (VG-PEG24-Chol) was found to be the best membrane fusion inhibitory peptide. Here, we evaluated the interaction of the sa...