11 documents found, page 1 of 2
Antibiotic resistance in European wastewater treatment plants mirrors the patte...
Pärnänen, Katariina M. M.; Narciso-da-Rocha, Carlos; Kneis, David; Berendonk, Thomas U.; Cacace, Damiano; Do, Thi Thuy; Elpers, ChristianIntegrated antibiotic resistance (AR) surveillance is one of the objectives of the World Health Organization global action plan on antimicrobial resistance. Urban wastewater treatment plants (UWTPs) are among the most important receptors and sources of environmental AR. On the basis of the consistent observation of an increasing north-to-south clinical AR prevalence in Europe, this study compared the influent a...
Solar treatment (H2O2, TiO2-P25 and GO-TiO2 photocatalysis, photo-Fenton) of or...
Moreira, Nuno F. F.; Narciso-da-Rocha, Carlos; Pólo-Lopez, M. Immaculada; Pastrana-Martínez, Luisa M.; Faria, Joaquim L.; Manaia, Célia M.Solar-driven advanced oxidation processes were studied in a pilot-scale photoreactor, as tertiary treatments of effluents from an urban wastewater treatment plant. Solar-H2O2, heterogeneous photocatalysis (with and/or without the addition of H2O2 and employing three different photocatalysts) and the photo-Fenton process were investigated. Chemical sulfamethoxazole, carbamazepine, and diclofenac) and biological ...
Bacterial lineages putatively associated with the dissemination of antibiotic r...
Narciso-da-Rocha, Carlos; Rocha, Jaqueline; Vaz-Moreira, Ivone; Lira, Felipe; Tamames, Javier; Henriques, Isabel; Martinez, Jose Luis; Manaia, Célia M.Urban wastewater treatment plants (UWTPs) are reservoirs of antibiotic resistance. Wastewater treatment changes the bacterial community and inevitably impacts the fate of antibiotic resistant bacteria and antibiotic resistance genes (ARGs). Some bacterial groups are major carriers of ARGs and hence, their elimination during wastewater treatment may contribute to increasing resistance removal efficiency. This st...
Oryzisolibacter propanilivorax gen. nov., sp nov., a propanil-degrading bacterium
Vaz-Moreira, Ivone; Narciso-da-Rocha, Carlos; Lopes, Ana Rita; Carvalho, Gilda; Lobo-da-Cunha, Alexandre; Whitman, William B.; Snauwaert, CindyStrain EPL6T, a Gram-negative, motile, short rod was isolated from a propanil and 3,4-dichloroaniline enrichment culture produced from rice paddy soil. Based on the analyses of the 16S rRNA gene sequence, strain EPL6T was observed to be a member of the family Comamonadaceae , sharing the highest pairwise identity with type strains of the species Alicycliphilus denitrificans K601T (96.8 %) and Melaminivora alkal...
The influence of the autochthonous wastewater microbiota and gene host on the f...
Narciso-da-Rocha, Carlos; Manaia, Célia M.The aim of this study was to assess the fate of invasive antibiotic resistance genes (ARG) discharged in wastewater. With this objective, antibiotic resistant bacteria (ARB) known to harbor specific ARG were inoculated in wastewater (hospital effluent, or municipal raw and treated wastewater) and in ultra-pure sterile water microcosms. Two sets of wastewater ARB isolates were used - set 1, Enterococcus faecalis...
Multidrug resistance phenotypes are widespread over different bacterial taxonom...
Narciso-da-Rocha, Carlos; Manaia, Célia M.The environment is the original and most ancient source of the antibiotic resistance determinants that threat the human health nowadays. In the environment, water is a privileged habitat and mode of dissemination of bacteria of different origins. Freshwater bodies that cross urban areas are supposed to hold a complex mixture of both human/animal origin and strictly environmental bacteria. In this study,wewere i...
Hydromonas duriensis gen. nov., sp. nov., isolated from freshwater
Vaz-Moreira, Ivone; Narciso-da-Rocha, Carlos; De Brandt, Evie; Vandamme, Peter; Ferreira, A. C. Silva; Lobo-da-Cunha, Alexandre; Nunes, Olga C.An aerobic, Gram-stain-negative rod, designated strain A2P5T , was isolated from the Douro river, in Porto, Portugal. Cells were catalase- and oxidase-positive. Growth occurred at15–30 8C, at pH 6–8 and in the presence of 1 % (w/v) NaCl. The major respiratory quinone was Q8, the genomic DNA had a G+C content of 47¡1 mol%, and phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol were amongst...
blaTEM and vanA as indicator genes of antibiotic resistance contamination in a ...
Narciso-da-Rocha, Carlos; Varela, Ana R.; Schwartz, Thomas; Nunes, Olga C.; Manaia, Celia M.Four indicator genes were monitored by quantitative PCR in hospital effluent (HE) and in the raw and treated wastewater of the municipal wastewater treatment plant receiving the hospital discharge. The indicator genes were the class 1 integrase gene intI1, to assess the capacity of bacteria to be involved in horizontal gene transfer processes; blaTEM, one of the most widespread antibiotic resistance genes in th...
Quinolone-resistant Escherichia coli isolated from birds of prey in Portugal ar...
Vredenburg, Jana; Varela, Ana Rita; Hasan, Badrul; Bertilsson, Stefan; Olsen, Björn; Narciso-da-Rocha, Carlos; Bonnedahl, Jonas; Stedt, JohanThe influence of geographic distribution and type of habitat on the molecular epidemiology of ciprofloxacin resistant Escherichia coli was investigated. Ciprofloxacin resistant E. coli from wastewater, urban water with faecal contamination and faeces of gulls, pigeons and birds of prey, from Portugal, Spain and Sweden were compared based on multilocus sequence typing (MLST) and quinolone resistance genetic dete...
Genotypic diversity and antibiotic resistance in Sphingomonadaceae isolated fro...
Narciso-da-Rocha, Carlos; Vaz-Moreira, Ivone; Manaia, Célia M.The aim of this study was to infer about the modes and extent of dispersion of Sphingomonadaceae via tap water. Sphingomonadaceae isolated from tap water samples in different places of a hospital were compared, based on intra-species genetic variability and antibiotic resistance phenotypes. These isolates were also compared with others isolated before from houses and dental chairs, served by the same municipal ...