5 documents found, page 1 of 1
Single-cell profiling of MC1R-inhibited melanocytes
Berns, HM; Watkins-Chow, DE; Lu, S; Louphrasitthiphol, P; Zhang, T; Brown, KM; Moura-Alves, P; Goding, CR; Pavan, WJThe human red hair color (RHC) trait is caused by increased pheomelanin (red-yellow) and reduced eumelanin (black-brown) pigment in skin and hair due to diminished melanocortin 1 receptor (MC1R) function. In addition, individuals harboring the RHC trait are predisposed to melanoma development. While MC1R variants have been established as causative of RHC and are a well-defined risk factor for melanoma, it remai...
Development of FluoAHRL: A Novel Synthetic Fluorescent Compound That Activates ...
Jonić, N; Koprivica, I; Chatzigiannis, CM; Tsiailanis, AD; Kyrkou, SG; Tzakos, EP; Pavić, A; Dimitrijević, M; Jovanović, A; Jovanović, MB; Marinho, SAryl Hydrocarbon Receptor (AHR) ligands, upon binding, induce distinct gene expression profiles orchestrated by the AHR, leading to a spectrum of pro- or anti-inflammatory effects. In this study, we designed, synthesized and evaluated three indole-containing potential AHR ligands (FluoAHRL: AGT-4, AGT-5 and AGT-6). All synthesized compounds were shown to emit fluorescence in the near-infrared. Their AHR agonist...
Targeting the aryl hydrocarbon receptor by gut phenolic metabolites: A strategy...
Pinto, CJG; Ávila-Gálvez, MÁ; Lian, Y; Moura-Alves, P; Santos, CNThe Aryl Hydrocarbon Receptor (AHR) is a ligand-dependent transcription factor able to control complex transcriptional processes in several cell types, which has been correlated with various diseases, including inflammatory bowel diseases (IBD). Numerous studies have described different compounds as ligands of this receptor, like xenobiotics, natural compounds, and several host-derived metabolites. Dietary (pol...
Detection of Neutralizing Antibodies in COVID-19 Patients from Steve Biko Acade...
Kgatle, M; Chalwe, JM; der Westhuizen, D; Xu, ST; Damane, BP; Mathebela, P; Ueckermann, V; Mayaphi, S; Rodrigues, HG; Moura-Alves, P; Ndlovu, HA correlation between neutralization activity after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccination and protection against coronavirus disease 2019 (COVID-19) has been demonstrated by several studies. Here, we detect SARS-CoV-2 neutralizing antibody (NAB) production in COVID-19 patients from the Steve Biko Academic Hospital complex (SBAH), South Africa (SA). Samples from COVID-19 patien...
The Highly Potent AhR Agonist Picoberin Modulates Hh-Dependent Osteoblast Diffe...
Flegel, J; Shaaban, S; Jia, ZJ; Schulte, B; Lian, Y; Krzyzanowski, A; Metz, M; Schneidewind, T; Wesseler, F; Flegel, A; Reich, A; Brause, A; Xue, GIdentification and analysis of small molecule bioactivity in target-agnostic cellular assays and monitoring changes in phenotype followed by identification of the biological target are a powerful approach for the identification of novel bioactive chemical matter in particular when the monitored phenotype is disease-related and physiologically relevant. Profiling methods that enable the unbiased analysis of comp...