3 documents found, page 1 of 1
Effect of a combination of medium chain triglycerides, linoleic acid, soy lecit...
Magalhães, Maria Sonia Felício; Fechine, Francisco Vagnaldo; Macedo, Rafael Nogueira de; Monteiro, Diego Levi Silveira; Oliveira, Cecília CarvalhoPurpose: The aim of the study was to determine the effect of a combination of medium chain triglycerides (caprylic, capric, caproic and lauric acids), linoleic acid (essential fatty acid), vitamins A and E and soy lecithin, through a morphometric study, on the wound healing kinetics of experimental cutaneous ulcers. Methods: A total of 45 male Wistar rats were used, in which a skin flap of total thickness with ...
Alternative method for quantification of alfa-amylase activity
Farias, Davi Felipe; Hissa, Denise Cavalcante; Rocha-Bezerra, Lady Clarissa Brito da; Lima, Glauber Pacelli Gomes de; Carvalho, Ana Fontenele UranoA modification of the sensitive agar diffusion method was developed for macro-scale determination of alfa-amylase. The proposed modifications lower costs with the utilisation of starch as substrate and agar as supporting medium. Thus, a standard curve was built using alfa-amylase solution from Aspergillus oryzae, with concentrations ranging from 2.4 to 7,500 U.mL–1. Clear radial diffusion zones were measured af...
Purification of a PHA-like chitin-binding protein from Acacia farnesiana seeds ...
Santi-Gadelha, Tatiane; Rocha, Bruno Anderson Matias; Oliveira, Cecília Carvalho; Aragão, Karoline Sabóia; Marinho, EEmmanuel SilvaA lectin-like protein from the seeds of Acacia farnesiana was isolated from the albumin fraction, characterized, and sequenced by tandem mass spectrometry. The albumin fraction was extracted with 0.5 M NaCl, and the lectin-like protein of A. farnesiana (AFAL) was purified by ion-exchange chromatography (Mono-Q) followed by chromatofocusing. AFAL agglutinated rabbit erythrocytes and did not agglutinate human ABO...