2 documents found, page 1 of 1
Quality Control of Biotechnological Inputs DetectingMycoplasma
Netto,Cristiane; Soccol,Vanete Thomaz; Sepulveda,Lya; Garcia,Gabriel Henrique Oliveira; Timenetsky,JorgeThe aim of this work was to study the Polymerase Chain Reaction (PCR) as a tool of quality control of bovine sera and cellular cultures used in the biotechnological industry. A total of 46 samples of bovine sera derived from two slaughterhouses and 33 samples of BHK21 cells derived from two biotechnological industries were evaluated using the primers GPO-3 (sense) and MGSO (antisense). The PCR technique sensibi...
Experimental infection of BHK21 and Vero cell lines with different Mycoplasma spp.
Netto,Cristiane; Soccol,Vanete Thomaz; Sepulveda,Lya Madureira; Timenetsky,JorgeMycoplasma spp, belongs to the class Mollicutes and is capable to produce alterations in cellular cultures causing damages to the biotechnological industry. Bioproducts generally require two essential inputs, bovine serum and cells. The study herein aims to evaluate the mycoplasma concentrations that affect the growing of BHK21 and Vero cells. The species used were: Mycoplasma orale, M. salivarium, M. arginini ...