25 documents found, page 1 of 3
Direct lipid and carotenoid extraction from Rhodosporidium toruloides broth cul...
Dias, Carla; Nobre, B. P.; Santos, J. A. L.; Silva, Teresa Lopes da; Reis, AlbertoABSTRACT: Biodiesel produced from oleaginous microorganisms is considered an appealing option since oleaginous yeasts and microalgae can accumulate between 20 % and 80 % lipids of their dry cell weight. However, it is still not economically sustainable since production costs are still high. This work developed a new mild protocol using hexane or sunflower oil as extraction solvents to simultaneously extract lip...
Lipid and carotenoid production by a Rhodosporidium toruloides and Tetradesmus ...
Dias, Carla; Nobre, B. P.; Santos, J. A. L.; Reis, Alberto; Silva, Teresa Lopes daABSTRACT: In this study, Rhodosporidium toruloides and Tetradesmus obliquus were used for lipid and carotenoid production in mixed cultures using primary brewery wastewater (PBWW) as a culture medium, supplemented with sugarcane molasses (SCM) as a carbon source and urea as a nitrogen source. To improve biomass, lipid, and carotenoid production by R. toruloides and T obliquus mixed cultures, initial SCM concent...
Extraction of value-added compounds from microalgae
Ventura, S. P. M.; Nobre, B. P.; Ertekin, F.; Hayes, M.; Garciá-Vaquero, M.; Vieira, F.; Koc, M.; Gouveia, L.; Aires-Barros, M. R.; Palavra, A. M. F.Microalgae have been proposed as a multiproduct biorefinery feed stock. The algae-based biorefinery concept relies on the complete optimization process from the biomass production to processing and generating different products. Microalgae are composed of a high number of biocompounds with numerous applications. It has been pointed out by several researchers that one of the major process constraints in microalg...
The production of pigments & hydrogen through a Spirogyra sp. biorefinery
Pacheco, R.; Ferreira, Ana F.; Pinto, T.; Nobre, B. P.; Loureiro, David; Moura, Patrícia; Gouveia, Luisa; Silva, Carla M.This paper discusses the overall energy consumption and greenhouse gas emissions when extracting pigments and producing hydrogen from Spirogyra sp. microalga biomass. The energy evaluation from the biomass leftovers was also included in this work. The influence of the functional unit and different allocation criteria on the biorefinery assessments is also shown. The study consists of laboratory tests showing Sp...
The production of pigments & hydrogen through a Spirogyra sp. biorefinery
Pacheco, R.; Ferreira, Ana F.; Pinto, T.; Nobre, B. P.; Loureiro, David; Moura, Patrícia; Gouveia, Luisa; Silva, Carla M.This paper discusses the overall energy consumption and greenhouse gas emissions when extracting pigments and producing hydrogen from Spirogyra sp. microalga biomass. The energy evaluation from the biomass leftovers was also included in this work. The influence of the functional unit and different allocation criteria on the biorefinery assessments is also shown. The study consists of laboratory tests showing Sp...
Food and fuel microalgae applications: insights from portuguese experience [Res...
Batista, Ana Paula; Nobre, B. P.; Oliveira, Ana Cristina; Passarinho, Paula; Marques, Paula; Ribeiro, Belina; Raymundo, Anabela; Sousa, IsabelMicroalgae have a wide range of application fields, from food to fuels, to pharmaceuticals & fine chemicals, aquaculture and environmental bioremediation, among others. Spirulina and Chlorella have been used as food sources since ancient times, due to their high and balanced nutritional value. Our research group in Lisbon has developed a range of food products (emulsions, gelled desserts, biscuits and pastas) e...
Dual-mode cultivation of Chlorella protothecoides applying inter-reactors gas t...
Santos, Carla A.; Nobre, B. P.; Silva, Teresa Lopes da; Pinheiro, H. M.; Reis, AlbertoChlorella protothecoides, a lipid-producing microalga, was grown heterotrophically and autotrophicallyin separate reactors, the off-gases exiting the former being used to aerate the latter. Autotrophic biomass productivity with the two-reactor association, 0.0249 g L-1h-1, was 2.2-fold thevalue obtained in a control autotrophic culture, aerated with ambient air. Fatty acid productivity was1.7-fold the control v...
Effect of light on the production of bioelectricity and added-value microalgae ...
Gouveia, Luisa; Neves, Carole; Sebastião, Diogo; Nobre, B. P.; Matos, Cristina T.This study demonstrates the simultaneous production of bioelectricity and added-value pigments in a Photosynthetic Alga Microbial Fuel Cell (PAMFC). A PAMFC was operated using Chlorella vulgaris in the cathode compartment and a bacterial consortium in the anode. The system was studied at two different light intensities and the maximum power produced was 62.7 mW/m2 with a light intensity of 96 lE/(m2 s). The res...
Dual-mode cultivation of Chlorella protothecoides applying inter-reactors gas t...
Santos, Carla A.; Nobre, B. P.; Silva, Teresa Lopes da; Pinheiro, H. M.; Reis, AlbertoChlorella protothecoides, a lipid-producing microalga, was grown heterotrophically and autotrophicallyin separate reactors, the off-gases exiting the former being used to aerate the latter. Autotrophic biomass productivity with the two-reactor association, 0.0249 g L-1h-1, was 2.2-fold thevalue obtained in a control autotrophic culture, aerated with ambient air. Fatty acid productivity was1.7-fold the control v...
New at-line flow cytometric protocols for determining carotenoidcontent and cel...
Freitas, Claúdia; Nobre, B. P.; Gouveia, Luisa; Roseiro, J. Carlos; Reis, Alberto; Silva, Teresa Lopes daRhodosporidium toruloides NCYC 921 batch growth was monitored as a means to evaluate the yeastbiomass potential as a source for the production of carotenoids and other lipids.Carotenoid content, cell viability and size were assessed by multiparameter flow cytometry. Thesaponifiable lipid fraction was assayed by gas–liquid chromatography.The carotenoid production increased during the stationary phase, reaching 7...