3 documents found, page 1 of 1
Anti-Candida targets and cytotoxicity of casuarinin isolated from Plinia caulif...
Souza-Moreira, Tatiana M. [UNESP]; Severi, Juliana A. [UNESP]; Lee, Keunsook; Preechasuth, Kanya; Santos, Emerson [UNESP]; Gow, Neil A.R.Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:29:53Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:32:42Z : No. of bitstreams: 1 2-s2.0-84880795871.pdf: 335742 bytes, checksum: 0852500dbd9c83f1aaf453c928ae8f99 (MD5); Made available in DSpace on 2014-05-27T11:29:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-07-01; In addition to the bio-guided inves...
Determination of chitin content in fungal cell wall: an alternative flow cytome...
Costa-de-Oliveira, Sofia; Silva, Ana P.; Miranda, Isabel M.; Salvador, Alexandre; Azevedo, Maria M.; Munro, Carol A.; Rodrigues, Acácio G.The conventional methods used to evaluate chitin content in fungi, such as biochemical assessment of glucosamine release after acid hydrolysis or epifluorescence microscopy, are low throughput, laborious, time-consuming, and cannot evaluate a large number of cells. We developed a flow cytometric assay, efficient, and fast, based on Calcofluor White staining to measure chitin content in yeast cells. A staining i...
Evolution of pathogenicity and sexual reproduction in eight Candida genomes
Butler, Geraldine; Rasmussen, Matthew D.; Lin, Michael F.; Santos, Manuel A. S.; Sakthikumar, Sharadha; Munro, Carol A.; Rheinbay, EstherCandida species are the most common cause of opportunistic fungal infection worldwide. Here we report the genome sequences of six Candida species and compare these and related pathogens and non-pathogens. There are significant expansions of cell wall, secreted and transporter gene families in pathogenic species, suggesting adaptations associated with virulence. Large genomic tracts are homozygous in three diplo...