8 documents found, page 1 of 1
One-Pot Microfluidics to Engineer Chitosan Nanoparticles Conjugated with Antimi...
Fonseca, D R; Alves, PM; Neto, E; Custodio, B; Guimaraes, S; Moura, D; Annis, F; Martins, M; Gomes, A; Teixeira, C; Gomes, P; Pereira, RF; Freitas, PSurface bioconjugation of antimicrobial peptides (AMP) onto nanoparticles (AMP-NP) is a complex, multistep, and time-consuming task. Herein, a microfluidic system for the one-pot production of AMP-NP was developed. Norbornene-modified chitosan was used for NP production (NorChit-NP), and thiolated-AMP was grafted on their surface via thiol-norbornene "photoclick" chemistry over exposure of two parallel UV LEDs....
Dhvar5-chitosan nanogels and their potential to improve antibiotics activity
Costa, B; Alves, PM; Fonseca, DR; Campos, F; Monteiro, AC; Shahrour, H; Gomes, A; Costa, F; Gomes, P; Martínez-de-Tejada, G; Monteiro, C; Martins, MCLInfection is one of the main causes of orthopedic implants failure, with antibiotic-resistant bacteria playing a crucial role in this outcome. In this work, antimicrobial nanogels were developed to be applied in situ as implant coating to prevent orthopedic-device-related infections. To that regard, a broad-spectrum antimicrobial peptide, Dhvar5, was grafted onto chitosan via thiol-norbornene "photoclick" chemi...
Norbornene-chitosan nanoparticles with and without a conjugated VEGF-peptide an...
Alves, PM; Fonseca, DR; Bidarra, SJ; Gomes, A; Gomes, P; Barrias, CC; Martins, MCLImpaired angiogenesis in skin chronic wounds prolongs inflammation and compromises wound healing. Several strategies have been attempted to improve vascularization, such as application of growth factors, like the vascular endothelial growth factor (VEGF). However, VEGF is expensive, has a short half-life in vivo and has been associated to tumorigenesis when used in high concentrations. QK peptide is a shorter (...
Influence of Immobilization Strategies on the Antibacterial Properties of Antim...
Barbosa, M; Alves, PM; Costa, F; Monteiro, C; Parreira, P; Teixeira, C; Gomes, P; Martins, MCLIt is key to fight bacterial adhesion to prevent biofilm establishment on biomaterials. Surface immobilization of antimicrobial peptides (AMP) is a promising strategy to avoid bacterial colonization. This work aimed to investigate whether the direct surface immobilization of Dhvar5, an AMP with head-to-tail amphipathicity, would improve the antimicrobial activity of chitosan ultrathin coatings. The peptide was ...
Thiol-Norbornene Photoclick Chemistry for Grafting Antimicrobial Peptides onto ...
Alves, PM; Pereira, RF; Costa, B; Tassi, N; Teixeira, C; Leiro, V; Monteiro, C; Gomes, P; Costa, F; Martins, MCLCovalent conjugation of antimicrobial peptides (AMP) is considered an effective approach to overcome their potential degradation in vivo. As the high cost of AMP-based therapies is one of the major hurdles towards their clinical application, it is urgently needed to explore high yield reactions. Herein, the highly efficient thiol-norbornene photoclick chemistry (TNPC) was explored for AMP grafting onto chitosan...
Norbornene-chitosan spray-dried microspheres for peptide conjugation using thio...
Alves, PM; Fonseca, DR; Pereira, RF; Estevinho, BN; Teixeira, C; Gome, P; Martins, MCLThe action of bioactive peptides, such as antimicrobial peptides (AMP), in the human body is often compromised by limited residence time and stability in the target site. Bioconjugation of peptides to biomaterial surfaces is one of the strategies that may overcome these limitations. Herein, norbornene-chitosan (NorChit) microspheres were engineered to react with thiolated peptides by thiolene “photoclick” chemi...
Prognostic Value of Isolated Elevated Troponin I Levels in Patients without Acu...
Domingues, C; Ferreira, MJ; Ferreira, JM; Marinho, AV; Alves, PM; Ferreira, C; Fonseca, I; Gonçalves, LBackground: Although non-ischemic troponin elevation is frequently seen in patients admitted to the emergency department (ED), consensus regarding its management is lacking. Objectives: This study aimed to characterize patients admitted to the ED with non-ischemic troponin elevation and to identify potential mortality predictors in this population. Methods: This retrospective observational study included ED pat...
Smart biomaterial-based systems for intrinsic stimuli-responsive chronic wound ...
Alves, PM; Barrias, CC; Gomes, P; Martins, MCLSkin chronic wounds are associated with a state of persistent inflammation and often with infection, originating a specific microenvironment characterized by increased temperature, alkaline pH, elevated enzymatic activity, and high levels of reactive oxygen species (ROS). These alterations can be explored as intrinsic triggers in the design of stimuli-responsive biomaterials for the release of bioactive molecul...