14 documents found, page 1 of 2
Planting sustainability: A comprehensive review of plant fibres in needle-punch...
Marques, Rita; Oliveira, Cristina; Araújo, Joana Carolina Carvalho; Chaves, Diego Morais; Ferreira, Diana P.; Fangueiro, Raúl; Silva, Carla J.Natural fibres have garnered substantial attention because of their eco-friendly attributes and versatility, offering a sustainable alternative to synthetic ones. This review surveys plant fibres, including flax, hemp, jute, banana, and pineapple, emphasizing their diverse properties and applications in nonwoven materials. This research also examines the use of synthetic polymer composites blended with natural ...
Electrochemical aptasensor for the detection of the key virulence factor YadA o...
Sande, Maria Georgina; Ferreira, Débora Carina Gonçalves Abreu; Rodrigues, Joana Lúcia Lima Correia; Melo, Luís Daniel Rodrigues; Linke, DirkNew point-of-care (POC) diagnosis of bacterial infections are imperative to overcome the deficiencies of conventional methods, such as culture and molecular methods. In this study, we identified new aptamers that bind to the virulence factor Yersinia adhesin A (YadA) of Yersinia enterocolitica using cell-systematic evolution of ligands by exponential enrichment (cell-SELEX). Escherichia coli expressing YadA on ...
Electrochemical Aptasensor for the Detection of the Key Virulence Factor YadA o...
Sande, Maria Georgina; Ferreira, Débora; Rodrigues, Joana; Melo, Luís; Linke, Dirk; Silva, Carla J.; Moreira, Felismina; Sales, Goreti; Rodrigues, LígiaNew point-of-care (POC) diagnosis of bacterial infections are imperative to overcome the deficiencies of conventional methods, such as culture and molecular methods. In this study, we identified new aptamers that bind to the virulence factor Yersinia adhesin A (YadA) of Yersinia enterocolitica using cell-systematic evolution of ligands by exponential enrichment (cell-SELEX). Escherichia coli expressing YadA on ...
Collagen-coated magnetic nanoparticles to capture pathogens
Sande, Maria; Roque, Lúcia; Marques, Márcia; Ferreira, Débora; Rodrigues, Joana Lúcia Lima Correia; Braga, Adelaide Correia; Ramada, DiogoBackground The conventional method of diagnosing bacterial infections is based on microbial culture, however this method can take several hours or even days and lacks sensitivity. In the past few decades, the use of immunological and molecular techniques such as ELISA, PCR and mass spectrometry are used to diagnose infections. While these methods can be highly sensitive and reliable, they often require sample p...
DNA aptamers towards Yersinia enterocolitica and Moraxella catarrhalis adhesins...
Sande, Maria Georgina; Ferreira, Débora Carina Gonçalves Abreu; Rodrigues, Joana Lúcia Lima Correia; Melo, Luís Daniel Rodrigues; Silva, Carla J.info:eu-repo/semantics/publishedVersion
Novel biorecognition elements against pathogens in the design of state-of-the-a...
Sande, Maria Georgina; Rodrigues, Joana Lúcia Lima Correia; Ferreira, Débora Carina Gonçalves Abreu; Silva, Carla J.; Rodrigues, L. R.Infectious agents, especially bacteria and viruses, account for a vast number of hospitalisations and mortality worldwide. Providing effective and timely diagnostics for the multiplicity of infectious diseases is challenging. Conventional diagnostic solutions, although technologically advanced, are highly complex and often inaccessible in resource-limited settings. An alternative strategy involves convenient ra...
Selection and validation of DNA aptamers against Yersinia Enterocolitica and Mo...
Sande, Maria Georgina; Ferreira, Débora; Rodrigues, Joana Lúcia Lima Correia; Melo, Luís Daniel Rodrigues; Silva, Carla J.; Rodrigues, Lígia R.info:eu-repo/semantics/publishedVersion
Modulating cell adhesion to polybutylene succinate biotextile constructs for ti...
Ribeiro, Viviana Pinto; Almeida, Lília Rosa Santos Pinto; Martins, Ana Regina Ferreira; Pashkuleva, I.; Marques, A. P.; Ribeiro, Ana S.; Silva, Carla J.Textile-based technologies are powerful routes for the production of three-dimensional porous architectures for tissue engineer- ing applications because of their feasibility and possibility for scaling-up. Herein, the use of knitting technology to produce polybutylene succinate fibre-based porous architectures is described. Furthermore, different treatments have been applied to functionalize the surface of the...
Antimicrobial enzyme immobilization in bacterial cellulose
Sampaio, Liliana M. P.; Ribeiro, Jorge Manuel Padrão; Faria, Jorge; Silva, João Pedro Martins Soares Castro; Silva, Carla J.; Dourado, FernandoThis work studied the physical immobilization of laccase on bacterial nanocellulose (BNC) aiming to identify the enzyme antibacterial properties suitable for wound dressings. The pH optimum and activation energy of free laccase depends on the substrate employed. The Michaelis-Menten constant for the immobilized laccase was found to be almost double of that of the free enzyme. However, the specific activities of...
Silk-based anisotropical 3D biotextiles for bone regeneration
Ribeiro, Viviana P.; Silva-Correia, Joana; Nascimento, Ana I.; Morais, Alain da Silva; Marques, Alexandra P.; Ribeiro, Ana S.; Silva, Carla J.Bone loss in the craniofacial complex can been treated using several conventional therapeutic strategies that face many obstacles and limitations. In this work, novel three-dimensional (3D) biotextile architectures were developed as a possible strategy for flat bone regeneration applications. As a fully automated processing route, this strategy as potential to be easily industrialized. Silk fibroin (SF) yarns w...