46 documents found, page 1 of 5
Microbiome and Microbiota Within Wineries: A Review
Aires, Cristina; Maioto, Rita; Inês, António; Dias, Albino Alves; Rodrigues, Paula; Egas, Conceição; Sampaio, AnaThe main goal of this work is to review the winery’s microbiota, from the grape to the winery’s microbial niches (fermentation tanks, surfaces, air), and their risks to wine and human health. The impact of climate change on the winery microbiome and related challenges are also discussed. Microbial diversity in wineries depends on several factors, such as the grape variety and its ripeness, temperature, relative...
Genome sequence of the marine alphaproteobacterium sp. EG35 isolated from the t...
Keller-Costa, Tina; Madureira, Selene; Fernandes, Ana S.; Kozma, Lydia; Gonçalves, Jorge Manuel Santos; Barroso, Cristina; Egas, ConceiçãoWe report the genome sequence of sp. strain EG35 isolated from the octocoral sampled off the coast of Portugal. We reveal the coding potential for the biosynthesis of polyhydroxyalkanoates - biodegradable polyesters that may serve bioplastics production, diverse homoserine lactone-like communication signals, and four putatively novel natural products.
Multi-marker DNA metabarcoding for precise species identification in ichthyopla...
Ferreira, André O.; Barroso, Cristina; Azevedo, Olga Mouteira; Duarte, Sofia; Egas, Conceição; Fontes, João T.; Ré, Pedro; Santos, A. Miguel P.Ichthyoplankton monitoring is crucial for stock assessments, offering insights into spawning grounds, stock size, seasons, recruitment, and changes in regional ichthyofauna. This study evaluates the efficiency of multi-marker DNA metabarcoding using mitochondrial cytochrome c oxidase subunit I (COI), 12S rRNA and 16S rRNA gene markers, in comparison to morphology-based methods for fish species identification in...
Multi-marker DNA metabarcoding for precise species identification in ichthyopla...
Ferreira, André O.; Azevedo, Olga M.; Barroso, Cristina; Duarte, Sofia Alexandra Ferreira; Egas, Conceição; Fontes, João T.; Ré, PedroIchthyoplankton monitoring is crucial for stock assessments, offering insights into spawning grounds, stock size, seasons, recruitment, and changes in regional ichthyofauna. This study evaluates the efficiency of multi-marker DNA metabarcoding using mitochondrial cytochrome c oxidase subunit I (COI), 12S rRNA and 16S rRNA gene markers, in comparison to morphology-based methods for fish species identification in...
Extracellular vesicles improve GABAergic transmission in Huntington's disease i...
Beatriz, Margarida; Rodrigues, Ricardo J.; Vilaça, Rita; Egas, Conceição; Pinheiro, Paulo S.; Daley, George Q.; Schlaeger, Thorsten M.; Raimundo, NunoBackground: Extracellular vesicles (EVs) carry bioactive molecules associated with various biological processes, including miRNAs. In both Huntington’s disease (HD) models and human samples, altered expression of miRNAs involved in synapse regulation was reported. Recently, the use of EV cargo to reverse phenotypic alterations in disease models with synaptopathy as the end result of the pathophysiological casca...
Transcriptome analysis reveals the high ribosomal inhibitory action of 1,4-naph...
Cardoso, Joana M. S.; Esteves, Ivânia; Egas, Conceição; Braga, Mara E. M.; Sousa, Herminio C. de; Abrantes, Isabel; Maleita, CarlaThe root-knot nematode (RKN) Meloidogyne luci presents a threat to the production of several important crops. This nematode species was added to the European Plant Protection Organization Alert list in 2017. The scarce availability of efficient nematicides to control RKN and the phasing out of nematicides from the market have intensified the search for alternatives, such as phytochemicals with bionematicidal pr...
The Gut Microbiome Responds Progressively to Fat and/or Sugar-Rich Diets and Is...
Pessoa, João; Belew, Getachew D.; Barroso, Cristina; Egas, Conceição; Jones, John G.Describing diet-related effects on the gut microbiome is essential for understanding its interactions with fat and/or sugar-rich diets to promote obesity-related metabolic diseases. Here, we sequenced the V3-V4 hypervariable region of the bacterial 16S rRNA gene to study the composition and dynamics of the gut microbiome of adult mice fed diets rich in fat and/or sugar, at 9 and 18 weeks of diet. Under high-fat...
Genotype-first approach to identify associations between CDH1 germline variants...
Garcia-Pelaez, José; Barbosa-Matos, Rita; Lobo, Silvana; Dias, Alexandre dos Santos; Garrido, Luzia; Castedo, Sérgio; Sousa, SóniaBackground Truncating pathogenic or likely pathogenic variants of CDH1 cause hereditary diffuse gastric cancer (HDGC), a tumour risk syndrome that predisposes carrier individuals to diffuse gastric and lobular breast cancer. Rare CDH1 missense variants are often classified as variants of unknown significance. We conducted a genotype– phenotype analysis in families carrying rare CDH1 variants, comparing cancer s...
A primer-independent DNA polymerase-based method for competent whole-genome amp...
Ordóñez, Carlos D.; Mayoral-Campos, Carmen; Egas, Conceição; Redrejo-Rodríguez, ModestoMultiple displacement amplification (MDA) has proven to be a useful technique for obtaining large amounts of DNA from tiny samples in genomics and metagenomics. However, MDA has limitations, such as amplification artifacts and biases that can interfere with subsequent quantitative analysis. To overcome these challenges, alternative methods and engineered DNA polymerase variants have been developed. Here, we pre...
Understanding the Role of PIN Auxin Carrier Genes under Biotic and Abiotic Stre...
Cardoso, Hélia; Campos, Catarina; Grzebelus, Dariusz; Egas, Conceição; Peixe, AugustoThe PIN-FORMED (PIN) proteins represent the most important polar auxin transporters in plants. Here, we characterized the PIN gene family in two olive genotypes, the Olea europaea subsp. europaea var. sylvestris and the var. europaea (cv. ‘Farga’). Twelve and 17 PIN genes were identified for vars. sylvestris and europaea, respectively, being distributed across 6 subfamilies. Genes encoding canonical OePINs cons...