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MiDAS 5: Global diversity of bacteria and archaea in anaerobic digesters

Dueholm, Morten Kam Dahl; Andersen, Kasper Skytte; Korntved, Anne-Kirstine C.; Rudkjøbing, Vibeke; Alves, M. M.; Bajón-Fernández, Yadira

Anaerobic digestion of organic waste into methane and carbon dioxide (biogas) is carried out by complex microbial communities. Here, we use full-length 16S rRNA gene sequencing of 285 full-scale anaerobic digesters (ADs) to expand our knowledge about diversity and function of the bacteria and archaea in ADs worldwide. The sequences are processed into full-length 16S rRNA amplicon sequence variants (FL-ASVs) and...


MiDAS 5: Global diversity of bacteria and archaea in anaerobic digesters

Dueholm, Morten Kam Dahl; Andersen, Kasper Skytte; Petersen, Anne-Kirstine C.; Rudkjøbing, Vibeke; Alves, M. M.; Bajón-Fernández, Yadira

Anaerobic digestion represents a key biotechnology for the transformation of organic waste into renewable energy (biogas) and relies on complex microbial communities that work in concert to degrade the complex substrates into methane and carbon dioxide. Here, we sequenced more than half a million high-quality, full-length 16S rRNA gene sequences from 285 full-scale anaerobic digesters (ADs) across the world to ...


Candidate biomarkers of antibiotic resistance for the monitoring of wastewater ...

Teixeira, A. Margarida; Vaz-Moreira, Ivone; Calderón-Franco, David; Weissbrodt, David; Purkrtova, Sabina; Gajdos, Stanislav; Dottorini, Giulia

Urban wastewater treatment plants (UWTPs) are essential for reducing the pollutants load and protecting water bodies. However, wastewater catchment areas and UWTPs emit continuously antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs), with recognized impacts on the downstream environments. Recently, the European Commission recommended to monitor antibiotic resistance in UWTPs serving more...


Biomarkers for monitoring antibiotic resistance in aquatic environments

Teixeira, Ana Margarida Ribeiro; Vaz-Moreira, Ivone; Calderón-Franco, David; Weissbrodt, David Gregory; Purkrtova, Sabina; Gajdos, S.; Dottorini, G.

The occurrence of antimicrobial resistance raises concerns as a human health threat that can be propagated through the environment. Wastewater discharge into the environment is an important source for antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs). Sewage collection and urban wastewater treatment plants (UWTPs) are major barriers that reduce environmental contamination by sewage-deri...


Biomarkers for monitoring antibiotic resistance in aquatic environments

Teixeira, A. Margarida; Vaz-Moreira, Ivone; Franco, David Calderón; Weissbrodt, David; Purkrtova, Sabina; Gajdos, Stanislav; Dottorini, Giulia


Monitoring antibiotic resistance genes in wastewater environments: the challeng...

Miłobedzka, Aleksandra; Ferreira, Catarina; Vaz-Moreira, Ivone; Calderón-Franco, David; Gorecki, Adrian; Purkrtova, Sabina; Bartacek, Jan

Antibiotic resistance (AR) is a global problem requiring international cooperation and coordinated action. Global monitoring must rely on methods available and comparable across nations to quantify AR occurrence and identify sources and reservoirs, as well as paths of AR dissemination. Numerous analytical tools that are gaining relevance in microbiology, have the potential to be applied to AR research. This rev...


Monitoring antibiotic resistance in aquatic environments

Teixeira, A. Margarida; Cachetas, Diogo; Vaz-Moreira, Ivone; Rocha, Jaqueline; Franco, David Calderón; Weissbrodt, David; Purkrtova, Sabina


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