8 documents found, page 1 of 1

Evaluation of partial thromboplastin time, thrombin time and prothrombin time o...

Fibrinolytic proteases are enzymes that degrade the fibrin, a main component of the blood clot. Thus, the present study aimed to understand the evaluation of aPTT, TT and PT on plasma treated with a fibrinolytic protease produced by Mucor subtillissimus. To assess the anticoagulant effect, the protease was used in different concentrations of 0.5-2.5mg/mL. The tests showed that the enzyme promoted a significant ...

Protease with fibrinolytic and collagenolytic activity produced by Aspergillus ...

Proteases play a fundamental role in the economy, being present in several industrial sectors. Among the fungi known to produce proteases, the genus Aspergillus stands out. Thus, the objective of this work was to produce proteases from solid state fermentation of Aspergillus ochraceus, purify, characterize and evaluate their potential in the production of collagenolytic and fibrinolytic enzymes. For this, the t...

Purification of a lectin from Cratylia mollis crude extract seed by a single st...

The partitioning and purification of lectins from the crude extract of Cratylia mollis seeds (Cramoll 1,4) was investigated in aqueous two-phase systems (ATPS). A factorial design model (24) was used to evaluate the influence of polyethylene glycol (PEG) molar mass (15008000g/mol), PEG concentration (12.517.5% w/w), phosphate (1015% w/w) concentration, and pH (68) on the differential partitioning, purification ...

Simultaneous production and recovery in situ of fibrinolytic protease from Muco...

Purification of a fibrinolytic protease from Mucor subtilissimus UCP 1262 by aq...

A fibrinolytic protease from M. subtilissimus UCP 1262 was recovered and partially purified by polyethylene glycol (PEG)/sodium sulfate aqueous two-phase systems (ATPS). The simultaneous influence of PEG molar mass, PEG concentration and sulfate concentration on the enzyme recovery was first investigated using a 23 full factorial design, and the Response Surface Methodology used to identify the optimum conditio...

Produção de proteases com atividade fibrinolítica por fungos filamentosos de so...

Enzimas fibrinolíticas têm recebido atenção, devido ao seu potencial medicinal para doenças trombolíticas, estas estão se tornando uma das principais causas de morbidade e mortalidade em todo o mundo. Este trabalho teve como finalidade obter condições de produção e caracterização de proteases fibrinolítica, produzida por fungos filamentosos isolados de solo da Caatinga-PE-Brasil, utilizando fermentação em estad...

Production and characterization of new fibrinolytic protease from Mucor subtill...

Fibrinolytic enzymes have received attention regarding their medicinal potential for thrombolytic diseases, a leading cause of morbidity and mortality worldwide. Various natural enzymes purified from animal, plant and microbial sources have been extensively studied. The aim of this work was to produce fibrinolytic protease by solid state fermentation using agro industrial substrates. Rhizopus arrhizus var. arrh...

Evaluation the best condition of Fibrinolytic protease production using factori...

Fibrinolytic enzymes have the ability to degrade fibrin clots formed for avoiding intravascular thrombosis. In the pharmaceutical industry there is a search for new fibrinolytic agent that reduces the production cost and increasing productivity. The use of microorganism for enzyme production, such as the genus Streptomyces has been reported. Streptomyces is a Gram-positive bacteria, responsible for producing ma...