104 documents found, page 1 of 11
Combination of phages and antibiotics with enhanced killing efficacy against du...
Akturk, Ergun; Pinto, Graça; Ostyn, Lisa; Crabbé, Aurélie; Melo, Luís Daniel Rodrigues; Azeredo, Joana; Coenye, TomPseudomonas aeruginosa and Staphylococcus aureus are opportunistic pathogens commonly found in biofilm-associated polymicrobial respiratory infections that are challenging to control. Studies performed in laboratory standard conditions suggest that bacterio(phages) and antibiotic combinations are more active against bacterial communities and biofilms than each agent alone. The purpose of this work was to study ...
Screening and in silico characterization of prophages in Helicobacter pylori cl...
Ferreira, Rute Vanessa Novais; Pinto, Graça; Presa, Eva; Oleastro, Mónica; Silva, Catarina; Vieira, Luís; Sousa, Cláudia Sofia CunhaThe increase of antibiotic resistance calls for alternatives to control Helicobacter pylori, a Gram-negative bacterium associated with various gastric diseases. Bacteriophages (phages) can be highly effective in the treatment of pathogenic bacteria. Here, we developed a method to identify prophages in H. pylori genomes aiming at their future use in therapy. A polymerase chain reaction (PCR)-based technique test...
Successful bacteriophage therapy of pyoderma caused by methicillin-resistant St...
Mota-Sá, Cátia; Rodrigues, Cláudia; Silva, Vanessa; Azeredo, Joana; Melo, Luís Daniel RodriguesPyoderma caused by methicillin-resistant Staphylococcus pseudintermedius poses significant challenges to canine health. Successful therapy requires an integrated approach to prudent antimicrobial use. This report describes the successful treatment of a dog with generalised superficial pyoderma using a topical formulation of S. pseudintermedius phage applied once daily for a period of 30 days.; A piodermite caus...
Exploring in vitro efficacy of rCHAPk with antibiotic combinations, and promisi...
Tasdurmazli, Semra; Cinar, Irfan; Karamese, Murat; Aksak Karamese, Selina; Cadirci, Elif; Melo, Luís Daniel Rodrigues; Ozbek, TulinThe increasing threat of antimicrobial-resistant bacteria, particularly Staphylococcus aureus, which rapidly develops multidrug resistance and commonly colonizes wound surfaces, demands innovative strategies. Phage-encoded endolysins offer a dual-purpose approach as topical therapies for infectious skin wounds and synergistic agents to reduce high-dose antibiotic dependence. This study explores recombinant CHAP...
CRISPR‑Cas9 engineered Saccharomyces cerevisiae for endolysin delivery to comba...
Sáez Moreno, David; Cunha, Joana; Melo, Luís Daniel Rodrigues; Tanaka, Kenya; Bamba, Takahiro; Hasunuma, Tomosiha; Azeredo, Joana; Domingues, LucíliaListeriosis is an infection caused by the consumption of food contaminated with Listeria monocytogenes. It leads to febrile gastroenteritis, central nervous system infections, and even death in risk populations. Bacteriophage endolysins selectively kill bacteria hydrolyzing their cell walls and have emerged as a potential tool for listeriosis control. Ply511 is an anti-Listeria endolysin that has activity again...
Coagulase-Negative Staphylococci phages panorama: genomic diversity and in vitr...
Lopes, Maria Beatriz Afonso Sequeira; Silva, Maria Daniela Ferreira; Azeredo, Joana; Melo, Luís Daniel RodriguesCoagulase-negative staphylococci (CoNS) are commensal bacteria of the human skin and mucosal membranes. The incidence of nosocomial infections caused by these species is on the rise, leading to a potential increase in antibiotic tolerance and resistance. Phages are emerging as a promising alternative to combat CoNS infections. Scientists are isolating phages infecting CoNS with a particular interest in S. epide...
PLGA nanoparticles double-decorated with a TAT peptide and folic acid to target...
Andrade, Stéphanie; Ramalho, Maria J.; Santos, João; Santos, Sílvio Roberto Branco; Melo, Luís Daniel Rodrigues; Guimarães, Nuno; Ferraz, Maria P.Treating bacterial infections has become increasingly difficult due to the rise in antibiotic-resistant bacterial strains. Strategies involving the targeted delivery of antibiotics have been proposed to minimize the administered antibiotic doses. This study aims to develop the first double-modified nanovehicle capable of increasing bacterial membranes permeability while specifically targeting Staphylococcus aur...
Novas perspetivas sobre o papel e as aplicações dos bacteriófagos de Helicobact...
Ferreira, Rute Vanessa Novais; Oleastro, Mónica; Azeredo, Joana; Figueiredo, Céu; Melo, Luís Daniel RodriguesAs infeções por Helicobacter pylori são caracterizadas por uma elevada incidência global, constituindo uma ameaça significativa à saúde pública. Os tratamentos atuais envolvem a administração de antibióticos e inibidores da bomba de protões. No entanto, esta abordagem terapêutica apresenta uma eficácia limitada, principalmente devido ao aumento da resistência aos antibióticos em todo o mundo. Este cenário salie...
Comparative RNA-Seq analysis of S. epidermidis underSEP1 phage infection during...
Silva, Maria Daniela; Pinto, Graça; França, Angela; Azeredo, Joana; Melo, Luís Daniel RodriguesBacteria in nature often exist in the stationary phase, where antimicrobials, including phages, are less effective. The SEP1 phage was shown to reduce stationary S. epidermidis cells. This study analysed the response of both exponential and stationary cells to SEP1 infection. For that, RNA was extracted at 0, 5, 15, and 30 min post-infection and analysed by RNA-seq. In exponential cells, only 3 genes were upreg...
Phage-host interaction with cells in different metabolic states: A S. epidermid...
Melo, Luís Daniel Rodrigues; Silva, Maria Daniela Ferreira; Pinto, Graça; França, Ângela Maria Oliveira Sousa; Azeredo, JoanaIn nature, bacteria are not frequently found in the exponential state of growth. One particular issue is that the efficacy of antimicrobials, including phages, is always tested against bacterial cells at their highest growth rate. The majority of bacterial biomass occur in the form of a biofilm. Biofilms have a high tolerance to antimicrobial agents, mainly, due to the low metabolic activity of the biofilm cell...