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Accelerated Development of AAV Purification Process Using a High-Throughput and...
Mendes, Sónia; Faria, Tiago Q.; Nascimento, André; Noverraz, Marc; Bollmann, Franziska; Nestola, Piergiuseppe; Roldão, António; Peixoto, CristinaFunding Information: This work was funded by the Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES, Portugal) through national funds to iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020), the Associate Laboratory LS4FUTURE (LA/P/0087/2020) and projects PTDC/EQU-EQU/0142/2020 and EXPL/EQU-EQU/1567/2021. Publisher Copyright: © 2024 by the authors.; Adeno-associate...
Model-based design and optimization of GSSR chromatography for peptide purifica...
Santos, Tiago P. D.; Fernandes, Rita P.; Ribeiro, Rui P. P. L.; Peixoto, Cristina; Mota, José P. B.PhD grant BD/06003/2020 (R.P. Fernandes). Publisher Copyright: © 2022 The Authors; Gradient with Steady State Recycle (GSSR) is a recently developed process for center-cut separation by solvent-gradient chromatography. The process comprises a multicolumn, open-loop system with cyclic steady-state operation that simulates a solvent gradient moving countercurrently with respect to the solid phase. However, the fe...
Efficient adeno-associated virus serotype 5 capture with affinity functionalize...
Neto, Salomé; Mendes, João P.; Santos, Susana B.Dos; Solbrand, Anita; Carrondo, Manuel J.T.; Peixoto, Cristina; Silva, Ricardo J.S.Funding Information: The authors acknowledge the funding of Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES, Portugal) through national funds to iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020), projects PTDC/EQU-EQU/0142/2020 and EXPL/EQU-EQU/1567/2021; SN is the recipient of an FCT fellowship from the project PTDC/EQU-EQU/0142/2020. Publisher Copyright: Co...
Implementation of Novel Affinity Ligand for Lentiviral Vector Purification
Moreira, Ana Sofia; Bezemer, Sandra; Faria, Tiago Q.; Detmers, Frank; Hermans, Pim; Sierkstra, Laurens; Coroadinha, Ana Sofia; Peixoto, CristinaFunding Information: The authors would like to acknowledge funding from Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES, Portugal) through national funds to iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020) and the Associate Laboratory LS4FUTURE (LA/P/0087/2020). The author Ana Sofia Moreira acknowledges FCT/MCES for the PhD fellowship PD/BD/135501/2018. Publ...
Extending AAV Packaging Cargo through Dual Co-Transduction
Ferreira, Mariana V.; Fernandes, Sofia; Almeida, Ana Isabel; Neto, Salomé; Mendes, João P.; Silva, Ricardo J.S.; Peixoto, CristinaFunding Information: Author Mariana V. Ferreira acknowledges Fundação para a Ciência e Tecnologia for PH.D. fellowship UI/BD/151256/2021 within the scope of the Ph.D. Program in Bioengineering—Cell Therapies and Regenerative Medicine. This work was funded by Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES, Portugal) through national funds to iNOVA4Health (UIDB...
Clinical-grade oncolytic adenovirus purification using polysorbate 20 as an alt...
Moleirinho, Mafalda G.; Rosa, Sara; Carrondo, Manuel J.T.; Silva, Ricardo J. S.; Hagner-McWhirter, Åsa; Ahlén, Gustaf; Lundgren, Mats; Alves, Paula M.PD/BD/114034/2015; Introduction: Oncolytic virus therapy is currently considered as a promising therapeutic approach for cancer treatment. Adenovirus is well-known and extensively characterized as an oncolytic agent. The increasing number of clinical trials using this virus generates the demand for the development of a well-established purification approach. Triton X-100 is commonly used in cell lysis buffer pr...
A detection and quantification label-free tool to speed up downstream processin...
Carvalho, Sofia B.; Moreira, Ana Sofia; Gomes, Joana; Carrondo, Manuel J.T.; Thornton, David J.; Alves, Paula M.; Costa, Julia; Peixoto, CristinaMucins are high-molecular weight glycoproteins (0.25–20 MDa) containing one or more domains that are heavily O-glycosylated. Their implications as targets for cancer treatment have increased the interest in these glycoproteins, mainly in the fields of vaccines and antibodies. However, mucins present high heterogeneity, posing challenges that affect purification processes and quality control analysis. In that se...