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Aeromonas spp. in Freshwater Bodies: Antimicrobial Resistance and Biofilm Assembly

Nascimento, Maria; Rodrigues, João; Matias, Rui; Jordão, Luisa

Aeromonas spp. are environmental bacteria able to infect animals and humans. Here, we aim to evaluate the role of biofilms in Aeromonas persistence in freshwater. Aeromonas were isolated from water and biofilm samples and identified by Vitek-MS and 16S rRNA sequencing. Antibiotic susceptibility profiles were determined according to EUCAST, and a crystal violet assay was used to assess biofilm assembly. MTT and ...


Prospective observational study on the role of catheter colonization and multid...

Pinto, Miguel; Borges, Vitor; Nascimento, Maria; Martins, Filomena; Pessanha, Maria Ana; Faria, Isabel; Rodrigues, Joao; Matias, Rui; Joao Paulo, Gomes

Background: Central venous catheter (CVC)-related bloodstream infection (CRBSI) is a huge public health consern with considerable impact on mortality and health costs. The emergence of antimicrobial resistant microorganisms associated or not with CVC colonization by biofilms makes the treatment of CRBSI even more challeging. Methods:A 3-year observational study enrolling 3 tertiary hospitals located in Lisbon (...


Insights on catheter-related bloodstream infections: a prospective observationa...

Pinto, Miguel; Borges, Vítor; Nascimento, Maria; Martins, Filomena; Pessanha, Maria Ana; Faria, Isabel; Rodrigues, João; Matias, Rui; Gomes, João Paulo

Background: Central venous catheter-related bloodstream infection (CRBSI) is a huge public health concern with considerable impact on mortality and health costs. Aim: A three-year observational study enrolling three tertiary hospitals located in Lisbon, Portugal, was designed to identify the major aetiological agents of CRBSI, their ability to colonize central venous catheters and their antimicrobial resistance...


Associations between quality of life and central auditory processing in seniors...

Amaral, Ana Paula; Silva, Carla Matos, 1975-; Alves, Ana Rita; Prata, Cláudia; Fernandes, Carolina; Simões, Paula; Nascimento, Maria; Rocha, Clara

Introduction: Several health-related conditions have been reported to be important for quality of life (QoL) (Raggi et al., 2016). Central auditory processing (CAP) corresponds to the perceptual processing of the auditory information in the central nervous system (Musiek & Baran, 2007). With aging, alterations at the level of perception and detection of the sound in the central and peripheral system occur and h...


Exploring a link between healthcare associated infections, biofilms and antimic...

Bandeira, Maria; Borges, Vítor; Nascimento, Maria; Duarte, Aida; Gomes, Joao Paulo; Jordão, Luísa

HAIs are a major public health problem affecting 4 million patients per year in Europe. Here we present results of a retrospective study in wich the ability of K. pneumoniae isolates (collected during a period of 31 years) to assemble biofilms was evaluated; and an ongoing study of multispecies biofilms. In the first study, we found major differences in biofilm assembly kinetics, extracellular polymeric substan...


Prospective observational study of health care associated infections with centr...

Nascimento, Maria; Faria, Isabel; Pessanha, Maria Ana; Duarte, Aida; Martins, Filomena; Jordão, Luísa

Healthcare Associated Infection (HAI) are a major public health problem affecting 4 million patients per year in Europe and contribute increase the costs associated with healthcare. Cathether related bloodstream infections (CRBSI) are an important group of HAIs registering high incidence (87%) and mortality rates (23%) particulary in intensive care units (ICUs). In this work, an observational prospective study ...


First evidence of Schmallenberg virus introduction in Portugal, 2014

Esteves, Fernando; Mesquita, João R.; Vala, Helena; Cruz, Rita; Santos, Carla; Silva, Joana; Coelho, Ana; Poel, Wim; Nascimento, Maria

Objectives: Serra da Estrela native (SEN) breed of sheep is one of the most important in Portugal, standing responsible for the production of one of the most internationally recognized cheeses in the world, "Queijo Serra da Estrela” (protected designation of origin). One of the major risks to the sustainability of SEN dairy flocks if footrot, an infectious diseases that causes lameness, decrease in milk product...


Fostering critical thinking through peer review between cooperative learning gr...

Silva, Helena; Lopes, José; Dominguez, Caroline; Payan Carreira, Rita; Morais, Eva; Nascimento, Maria; Morais, Felicidade

Educational policies keep stressing the importance of critical thinking skills for promotion of academic success in Higher Education, to facilitate transition into the labour market and to foster livelong learning. Curricula in Higher Education Institutions gradually meet this necessity, integrating strategies foreseeing the development of critical thought in students. However, at this level, we still commonly ...


Pathogens in ornamental waters: a pilot study

Nascimento, Maria; Rodrigues, João; Reis, Lúcia; Nogueira, Isabel; Carvalho, Patricia; Brandão, João; Duarte, Aida; Jordão, Luísa

In parks, ornamental waters of easy access and populated with animals are quite attractive to children and yet might hide threats to human health. The present work focuses on the microbiota of the ornamental waters of a Lisboa park, characterized during 2015. The results show a dynamic microbiota integrating human pathogens such as Klebsiella pneumoniae, Aeromonas spp. and Enterobacter spp., and also antibiotic...


The math teacher: his school route and his teaching practices in the early career

Teixeira, Isabel; Costa, Cecília; Catarino, Paula; Nascimento, Maria

This study aims to investigate aspects of schooling and teaching practice in the first year of the teaching career of a professor of mathematics on the topic Systems of Equations for greater knowledge of the teacher. The method used was the analysis of documents, in particular your personal collection. The study shows that the teacher considered very early chose the teaching profession and the classes in the sc...

Date: 2016   |   Origin: Indagatio Didactica

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