5 documents found, page 1 of 1
Purification, biochemical characterization, and amino acid sequence of a novel ...
Carneiro, Rômulo Farias; Torres, Renato Cézar Farias; Chaves, Renata Pinheiro; Vasconcelos, Mayron Alves de; Sousa, Bruno Lopes deA new lectin from Aplysia dactylomela eggs (ADEL) was isolated by affinity chromatography on HClactivated Sepharose™ media. Hemagglutination caused by ADEL was inhibited by several galactosides, mainly galacturonic acid (Ka = 6.05 × 106 M−1 ). The primary structure of ADEL consists of 217 residues, including 11 halfcystines involved in five intrachain and one interchain disulfide bond, resulting in a molecular ...
Morphology, ultrastructure and immunocytochemistry of Hypnea cervicornis and Hy...
Miguel, Thaiz Batista Azevedo Rangel; Schmidt, Eder C.; Bouzon, Zenilda Laurita; Nascimento, Fernando E.P.; Cunha, Maura da; Fernandes, Saulo PiredaBased on their morphological and physiological features, red algae comprise a complex and variable group of multiple genera, including Hypnea. In particular, the genus Hypnea J.V. Lamouroux (Cystocloniaceae, Rhodophyta) consists of approximately 54 species, including Hypnea cervicornis and H. musciformis. Lectins were described for both species; however, the localization of these proteins is still unclear. Ther...
Purification, partial characterization and immobilization of a mannose-specific...
Pinto Júnior, Vanir Reis; Santiago, Mayara Queiroz de; Osterne, Vinícius José da Silva; Correia, Jorge Luis AlmeidaLectin from the seeds of Dioclea lasiophylla (DlyL) was purified in a single step by affinity chromatography on a Sephadex® G-50 column. DlyL strongly agglutinated rabbit erythrocytes and was inhibited by monosaccharides (D-mannose and α-methyl-Dmannoside) and glycoproteins (ovalbumin and fetuin). Similar to other Diocleinae lectins, DlyL has three chains, α, β and γ, with mass of 25,569 ± 2, 12,998 ± 1 and 12,...
Purificação e caracterização de uma hemaglutinina da alga marinha vermelha Hypn...
Nagano, Celso ShinitiO presente trabalho teve como objetivo a purificação e caracterização parcial da lectina presente na alga marinha vermelha Hypnea musciformis (Wulfen) Lamouroux, coletada nos recifes de maré na Praia do Pacheco, Município de Caucaia, Estado do Ceará. As algas coletadas foram maceradas e transformadas em um fino pó com auxilio de nitrogênio liquido e utilizadas para o preparo de um extrato protéico, seguido de p...
Purification of a PHA-like chitin-binding protein from Acacia farnesiana seeds ...
Santi-Gadelha, Tatiane; Rocha, Bruno Anderson Matias; Oliveira, Cecília Carvalho; Aragão, Karoline Sabóia; Marinho, EEmmanuel SilvaA lectin-like protein from the seeds of Acacia farnesiana was isolated from the albumin fraction, characterized, and sequenced by tandem mass spectrometry. The albumin fraction was extracted with 0.5 M NaCl, and the lectin-like protein of A. farnesiana (AFAL) was purified by ion-exchange chromatography (Mono-Q) followed by chromatofocusing. AFAL agglutinated rabbit erythrocytes and did not agglutinate human ABO...