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An antibody-based survey of Toxoplasma gondii and Neospora caninum infection in...

Aires Pereira, M; Nóbrega, C; Mateus, TL; Almeida, D; Oliveira, A; Coelho, C; Cruz, R; Oliveira, PA; Faustino-Rocha, AI; Pires, MJ; Mesquita, JR

Toxoplasma gondii and Neospora caninum are intracellular parasites with a great impact on human and animal health, respectively. This work aims to investigate the presence of antibodies against T. gondii and N. caninum in client-owned cats from Portugal and to identify risk factors. A total of 183 domestic cats were sampled and their owners answered an online questionnaire designed to obtain background informat...


Pulmonary and Gastrointestinal Parasitic Infections in Small Ruminant Autochtho...

Pereira, MA; Vila-Viçosa, MJ; Coelho, C; Santos, C; Esteves, F; Cruz, R; Gomes, L; Henriques, D; Vala, H; Nóbrega, C; Mega, AC; Melo, C; Malva, M

The production of small ruminant autochthonous breeds in the Centre region of Portugal is practiced in a semi-extensive husbandry system, exposing animals to parasitic infections. The main objective of this study was to estimate the prevalence of lungworm infection and identify risk factors. Fecal samples of 203 goats and 208 sheep from 30 herds were collected per rectum and subjected to the modified Baermann t...


Molecular evidence of sporadic Coxiella burnetii excretion in sheep milk, centr...

Pires, H; Santos-Silva, S; Cruz, AVS; Cardoso, L; Lopes, AP; Pereira, MA; Nóbrega, C; Mega, AC; Santos, C; Cruz, R; Esteves, F; Vala, H; Matos, AC

Coxiella burnetii is the etiologic agent of Q fever, a worldwide zoonosis. Cattle, sheep and goats are considered the main reservoirs of the disease. Transmission to humans occurs mainly through the inhalation of infectious aerosols from milk, faeces, urine, and birth products from infected ruminants. In this study, a 2-year longitudinal approach was performed to ascertain the excretion of C. burnetii in bulk t...


An Antibody-Based Survey of Toxoplasma gondii and Neospora caninum Infection in...

Pereira, MA; Nóbrega, C; Mateus, TL; Almeida, D; Oliveira, A; Coelho, C; Cruz, R; Oliveira, P; Faustino-Rocha, A; Pires, MJ; Mesquita, JR; Vala, H

Simple Summary Toxoplasma gondii and Neospora caninum are intracellular parasites with a great impact on human and animal health, respectively. This work aims to investigate the presence of antibodies against T. gondii and N. caninum in client-owned cats from Portugal and to identify risk factors. A total of 183 domestic cats were sampled and their owners answered an online questionnaire designed to obtain back...


Virtual physiology: a tool for the 21st Century

Nóbrega, C; Pereira, MA; Coelho C; Brás, C; Mega, AC; Santos, C; Esteves, F; Cruz, R; Faustino-Rocha, AI; Oliveira, PA; Mesquita, J; Vala, H

Veterinary physiology is a basic curricular unit for every course within the veterinary field. It is mandatory to understand how the animal body works, and what to expect of a healthy body, in order to recognize any misfunction, and to be able to treat it. Classic physiology teaching involves wet labs, much equipment, many reagents, some animals, and a lot of time. But times are changing. In the 21st century, i...


A Questionnaire-Based Survey on the Long-Term Management of Canine Leishmaniosi...

Pereira, MA; Santos, R; Nóbrega, C; Mega, C; Cruz, R; Esteves, F; Santos, C; Coelho, C; Mesquita, JR; Vala, H; Santos-Gomes, G

Canine Leishmaniosis (CanL) is a chronic and potentially fatal disease. In economically disadvantaged regions, costs associated with long-term patient monitoring may determine that some owners decline veterinary follow-up of their dogs. This online, questionnaire-based survey aimed to assess how Portuguese veterinary practitioners perform long-term patient monitoring and recognize relapses. More than 50% of res...


Crimean-Congo hemorrhagic fever virus circulating among sheep of Portugal: a na...

Mesquita, JR; Cruz, R; Esteves, F; Santos, C; Pousa, H; Coelho, C; Mega, AC; Nóbrega, C; Vala, H; Peyrefitte, CN; Nascimento, MSJ; Barradas, PF

Crimean-Congo hemorrhagic fever virus (CCHFV) is a widespread zoonotic pathogen that can cause mild to severe hemorrhagic disease in humans. CCHFV may be transmitted through direct contact with tissue or blood of viremic animals; however, the primary transmission route is through infected tick bites. CCHFV RNA has been detected in ticks feeding on domestic and wild animals in western Spain, suggesting an establ...


A 2-Year Longitudinal Seroepidemiological Evaluation of Toxoplasma gondii Antib...

Almeida, D; Quirino, J; Barradas, PF; da Silva, PG; Pereira, M; Cruz, R; Santos, C; Mega, AC; Esteves, F; Nóbrega, C; Vala, H; Gärtner, F; Amorim, I

(1) Background: Toxoplasma gondii is an important zoonosis and one of the major causes of abortion in sheep worldwide. (2) Methods: We performed a 2-year longitudinal serological anti-T. gondii IgG screening on a cohort of a spatially confined population of a Portuguese autochthonous sheep breed in central Portugal. (3) Results: From the screening of the 2015 and 2016 sera, an increase of seroprevalence was obs...


Occurrence and Distribution of Fasciolosis in a Cohort of Ovine Livestock Restr...

Coelho, C; Cruz, R; Esteves, F; Vala, H; Pereira, MA; Amorim, I; Nóbrega, C; Mesquita, JR

Fasciola hepatica is a parasite that is widespread in Europe, having been reported in ruminants of several European countries and causing an important economic impact. This study ascertained the occurrence and distribution of fasciolosis in Portuguese ovine livestock by assessing F. hepatica IgG antibodies in a cohort of confined sheep from a high-altitude region of central Portugal in a 2-year period. Positive...


Catatonia in Anti-N-Methyl-D-Aspartate (NMDA) Receptor Encephalitis Misdiagnose...

Ponte, A; Brito, A; Nóbrega, C; Pinheiro, S; Gama Marques, J

Anti-N-Mmethyl-D-aspartate receptor encephalitis is an autoimmune disease of the central nervous system with prominent neurologic and psychiatric features. Symptoms appear progressively and sometimes with an exclusively psychiatric initial presentation. The patient's evaluation should be meticulous, and we should use all the diagnostic tests required for the exclusion of entities that can mimic this disease. We...


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