14 documents found, page 1 of 2
Single-cell liquid-core microcapsules for biomedical applications
Pires-Santos, Manuel; Carreira, Mariana; Morais, Bruno P.; Perfeito, Francisca G.; Oliveira, Mariana B.; Monteiro, Cátia F.; Nadine, Sara; Mano, João F.More recently, single-cell encapsulation emerged as a promising field in biomedicine due to its potential applications, in cell analysis and therapy. Traditional techniques involve embedding cells in crosslinked polymers to create continuous microgels, suitable mainly for adherent cells, or encapsulating them in droplets for only short-term analysis, due to their instability. In this study, we developed a metho...
Unveiling the potential of single‐cell encapsulation in biomedical applications...
Pires‐Santos, Manuel; Nadine, Sara; Mano, João F.The encapsulation of single cells has emerged as a promising field in recent years, owing to its potential applications in cell-based therapeutics, bioprinting, in vitro cell culture, high-throughput screening, and diagnostics. Single-cell units offer several advantages, including compatibility with standard imaging techniques, superior diffusion rates, and lower material-to-cell volume ratios. They also serve ...
Liquefied capsules containing nanogrooved microdiscs and umbilical cord-derived...
Carreira, Mariana; Pires-Santos, Manuel; Correia, Clara R.; Nadine, Sara; Mano, João F.Background: Surface topography has been shown to influence cell behavior and direct stromal cell differentiation into distinct lineages. Whereas this phenomenon has been verified in twodimensional cultures, there is an urgent need for a thorough investigation of topography’s role within a three-dimensional (3D) environment, as it better replicates the natural cellular environment. Methods: A co-culture of Whart...
Strategies to decouple cell micro-scale and macro-scale environments for design...
Ghasemzadeh-Hasankolaei, Maryam; Pinheiro, Diogo; Nadine, Sara; Mano, João F.The regulation of cellular behavior within a three-dimensional (3D) environment to execute a specific function remains a challenge in the field of tissue engineering. In native tissues, cells and matrices are arranged into 3D modular units, comprising biochemical and biophysical signals that orchestrate specific cellular activities. Modular tissue engineering aims to emulate this natural complexity through the ...
Bioengineering the human bone marrow microenvironment in liquefied compartments...
Oliveira, Cláudia S.; Nadine, Sara; Gomes, Maria C.; Correia, Clara R.; Mano, João F.Recreating the biological complexity of living bone marrow (BM) in a single in vitro strategy has faced many challenges. Most bioengineered strategies propose the co-culture of BM cellular components entrapped in different matrices limiting their migration and self-organization capacity or in open scaffolds enabling their escaping. We propose a methodology for fabricating a "human bone marrow-in-a-liquefied-cap...
Liquefied microcapsules compartmentalizing macrophages and umbilical cord-deriv...
Nadine, Sara; Fernandes, Inês; Patrício, Sónia G.; Correia, Clara R.; Mano, João F.Extraordinary capabilities underlie the potential use of immune cells, particularly macrophages, in bone tissue engineering. Indeed, the depletion of macrophages during bone repair often culminates in disease scenarios. Inspired by the native dynamics between immune and skeletal systems, this work proposes a straightforward in vitro method to bioengineer biomimetic bone niches using biological waste. For that, ...
Close-to-native bone repair via tissue-engineered endochondral ossification app...
Nadine, Sara; Fernandes, Inês J.; Correia, Clara R.; Mano, João F.In order to solve the clinical challenges related to bone grafting, several tissue engineering (TE) strategies have been proposed to repair critical-sized defects. Generally, the classical TE approaches are designed to promote bone repair via intramembranous ossification. Although promising, strategies that direct the osteogenic differentiation of mesenchymal stem/stromal cells are usually characterized by a la...
Engineering immunomodulatory hydrogels and cell-laden systems towards bone rege...
Nadine, Sara; Correia, Clara R.; Mano, João F.The well-known synergetic interplay between the skeletal and immune systems has changed the design of advanced bone tissue engineering strategies. The immune system is essential during the bone lifetime, with macrophages playing multiple roles in bone healing and biomaterial integration. If in the past, the most valuable aspect of implants was to avoid immune responses of the host, nowadays, it is well-establis...
Minimalist tissue engineering approaches using low material-based bioengineered...
Correia, Clara; Bjorge, Isabel M.; Nadine, Sara; Mano, João F.From an “over-engineering” era in which biomaterials played a central role, now it is observed to the emergence of “developmental” tissue engineering (TE) strategies which rely on an integrative cell-material perspective that paves the way for cell self-organization. The current challenge is to engineer the microenvironment without hampering the spontaneous collective arrangement ability of cells, while simulta...
An immunomodulatory miniaturized 3D screening platform using liquefied capsules
Nadine, Sara; Correia, Clara R.; Mano, João F.A critical determinant of successful clinical outcomes is the host's response to the biomaterial. Therefore, the prediction of the immunomodulatory bioperformance of biomedical devices following implantation is of utmost importance. Herein, liquefied capsules are proposed as immunomodulatory miniaturized 3D platforms for the high-content combinatorial screening of different polymers that could be used generical...