44 documents found, page 1 of 5
Propolis potential activity against Candida tropicalis adhered cells and its bi...
Tobaldini, F. K.; Bonfim-Mendonça, P. S.; Rosseto, H. C.; Bruschi, M. L.; Henriques, Mariana; Azeredo, Joana; Negri, M.; Silva, Sónia CarinaObjectives: Invasive fungal infections, such as candidiasis, represent a public health problem of major importance, and Candida tropicalis has been highlighted among the main agents of candidiasis. One of the major contributions to C. tropicalis virulence is its versatility in adapting to a variety of different habitats and the formation of surface attached microbial communities known as biofilms. Moreover, fro...
Genotypic variability and antifungal susceptibility of Candida spp. isolated fr...
Sakita, K. M.; Faria, D. R.; Tobaldini, F. K.; Bettega, E. M. S.; Negri, M.; Kioshima, E. S.; Svidzinski, T. I. E.; Bonfim-Mendonça, P. S.Objectives Candida spp. are responsible for 9095% of hematogenous fungal infections. In Brazil and Latin America, C. albicans is the most common specie, followed by C. parapsilosis and C. tropicalis. Infections caused by Candida spp. may have their origin in exogenous sources, transmitted to patients via contaminated infusions, biomedical devices or even by the hands of the hospital staff members. Molecular bio...
Assessment of in vitro biofilm formation and antifungal susceptibility of Candi...
Faria, D. R.; Sakita, K. M.; Tobaldini, F. K.; Akimoto-Gunther, L.; Negri, M.; Kioshima, E. S.; Svidzinski, T. I. E.; Bonfim-Mendonça, P. S.Objectives Vulvovaginal candidiasis (VVC) is an inflammation of the genital mucosa, which mainly affects the vulva and vagina. Candida spp. are considered commensal fungus, however, when there is imbalance in the microbiota or the host immune system is compromised, these can become pathogenic. C. albicans is responsible for most cases of VVC and is able of expressing mechanisms which allow the colonization or i...
Evaluation of propolis and its subproduct as an inhibitor of growth and biofilm...
Moraes, L. B.; Salci, T. P.; Bonfim-Mendonça, P. S.; Tobaldini, F. K.; Toledo, L. A. S.; Negri, M.; Bruschi, M. L.; Svidzinski, T. I. E.Objectives The treatment of vulvovaginal candidiasis (VVC) is still unsatisfactory, especially in pregnant women, being promising to the utilization of alternative therapies. Propolis extract solution (PES) has demonstrated antifungal efficacy and low toxicity. In addition, the subproduct of propolis extract solution (SPES) is produced during the process of preparing PES and is usually discarded, but can still ...
Influence of Laminarin in colonisation process of Candida albicans
Bonfim-Mendonça, P. S.; Tobaldini, F. K.; Batalini, I. M.; Capoci, I. R. G.; Godoy, J. S. R.; Kioshima, E. S.; Negri, M.; Svidzinski, T. I. E.Objective Candida albicans is responsible for the majority of cases of vulvovaginal candidiasis (VVC), one of the most important candidal virulence factors is the ability to adhere to host surfaces. Chemotherapies that seek to improve the host immune response are an alterna- tive to control fungal infections. b-glucans are polymeric carbohydrates that have been reported to modulate human inflammatory responses ...
Adhesion of Candida biofilm cells to human epithelial cells and polystyrene aft...
Monteiro, D. R.; Negri, M.; Silva, Sónia Carina; Gorup, L. F.; Camargo, E. R.; Oliveira, Rosário; Barbosa, D. B.; Henriques, MarianaThis study investigated the adhesion to human epithelial cells and polystyrene surface of viable yeasts recovered from Candida biofilms treated with silver nanoparticles (SN). Biofilm resuspended Candida cells were added to HeLa cells or to empty wells of microtiter plates and the adhesion was verified using crystal violet staining. The adhesion of Candida cells was significantly reduced, mainly when biofilms w...
Silver colloidal nanoparticles : effect on matrix composition and structure of ...
Monteiro, D. R.; Silva, Sónia Carina; Negri, M.; Gorup, L. F.; Camargo, E. R.; Oliveira, Rosário; Barbosa, D. B.; Henriques, MarianaAim : The aim of this study was to assess the effect of different silver nanoparticles (SN) concentrations on the matrix composition and structure of Candida albicans and Candida glabrata biofilms. Methods and Results : Candida biofilms were developed in 6-well microtiter plates during 48 h. After, these biofilms were exposed to 13·5 or 54 μg SN ml−1 for 24 h. Then, extracellular matrices were extracted from bi...
Virulence factor of Candida tropicalis isolated from hospitalized patients
Negri, M.; Henriques, Mariana; Svidzinski, T.; Azeredo, Joana; Oliveira, RosárioCandida tropicalis has been reported to be one of the Candida species which is most likely to cause bloodstream and urinary tract infections in hospitals. Several virulence factors seem to be responsible for C. tropicalis infections, which present high potential for dissemination and mortality. The aim of this study was to investigate the correlation between different virulence factors (enzymes secretion, adhes...
Antifungal activity of silver nanoparticles in combination with nystatin and ch...
Monteiro, D. R.; Silva, Sónia Carina; Negri, M.; Gorup, L. F.; Camargo, E. R.; Oliveira, Rosário; Barbosa, D. B.; Henriques, MarianaAlthough silver nanoparticles (SN) have been investigated as an alternative to conventional antifungal drugs in the control of Candida-associated denture stomatitis, the antifungal activity of SN in combination with antifungal drugs against Candida biofilms remains unknown. Therefore, the aim of this study was to evaluate the antifungal efficacy of SN in combination with nystatin (NYT) or chlorhexidine diglucon...
The effect of silver nanoparticles and nystatin on mixed biofilms of Candida gl...
Silva, Sónia Carina; Pires, Diana Priscila Penso; Henriques, Mariana; Monteiro, D. R.; Negri, M.; Gorup, L. F.; Camargo, E. R.; Barbosa, D. B.The purpose of this study was to examine the effect of silver nanoparticles (SN) and nystatin on pre-formed biofilms (single and dual species combinations of Candida glabrata and Candida albicans). Thus, adhesion and biofilm assays were performed on acrylic surface in presence of artificial saliva (AS), during 2 h and 48 h, respectively. Candida glabrata and C. albicans adhered cells were determined by counting...