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Surtos de mpox em Portugal: resposta laboratorial e desafios no controlo da epi...

Cordeiro, Rita; Francisco, Rafaela; Pelerito, Ana; Carvalho, Isabel Lopes de; Núncio, Maria Sofia

A infeção mpox, causada pelo vírus monkeypox (MPXV), tornou-se uma emergência global de saúde pública em 2022. Portugal foi um dos primeiros países a confirmar casos de infeção, o que levou à implementação de um sistema de vigilância laboratorial a nível nacional. Este estudo descreve a evolução epidemiológica de mpox em Portugal entre 2022 e 2025 e avalia o desempenho de diferentes tipos de amostras biológicas...


Viral genetics and transmission dynamics in the second wave of mpox outbreak in...

Cordeiro, Rita; Caetano, Constantino P.; Sobral, Daniel; Ferreira, Rita; Coelho, Luís; Pelerito, Ana; de Carvalho, Isabel Lopes; Namorado, Sónia


An Overview of Monkeypox Virus Detection in Different Clinical Samples and Anal...

Cordeiro, Rita; Pelerito, Ana; de Carvalho, Isabel Lopes; Lopo, Sílvia; Neves, Raquel; Rocha, Raquel; Palminha, Paula; Verdasca, Nuno

Mpox is a zoonotic disease caused by the Monkeypox virus (MPXV), and since May 2022, tens of thousands of cases have been reported in non-endemic countries. We aimed to evaluate the suitability of different sample types for mpox diagnostic and assess the temporal dynamics of viral load. We evaluated 1914 samples from 953 laboratory-confirmed cases. The positivity rate was higher for lesion (91.3%) and rectal sw...


Natural selection and recombination at host-interacting lipoprotein loci drive ...

Akther, Saymon; Mongodin, Emmanuel F.; Morgan, Richard D.; Di, Lia; Yang, Xiaohua; Golovchenko, Maryna; Rudenko, Natalie; Margos, Gabriele

Lyme disease, caused by spirochetes in the Borrelia burgdorferi sensu lato clade within the Borrelia genus, is transmitted by Ixodes ticks and is currently the most prevalent and rapidly expanding tick-borne disease in Europe and North America. We report complete genome sequences of 47 isolates that encompass all established species in this clade while highlighting the diversity of the widespread human pathogen...


REVIVE 2023 e riscos para a saúde pública em Portugal: vigilância de carraças e...

Carvalho, Isabel Lopes de; Sousa, Rita de; Luz, Teresa; Parreira, Paulo; Gomes, Maria Salomé; Soares, Patrícia; Zé-Zé, Líbia; Osório, Hugo Costa

As carraças são um dos vetores mais importantes de agentes patogénicos para o Homem, com impacto crescente em saúde pública em todo o mundo. Os agentes infeciosos transmitidos por carraças incluem vírus, como o vírus da encefalite transmitida por carraças e da febre hemorrágica Crimeia-Congo, bactérias, tais como os agentes etiológicos da borreliose de Lyme, febre escaro nodular, outras rickettsioses e anaplasm...


Viral genetic clustering and transmission dynamics of the 2022 mpox outbreak in...

Borges, Vítor; Duque, Mariana Perez; Martins, João Vieira; Vasconcelos, Paula; Ferreira, Rita; Sobral, Daniel; Pelerito, Ana; de Carvalho, Isabel Lopes

Pathogen genome sequencing during epidemics enhances our ability to identify and understand suspected clusters and investigate their relationships. Here, we combine genomic and epidemiological data of the 2022 mpox outbreak to better understand early viral spread, diversification and transmission dynamics. By sequencing 52% of the confirmed cases in Portugal, we identified the mpox virus sublineages with the hi...


Viral genetic clustering and transmission dynamics of the 2022 mpox outbreak in...

Borges, Vítor; Duque, Mariana Perez; Martins, João Vieira; Vasconcelos, Paula; Ferreira, Rita; Sobral, Daniel; Pelerito, Ana; de Carvalho, Isabel Lopes

Pathogen genome sequencing during epidemics enhances our ability to identify and understand suspected clusters and investigate their relationships. Here, we combine genomic and epidemiological data of the 2022 mpox outbreak to better understand early viral spread, diversification and transmission dynamics. By sequencing 52% of the confirmed cases in Portugal, we identified the mpox virus sublineages with the hi...


Human immunodeficiency virus infection may be a contributing factor to monkeypo...

Alpalhão, Miguel; Sousa, Diogo; Frade, Joana Vieitez; Patrocínio, João; Garrido, Pedro Miguel; Correia, Catarina; Brazão, Cláudia; Mancha, Dora

To the Editor: An outbreak of monkeypox has emerged, and more than 13,000 cases have already been confirmed worldwide. In our department, we have 42 confirmed cases so far. All of them are cisgender males presenting with lesions in the genital, perianal, or perioral areas (Fig 1). Interestingly, we observe a disproportionate number of individuals living with the human immunodeficiency virus (HIV). Below, we pre...


Diversity of microorganisms in Hyalomma aegyptium collected from spur-thighed t...

Norte, Ana Cláudia; Harris, David James; Silveira, Diogo; Nunes, Carolina Saramago; Núncio, Maria Sofia; Martínez, Eva Graciá; Giménez, Andrés

Ticks carry a diverse community of microorganisms including non-pathogenic symbionts, commensals, and pathogens, such as viruses, bacteria, protozoans, and fungi. The assessment of tick-borne microorganisms (TBM) in tortoises and their ticks is essential to understand their eco-epidemiology, and to map and monitor potential pathogens to humans and other animals. The aim of this study was to characterize the div...


Monkeypox virus infection in HIV1‐coinfected patients previously vaccinated aga...

Brazão, Cláudia; Garrido, Pedro; Alpalhão, Miguel; Roda, Ângela; Vieitez Frade, Joana; Ferreira, João; Pelerito, Ana; Carvalho, Isabel Lopes

Monkeypox is a zoonotic orthopoxvirus that incidentally causes disease in humans similar to smallpox, although with lower mortality. This virus is endemic to western and central Africa, with outbreaks in occidental countries related to exotic pet trade and international travel. Coincident immunity to monkeypox virus was previously achieved with vaccinia virus vaccination; however, eradicating smallpox and subse...


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