6 documents found, page 1 of 1
Secretory production in Escherichia coli of a GH46 chitosanase from Chromobacte...
Azevedo, Mayara I. G.; Oliveira, Simone T.; Silva, Christiana F. B.; Carneiro, Rômulo F.; Nagano, Celso S.; Gadelha, Ana Clarice da SilvaA chitosanase (CvCsn46) from Chromobacterium violaceum ATCC 12472 was produced in Escherichia coli, purified, and partially characterized. When subjected to denaturing polyacrylamide gel electrophoresis, the enzyme migrated as two protein bands (38 and 36 kDa apparent molecular masses), which were both identified as CvCsn46 by mass spectrometry. The enzyme hydrolyzed colloidal chitosan, with optimum catalytic a...
Structural and functional features of a class VI chitinase from cashew (Anacard...
Oliveira, Simone T.; Azevedo, Mayara I. G.; Cunha, Rodrigo M. S.; Silva, Christiana F. B.; Muniz, Celli R.; Monteiro-Júnior, José E.A partial cDNA sequence from Anacardium occidentale CCP 76 was obtained, encoding a GH19 chitinase (AoChi) belonging to class VI. AoChi exhibits distinct structural features in relation to previously characterized plant GH19 chitinases from classes I, II, IV and VII. For example, a conserved Glu residue at the catalytic center of typical GH19 chitinases, which acts as the proton donor during catalysis, is repla...
Cloning of cDNA sequences encoding cowpea (Vigna unguiculata) vicilins: Computa...
Rocha, Antônio J.; Sousa, Bruno L.; Girão, Matheus S.; Barroso-Neto, Ito L.; Júnior, José E. Monteiro; Oliveira, José Tadeu Abreu de; Nagano, Celso S.Vicilins are 7S globulins which constitute the major seed storage proteins in leguminous species. Variant vicilins showing differential binding affinities for chitin have been implicated in the resistance and susceptibility of cowpea to the bruchid Callosobruchus maculatus. These proteins are members of the cupin superfamily, which includes a wide variety of enzymes and non-catalytic seed storage proteins. The ...
Protein profile of the ovarian follicular fluid of brown brocket deer (Mazama g...
Souza, Thais T. S.; Bezerra, Maria J. B.; van Tilburg, Mauricio F.; Nagano, Celso S.; Rola, Luciana D. [UNESP]; Duarte, Jose M. B. [UNESP]Made available in DSpace on 2021-06-25T11:23:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-04-01; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); (FUNCAP); The aim of this study was to characterize the protein profile of ovarian follicular fluid (FF) of brown brocket deer (Mazama gouazoubira). Five adult females received an ovarian stimulation treatment and the FF was collected...
Modulation of the pharmacological effects of enzymatically-active PLA 2 by BTL-...
Oliveira, Simone C.B.; Fonseca, Fabiana V.; Antunes, Edson; Camargo, Enilton A.; Morganti, Rafael P.; Aparício, Ricardo; Toyama, Daniela O.Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:23:36Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:40:47Z : No. of bitstreams: 1 2-s2.0-46649109942.pdf: 446690 bytes, checksum: 87558c09dd78812dbd892f265a25a877 (MD5); Made available in DSpace on 2014-05-27T11:23:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-07-11; Background. An interaction between ...
cDNA cloning and 1.75 Å crystal structure determination of PPL2, an endochitina...
Cavada, Benildo S.; Moreno, Frederico B. B. [UNESP]; Da Rocha, Bruno A. M.; De Azevedo Jr., Walter F.; Castellón, Rolando E. R.; Goersch, Georg V.Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:21:57Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:34:48Z : No. of bitstreams: 1 2-s2.0-33747413686.pdf: 511841 bytes, checksum: 1e5bb0ef6e0943c719824a16bc330739 (MD5); Made available in DSpace on 2014-05-27T11:21:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-09-01; Parkia platycephala lectin 2 was pu...