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The influence of P. fluorescens cell morphology on the lytic performance and pr...

Sillankorva, Sanna; Pires, Diana Priscila Penso; Oliveira, Hugo Alexandre Mendes; Neubauer, P.; Azeredo, Joana

This study aims at assessing the influence of Pseudomonas fluorescence cell morphology on the effectiveness and production of the lytic bacteriophage /IBBPF7A. P. fluorescens were cultured as rods or as elongated cells by varying the temperature and rotary agitation conditions. Cells presented rod shape when grown at temperatures up to 25C and also at 30C under static conditions, and elongated morphology only a...


Complete genome sequence of the lytic Pseudomonas fluorescens phage ϕIBB-PF7A

Sillankorva, Sanna; Kluskens, Leon; Linghorn, E.; Kropinski, Andrew M.; Neubauer, P.; Azeredo, Joana

Background Phage ϕIBB-PF7A is a T7-like bacteriophage capable of infecting several Pseudomonas fluorescens dairy isolates and is extremely efficient in lysing this bacterium even when growing in biofilms attached to surfaces. This work describes the complete genome sequence of this phage. Results The genome consists of a linear double-stranded DNA of 40,973 bp, with 985 bp long direct terminal repeats and a GC ...


Phage control of dual species biofilms of Pseudomonas fluorescens and Staphyloc...

Sillankorva, Sanna; Neubauer, P.; Azeredo, Joana

Despite the recent enthusiasm for using bacteriophages as bacterial control agents, there are only limited studies concerning phage interaction with their respective hosts residing in mixed biofilm consortia and especially in biofilms where the host species is a minor constituent. In the present work, a study was made of mono and dual species biofilms formed by Pseudomonas fluorescens (Gram-negative) and/or Sta...


Dual species biofilms and their control with bacteriophages

Sillankorva, Sanna; Neubauer, P.; Azeredo, Joana


Isolation and characterization of a T7-like lytic phage for Pseudomonas fluores...

Sillankorva, Sanna; Neubauer, P.; Azeredo, Joana

Background: Despite the proven relevance of Pseudomonas fluorescens as a spoilage microorganism in milk, fresh meats and refrigerated food products and the recognized potential of bacteriophages as sanitation agents, so far no phages specific for P. fluorescens isolates from dairy industry have been closely characterized in view of their lytic efficiency. Here we describe the isolation and characterization of a...


Pseudomonas fluorescens biofilms subjected to phage phiIBB-PF7A

Sillankorva, Sanna; Neubauer, P.; Azeredo, Joana

Background: Pseudomonas fluorescens is an important food spoilage organism, usually found in the form of biofilms. Bacterial biofilms are inherently resistant to a variety of antimicrobial agents, therefore alternative methods to biofilm control, such as bacteriophages (phages) have been suggested. Phage behavior on biofilms is still poorly investigated and needs further understanding. Here we describe the appl...


Phage infection of dual species biofilms

Sillankorva, Sanna; Neubauer, P.; Azeredo, Joana


Biofilm control with T7 phages

Sillankorva, Sanna; Pospiech, H.; Azeredo, Joana; Neubauer, P.

Fundação para a Ciência e a Tecnologia (FCT)


Biofilm control with T7 phages

Sillankorva, Sanna; Pospiech, H.; Azeredo, Joana; Neubauer, P.

Fundação para a Ciência e a Tecnologia (FCT).


T7-like bacteriophages to control biofilm forming bacteria

Sillankorva, Sanna; Pospiech, H.; Azeredo, Joana; Neubauer, P.

Fundação para a Ciência e a Tecnologia (FCT)


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