7 documents found, page 1 of 1
Standardization of resazurin use in susceptibility testing of natural products ...
Barros, Isabella Letícia Esteves; Veiga, Flávia Franco; Corrêa, Jakeline Luiz; Jarros, Isabele Carrilho; Negri, MelyssaNatural products, such as the ethanolic propolis extract (PE), have been shown to be a safe and effective therapeutic alternative for the treatment of fungal skin and nail diseases. However, the presence of the resin and the physicochemical characteristics of the extract sometimes difficult the reading and determination of breakpoints of the in vitro tests, evidencing the need for alternatives that facilitate t...
Antibacterial and antibiofilm activity of carvacrol against Salmonella enterica...
Trevisan, Daliah Alves Coelho; Silva, Alex Fiori da; Negri, Melyssa; Abreu Filho, Benício Alves de; Machinski Junior, Miguel; Patussi, Eliana ValériaThe present study evaluated the antibacterial and antibiofilm activity of carvacrol against Salmonella Typhimurium. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined and the time-kill curve and scanning electron microscopy (SEM) were performed to evaluate antibacterial activity. Antibiofilm activity was evaluated by quantifying total biomass using crystal vi...
Virulence factors and genetic variability of vaginal Candida albicans isolates ...
Zanni, Pâmela Cristina Mastellaro Delvas; Bonfim-Mendonça, Patrícia de Souza; Negri, Melyssa; Nakamura, Sandra Sayuri; Donatti, LucéliaVulvovaginal candidiasis (VVC) in HIV-infected (HIV+) women is a serious public health problem. However, little is known about the virulence mechanisms of vaginal Candida albicans from HIV+ women in the post-highly active antiretroviral therapy (HAART) era. Here, we report a comparative analysis of the expression of key virulence factors and genetic variability of 26 vaginal C. albicans strains isolated from HI...
Antibiofillm activity of propolis extract on Fusarium species from onychomycosis
Galletti, Juliana; Tobaldini-Valerio, Flávia; Silva, Sónia Carina; Kioshima, Erika Seki; Trierveiler-Pereira, Larissa; Bruschi, Marcos; Negri, MelyssaAim: The present study evaluated the capacity of three species of Fusarium isolated from onychomycosis to form bio lms and the antibio lm effect of propolis extract on these bio lms. Materials & methods: The bio lms and antibio lm effects were evaluated by quantifying the colony-forming units, mitochondrial metabolic activity assays, total biomass by crystal violet staining and scanning electron microscopy. Res...
Propolis: a potential natural product to fight Candida species infections
Tobaldini-Valerio, Flávia; Bonfim-Mendonça, P. S.; Rosseto, H. C.; Bruschi, M. L.; Henriques, Mariana; Negri, Melyssa; Silva, Sónia CarinaAim: To evaluate the effect of propolis against Candida species planktonic cells and its counterpart's biofilms. Materials & methods: The MIC values, time-kill curves and filamentation form inhibition were determined in Candida planktonic cells. The effect of propolis on Candida biofilms was assessed through quantification of CFUs. Results: MIC values, ranging from 220 to 880 µg/ml, demonstrated higher efficien...
Silver nanoparticles to fight Candida coinfection in the oral cavity
Monteiro, Douglas Roberto; Silva, Sónia Carina; Negri, Melyssa; Gorup, Luiz Fernando; Camargo, Emerson Rodrigues de; Barbosa, Debora Barros[Exert] This chapter is focused on the activity of silver nanoparticles (SN) as an antifungal agent against Candida albicans and Candida glabrata biofilms, which are involved in oral candidosis. A discussion focusing on the influence of the stabilizing agent, diameter of SN on its antibiofilm activity, influence of chemical stability of SN on Candida biofilms, the effect of SN against adhered cells and biofilms...
Candida tropicalis biofilms : artificial urine, urinary catheters and flow model
Negri, Melyssa; Silva, Sónia Carina; Henriques, Mariana; Azeredo, Joana; Svidzinski, Terezinha; Oliveira, RosárioAdhesion to medical devices and biofilm formation are considered important virulence factors of Candida tropicalis. This work aimed to use artificial urine (AU) and urinary catheters, under flow conditions, for the study of C. tropicalis biofilms. Adhesion and biofilm formation on silicone and latex urinary catheters was quantified by crystal violet staining and colony forming units determination. Candida surfa...