11 documents found, page 1 of 2
A double-antibody sandwich ELISA based on the porcine circovirus type 2 (PCV2) ...
Cruz,Taís F.; Kanashiro,Tatiana M.; Castro,Alessandra M.M.G. de; Baldin,Cintia M.; Richtzenhain,Leonardo J.; Araujo Jr,João P.ABSTRACT: Few studies have described enzyme-linked immunosorbent assays (ELISAs) for the detection of antibodies against porcine circovirus type 2 (PCV2) based on antigens produced in cell culture. Furthermore, few articles have described viral purification techniques for members of the family Circoviridae. This occurs because circoviruses are difficult to isolate, noncytopathogenic, and produce low viral titre...
Molecular characterization of Brazilian equid herpesvirus type 1 strains based ...
Mori,Enio; Lara,Maria do Carmo C.S.H.; Cunha,Elenice M.S.; Villalobos,Eliana M.C.; Mori,Claudia M.C.; Soares,Rodrigo M.; Brandão,Paulo E.Partial nucleotide sequences of ORF72 (glycoprotein D, gD), ORF64 (infected cell protein 4, ICP4) and ORF30 (DNA polymerase) genes were compared with corresponding sequences of EHV-1 reference strains to characterize the molecular variability of Brazilian strains. Virus isolation assays were applied to 74 samples including visceral tissue, total blood, cerebrospinal fluid (CSF) and nasal swabs of specimens from...
Análise filogenética de isolados do vírus da raiva de herbívoros na fronteira d...
Estévez Garcia,Andrea I.; Peixoto,Haila C.; Silva,Sheila O.; Polo,Gina; Alves,Ana J.; Brandão,Paulo E.; Cunha,Elenice M.; Richtzenhain,Leonardo J.A raiva transmitida por morcegos hematófagos da espécie Desmodus rotundus representa uma preocupação de saúde pública e causa de importantes prejuízos para a pecuária brasileira. A evidência atual sugere que a ocorrência de raiva está relacionada às características da paisagem, topografia, hidrografia, sistemas de produção animal e usos da terra. Contudo, existem poucos estudos que analisem as possíveis conexõe...
Canine distemper virus infection in a lesser grison (Galictis cuja): first repo...
Megid,Jane; Teixeira,Carlos R.; Cortez,Adriana; Heinemann,Marcos B.; Antunes,João M.A.P.; Fornazari,Felipe; Rassy,Fabricio B.; Richtzenhain,Leonardo J.Infectious diseases in wild animals have been increasing as a result of their habitat alterations and closer contact with domestic animals. Canine distemper virus (CDV) has been reported in several species of wild carnivores, presenting a threat to wildlife conservation. We described the first case of canine distemper virus infection in lesser grison (Galictis cuja). A free-ranging individual, with no visible c...
Rickettsia felis infection in cat fleas Ctenocephalides felis felis
Horta,Mauricio C.; Scott,Fabio B.; Correia,Thaís R.; Fernandes,Julio I.; Richtzenhain,Leonardo J.; Labruna,Marcelo B.The present study evaluated the rickettsial infection in a laboratory colony of cat fleas, Ctenocephalides felis felis (Bouche) in Brazil. All flea samples (30 eggs, 30 larvae, 30 cocoons, 30 males, and 30 females) tested by polymerase chain reaction (PCR) were shown to contain rickettsial DNA. PCR products, corresponding to the rickettsial gltA, htrA, ompA and ompB gene partial sequences were sequenced and sho...
Rapid detection of bovine coronavirus by a semi-nested RT-PCR
Asano,Karen M.; Souza,Sibele P.; Silva,Sheila O.S.; Richtzenhain,Leonardo J.; Brandão,Paulo E.Bovine coronavirus (BCoV) is a member of the group 2 of the Coronavirus (Nidovirales: Coronaviridae) and the causative agent of enteritis in both calves and adult bovine, as well as respiratory disease in calves. The present study aimed to develop a semi-nested RT-PCR for the detection of BCoV based on representative up-to-date sequences of the nucleocapsid gene, a conserved region of coronavirus genome. Three ...
Ehrlichia canis em cães atendidos em hospital veterinário de Botucatu, Estado d...
Ueno,Tatiana E. H.; Aguiar,Daniel M.; Pacheco,Richard C.; Richtzenhain,Leonardo J.; Ribeiro,Márcio G.; Paes,Antônio C.; Megid,Jane; Labruna,Marcelo B.O presente estudo investigou a etiologia da erliquiose monocítica canina em 70 cães atendidos no Hospital Veterinário da Universidade Estadual Paulista, na cidade de Botucatu, durante 2001 e 2002. Os cães foram avaliados segundo achados clínicos, epidemiológicos e laboratoriais e pela amplificação parcial e sequenciamento do gene dsb de Ehrlichia. DNA de Ehrlichia canis foi amplificado e sequenciado em 28 (40,0...
Genetic grouping of avian infectious bronchitis virus isolated in Brazil based ...
Montassier,Maria de Fátima S.; Brentano,Liana; Montassier,Hélio J.; Richtzenhain,Leonardo J.Twelve Brazilian isolates and one reference vaccine strain of avian infectious bronchitis virus (IBV) were propagated in embryonating chicken eggs. The entire S1 glycoprotein gene of these viruses was analysed by reverse-transcriptase-polymerase chain reaction and restriction fragment length polymorphism (RT-PCR-RFLP), using the restriction enzymes HaeIII, XcmI and BstyI. The RFLP patterns led to the classifica...
On the etiology of an outbreak of winter dysentery in dairy cows in Brazil
Brandão,Paulo E.; Villarreal,Laura Y.B.; Gregori,F.; Souza,Silvio L.P. de; Lopes,Marco A.E.; Gomes,Cleise R.; Sforsin,Angelo J.; Sanches,Alexandre A.Winter dysentery (WD) is a seasonal infectious disease described worldwide that causes a marked decrease in milk production in dairy cows. In the Northern hemisphere, where the disease is classically recognized, bovine coronavirus (BCoV) has been assigned as a major etiologic agent of the disease. Nonetheless, in the Southern hemisphere, an in-deep etiological survey on WD cases had not been carried out. This s...
Genetic characterization of Brazilian bovine viral diarrhea virus isolates by p...
Cortez,Adriana; Heinemann,Marcos B; Castro,Alessandra Marnie M.G. de; Soares,Rodrigo M; Pinto,Ana Maria V.; Alfieri,Amauri A.; Flores,Eduardo F.Nineteen isolates of bovine viral diarrhea virus (BVDV) from Brazil were genetically characterized through partial nucleotide sequencing and analysis of the 5'UTR region. The isolates were grouped as BVDV-1 (11/19), BVDV-2 (6/19) or "atypical" pestivirus (2/19). Among the BVDV-1, eight isolates were classified as subgenotype BVDV-1a, whereas most (4 out of 6) BVDV-2 belonged to subgenotype 2b. Two isolates from...