9 documents found, page 1 of 1
The maintenance of centriole appendages and motile cilia basal body anchoring r...
Carmona, Bruno; Camelo, Carolina; Mehraz, Manon; Lemullois, Michel; Faria, Mariana Lince; Coyaud, Étienne; Marinho, H. Susana; Gonçalves, JoãoCentrosomes are organelles consisting of two structurally and functionally distinct centrioles, with the mother centriole having complex distal (DA) and subdistal appendages (SDA). Despite their importance, how appendages are assembled and maintained remains unclear. This study investigated human TBCCD1, a centrosomal protein essential for centrosome positioning, to uncover its localization and role at centriol...
From centrosomal microtubule anchoring and organization to basal body positioni...
Carmona, Bruno; Camelo, Carolina; Mehraz, Manon; Lemullois, Michel; Ferreira, David C.; Nolasco, Sofia; Lince-Faria, Mariana; Marino, H. SusanaCilia are microtubule-based organelles that protrude from the cell surface and fulfill critical motility and sensory functions being required for normal embryonic development and for homeostasis of human adult tissues. Cilia loss or dysfunction is associated with human ciliopathies. At their base cilia have a centriole/basal body (BB), which can be derived from the centrosome and assembles the ciliary axoneme. ...
TBCCD1: a new player in the development of ciliopathies?
Carmona, Bruno; Camelo, Carolina; Mehraz, Manon; Lemullois, Michel; Ferreira, David C.; Nolasco, Sofia; Lince-Faria, Mariana; Marinho, H. SusanaCilia are hair-like appendages, consisting of a microtubule (MT)-based ciliary axoneme, which fulfill critical motility and sensory functions required for normal embryonic development and also for homeostasis of adult tissues. At their base, cilia have a centriole/basal body, which can be derived from the centrosome, and that nucleates the ciliary axoneme. Centrosomes consist of a pair of centrioles surrounded ...
Centrosome positioning and development of ciliopathies: role of the human centr...
Carmona, Bruno; Camelo, Carolina; Mehraz, Manon; Lemullois, Michel; Ferreira, David C.; Nolasco, Sofia; Lince-Faria, Mariana; Marinho, H. SusanaAims/Context: Primary cilia are specialized microtubule-based signaling organelles that convey extracellular signaling and cellular polarity into a cellular response. Defects in primary cilia assembly/function cause severe diseases known as ciliopathies, typified by clinical manifestations, like infertility, obesity, brain problems, blindness, and kidney cysts. Primary cilia assembly entails centrosome migratio...
TBCCD1 is a key regulator of centrosomal microtubule anchor and basal body posi...
Carmona, Bruno; Camelo, Carolina; Mehraz, Manon; Lemullois, Michel; Nolasco, Sofia; Marinho, H. Susana; Tassin, Anne-Marie; Koll, France; Soares, HelenaSuccessful cilia assembly requires correct positioning and anchoring of the centrosome's mother centriole/basal body (BB) to the cell membrane. A clear picture of the different signals and players involved in centrosome positioning/anchoring is still not available. Published work from our group identified a new TBCC domain-containing human protein (TBCCD1). Depletion of TBCCD1 in human RPE-1 cells severely affe...
Opi1p translocation to the nucleus is regulated by hydrogen peroxide in Sacchar...
Camelo, Carolina; Vilas-Boas, Filipe; Cepeda, Andreia Pereira; Real, Carla; Barros-Martins, Joana; Pinto, Francisco; Soares, Helena; Marinho, H. SusanaDuring exposure of yeast cells to low levels of hydrogen peroxide (H2 O2 ), the expression of several genes is regulated for cells to adapt to the surrounding oxidative environment. Such adaptation involves modification of plasma membrane lipid composition, reorganization of ergosterol-rich microdomains and altered gene expression of proteins involved in lipid and vesicle traffic, to decrease permeability to ex...
TBCCD1 and OFD1, a novel centrosomal protein partnership with a role in human c...
Veiga, Joaquim; Carmona, Bruno; Camelo, Carolina; Pereira, Dulce; Marinho, H. Susana; Soares, HelenaCilia are hair-like appendages, consisting of a microtubule (MT)-based ciliary axoneme, that fulfill critical motility and sensory functions required for normal embryonic development and also for homeostasis of adult tissues. At their base, cilia have a centriole/basal body, which can be derived from the centrosome, and that nucleates the ciliary axoneme (primary cilia). Centrosomes consist of a pair of centrio...
Unraveling the role of TBCCD1 protein on cell size control: the regulation of c...
Camelo, Carolina; Peneda, Catarina; Coyaud, Étienne; Raught, Brian; Câmara, Ana I.; Carmona, Bruno; Pinto, Francisco; Narinho, H. Susana; Soares, HelenaDuring their lifetime most cells maintain their size. There is increasing evidence showing that this process may be dynamic and that cells can adapt their size in response to external signals and changes in the environment [1], which strongly suggests that cell size is regulated. Both Hippo and IGF/PI3K/AKT/mTORC1 pathways have been described as being involved in cell size/growth control [1]. Interestingly, the...
OPI1P translocation to the nucleus is regulated through oxidation by hydrogen p...
Camelo, Carolina; Vilas-Boas, F.; Cepeda, A.; Real, C.; Pinto, F.; Costa, G. da; Soares, Helena; Marinho, H. Susana; Cyrne, L.Adaptation of yeast cells to hydrogen peroxide (H2O2) leads to a rapid change in membrane permeability accompanied by a decrease of membrane fluidity and the alteration of its lipid composition, allowing cells to survive to higher doses of H2O2. During adaptation to H2O2 several genes that contain the regulatory element UASINO, and which codify for enzymes involved in phospholipid and fatty acid metabolism, are...