42 documents found, page 1 of 5
Electroenzymatic CO2 Reduction Using Formate Dehydrogenase
Paul, Navendu; Moura, Isabel; Maia, Luísa B.; Cordas, Cristina M.; Moura, Jose J.G.Funding Information: This work was supported by the PTDC/BTA-BTA/0935/2020 project and by the Associate Laboratory for Green Chemistry-LAQV (UID/50006/2023), which are financed by national funds from Fundação para a Ciência e a Tecnologia, MCTES (FCT/MCTES). Publisher Copyright: © 2025 The Author(s). ChemElectroChem published by Wiley-VCH GmbH.; The electrocatalytic reduction of carbon dioxide (CO2) to formate ...
Magnetic interactions between metal sites in complex enzymes
Maiti, Biplab K.; Moura, Isabel; Moura, José J.G.Open access funding provided by FCT|FCCN (b-on). Funding from DST\u2012SERB, India for the CRG grant (CRG/2022/005673). Publisher Copyright: © The Author(s) 2025.; Magnetic interactions between iron–sulfur (Fe/S) clusters and transition metal centers such as nickel, molybdenum, and copper play a central role in the function of key metalloenzymes. These interactions, which arise from electronic coupling, spin ex...
Nitrate–Nitrite Interplay in the Nitrogen Biocycle
Maiti, Biplab K.; Moura, Isabel; Moura, José J.G.This research was funded by DST-SERB for the CRG grant (file no CRG/2022/005673) Copyright: © 2025 by the authors. Licensee MDPI, Basel, Switzerland.; The nitrogen cycle (N-cycle) is a cornerstone of global biogeochemistry, regulating nitrogen availability and affecting atmospheric chemistry, agricultural productivity, and ecological balance. Central to this cycle is the reversible interplay between nitrate (NO...
Revisiting the metal sites of nitrous oxide reductase in a low-dose structure f...
Pomowski, Anja; Dell’Acqua, Simone; Wüst, Anja; Pauleta, Sofia R.; Moura, Isabel; Einsle, OliverFunding Information: This work was supported by the Deutsche Forschungsgemeinschaft (RTG 1976, Project No. 235777276, and PP 1927, Project No. 311061829 to O.E.) and the European Research Council (Grant No. 310656 to O.E.). The authors thank Lin Zhang for the helpful discussions. Funding Information: Open Access funding enabled and organized by Projekt DEAL. This work was funded by European Molecular Biology Or...
Effect of pH on the thermostability and redox properties of cytochrome c552 fro...
Mordido, Vitor H.; Carepo, Marta S. P.; Cordas, Cristina M.; Paul, Navendu; Simon, Jörg; Moura, Isabel; Pauleta, Sofia R.Acknowledgments The authors acknowledge the Biolab for the acquisition of CD and DSC data, and Hugo Santos and the BIOSCOPE group for determining the molecular mass by LC-ESI-MS of the isolated Ws cytochrome; Cytochrome c552 from Wolinella succinogenes is one of the few examples of a low reduction potential class I c-type cytochrome with a mixture of high/low spin state populations observed in its visible spect...
Selenium
Maia, Luísa B.; Maiti, Biplab K.; Moura, Isabel; Moura, José J. G.FCT/MCTES is also acknowledged for the CEEC-Individual Program Contract (LBM). This work was also funded by DST–SERB for the CRG grant (file no CRG/2022/005673) and the Cluster University of Jammu for providing infrastructure facilities. Publisher Copyright: © 2023 by the authors.; Living organisms use selenium mainly in the form of selenocysteine in the active site of oxidoreductases. Here, selenium’s unique c...
Incorporation of a molybdenum atom in a Rubredoxin-type Centre of a de novo-des...
Bragança, Pedro M. S.; Carepo, Marta S. P.; Pauleta, Sofia R.; Pinter, Tyler; Elia, Maddalena; Cordas, Cristina M.; Moura, Isabel; Pecoraro, VicentPB would thank the PTNMRPhD (PD/00065/2013). VLP thanks the NIH for support (GM141086).; The rational design and functionalization of small, simple, and stable peptides scaffolds is an attractive avenue to mimic catalytic metal-centres of complex proteins, relevant for the design of metalloenzymes with environmental, biotechnological and health impacts. The de novo designed α3DIV-L21C framework has a rubredoxin...
Carbon Dioxide Utilisation -The Formate Route
Maia, Luisa; Moura, Isabel; Moura, José J. G.UIDB/50006/2020 CEEC-Individual 2017 Program Contract.; The relentless rise of atmospheric CO2 is causing large and unpredictable impacts on the Earth climate, due to the CO2 significant greenhouse effect, besides being responsible for the ocean acidification, with consequent huge impacts in our daily lives and in all forms of life. To stop spiral of destruction, we must actively reduce the CO2 emissions and de...
The effect of pH on Marinobacter hydrocarbonoclasticus denitrification pathway ...
Carreira, Cíntia; Nunes, Rute F.; Mestre, Olga; Moura, Isabel; Pauleta, Sofia R.PTDC/BBB-BQB/0129/2014 (IM). This work was supported by the Applied Molecular Biosciences Unit-UCIBIO, and Associate Laboratory for Green Chemistry-LAQV, which is financed by national funds from FCT (UIDB/04378/2020 and UIDB/50006/2020, respectively).; Abstract: Increasing atmospheric concentration of N2O has been a concern, as it is a potent greenhouse gas and promotes ozone layer destruction. In the N-cycle, ...
The Tetranuclear Copper-Sulfide Center of Nitrous Oxide Reductase
Pauleta, Sofia R.; Carepo, Marta S. P.; Moura, IsabelNitrous oxide reductase catalyzes the reduction of nitrous oxide (N2O) to dinitrogen (N2) and water at a catalytic tetranuclear copper sulfide center, named CuZ, overcoming the high activation energy of this reaction. In this center each Cu atom is coordinated by two imidazole rings of histidine side-chains, with the exception of one named CuIV. This enzyme has been isolated with CuZ in two forms CuZ(4Cu1S) and...