7 documents found, page 1 of 1
Microbiome and transcriptome ontogeny with a focus on the fish immune system: i...
Najafpour, BabakAquaculture hatcheries face persistent challenges concerning poor larvae quality and high mortality rates. The early developmental stages and the transition from larvae to juveniles are critical periods characterized by heightened vulnerability to environmental stressors. Researchers have turned to next-generation sequencing techniques, which have provided invaluable insights into organism biology and symbiotic...
Core microbiome profiles and their modification by environmental, biological, a...
Najafpour, Babak; Pinto, Patricia IS; Sanz, Eric Climent; Martinez-Blanch, Juan F.; Canario, Adelino; Moutou, Katerina A.; Power, Deborah16S rRNA gene sequencing and bacteria-and genus-specific quantitative PCR was used to profile microbial communities and their associated functions in water, live feed (microalgae, Artemia, and rotifer), and European sea bass and gilthead sea bream larvae from hatcheries in Greece and Italy. The transfer to larvae of genus containing potential pathogens of fish was more likely with Artemia and rotifer than with ...
Quantifying dominant bacterial genera detected in metagenomic data from fish eg...
Najafpour, Babak; Canario, Adelino; Power, Deborah; Pinto, Patricia ISThe goal of this study was to design genus-specific primers for rapid evaluation of the most abundant bacterial genera identified using amplicon-based sequencing of the 16S rRNA gene in fish-related samples and surrounding water. Efficient genus-specific primers were designed for 11 bacterial genera including Alkalimarinus, Colwellia, Enterovibrio, Marinomonas, Massilia, Oleispira, Phaeobacter, Photobacterium, ...
Factors driving bacterial microbiota of eggs from commercial hatcheries of Euro...
Najafpour, Babak; Pinto, Patricia IS; Moutou, Katerina A.; Canario, A.V.M.; Power, Deborah MaryA comprehensive understanding of how bacterial community abundance changes in fishes during their lifecycle and the role of the microbiota on health and production is still lacking. From this perspective, the egg bacterial communities of two commercially farmed species, the European seabass (Dicentrarchus labrax) and the gilthead seabream (Sparus aurata), from different aquaculture sites were compared, and the ...
Comparative ontogeny of the digestive tract of Oncorhynchus mykiss female x Sal...
Najafpour, Babak; Dorafshan, Salar; Paykan Heyrati, Fatemeh; Canario, Adelino; Power, Deborahthe ontogeny of the gastrointestinal tract of the hybrid between female rainbow trout, RT (Oncorhynchus mykiss) and male Caspian brown trout, CBT (Salmo trutta caspius) was compared to the parental species. Larvae were collected for histology and enzymatic assays (amylase, lipase and trypsin) at 3, 6, 9, 12, 15, 18, 21, 26, 31, 35, 40 and 45 days post-hatch (dph). the development of the gastrointestinal tract (...
Specific evolution and gene family expansion of complement 3 and regulatory fac...
Najafpour, Babak; Cardoso, João CR; Canario, Adelino; Power, DeborahThe complement system comprises a large family of plasma proteins that play a central role in innate and adaptive immunity. To better understand the evolution of the complement system in vertebrates and the contribution of complement to fish immunity comprehensive in silico and expression analysis of the gene repertoire was made. Particular attention was given to C3 and the evolutionary related proteins C4 and ...
Embryonic development of the endangered Caspian brown trout, Salmo trutta caspi...
Najafpour, Babak; Dorafshan, Salar; Heyrati, Fatemeh Paykan; Power, DeborahThe Caspian brown trout, Salmo trutta caspius is an endangered species of economic value and better knowledge of its biology will contribute to conservation measures. In the present study we characterized its early development to identify critical embryonic stages, by observing embryos and larvae from a broodstock maintained in captivity incubated at 9 +/- 1 degrees C. Morphological analysis of landmark stages ...