13 documents found, page 1 of 2
Design of liposomes as drug delivery system for therapeutic applications
Guimarães, Diana Isabel Pereira; Cavaco-Paulo, Artur; Nogueira, E.Liposomes are spherical vesicles consisting of one or more concentric phospholipid bilayers enclosing an aqueous core. Being both nontoxic and biodegradable, liposomes represent a powerful delivery system for several drugs. They have improved the therapeutic efficacy of drugs through stabilizing compounds, overcoming obstacles to cellular and tissue uptake and increasing drug biodistribution to target sites in ...
Protective effect of saccharides on freeze-dried liposomes encapsulating drugs
Guimarães, Diana Isabel Pereira; Noro, Jennifer Martins; Silva, Carla; Cavaco-Paulo, Artur; Nogueira, E.The production of freeze-dried liposomes encapsulating drugs is considered a key challenge since the drugs are prone to leakage. The aim of this work was to study the effect of different saccharides on preserving the stability and drug retention capacity of a previously developed liposomal formulation, when subjected to a freeze-drying process. The protective role of trehalose, lactose, glucose, mannitol and su...
OBP fused with cell-penetrating peptides promotes liposomal transduction
Gonçalves, Filipa D.; Castro, T.; Nogueira, E.; Pires, Ricardo; Silva, Carla Manuela Pereira Marinho; Ribeiro, Artur; Cavaco-Paulo, ArturCell-penetrating peptides (CPPs) have been applied as novel transport systems with the ability to facilitate the delivery of peptides, proteins, and oligonucleotides into cells. Herein, we designed different fusion proteins composed by pig odorant binding protein (OBP-I) and three CPPs, namely Tat, pVEC and Pep-1. A new methodology using liposomes as reservoirs and OBP:CPPs as carriers was developed as an advan...
Internalization of methotrexate conjugates by folate receptor-α
Nogueira, E.; Passos, M.; Azóia, Nuno G.; Antunes, Egipto; Loureiro, Ana; Guimarães, Diana Isabel Pereira; Jennifer Noro; Rollett, AlexandraThe folate antagonist methotrexate is a cytotoxic drug used in the treatment of several cancer types. Methotrexate entry into the cell is mediated by two main transport systems: the reduced folate carrier and membrane-associated folate receptors. These transporters differ considerably in their mechanism of (anti)folate uptake, substrate specificity and tissue specificity. Although the mechanism of action of the...
Fab antibody fragment-functionalized liposomes for specific targeting of antige...
Ohradanova-Repic, Anna; Nogueira, E.; Hartl, Ingrid; Gomes, Andreia C; Preto, Ana; Steinhuber, Eva; Mühlgrabner, Vanessa; Repic, Marko; Kuttke, MarioLiposomes functionalized with monoclonal antibodies or their antigen-binding fragments have attracted much attention as specific drug delivery devices for treatment of various diseases including cancer. The conjugation of antibodies to liposomes is usually achieved by covalent coupling using cross-linkers in a reaction that might adversely affect the characteristics of the final product. Here we present an alte...
The absence of albumin improves in vitro cellular uptake and disruption of Polo...
Loureiro, Ana Isabel Sá; Jennifer Noro; Abreu, Ana S.; Nogueira, E.; Soares da Costa, Diana; Silva, Carla; Cavaco-Paulo, ArturNovel nanoparticles based on Poloxamer 407 and vegetable oil were produced by high pressure homogenization. Functionalization of those nanoparticles was made by incorporation of folic acid (FA)-Poloxamer 407 conjugate. These nanoparticles showed suitable characteristics for intravenous therapeutic applications similarly to PEGylated albumin-based nanoparticles, previously described by our research group. Here, ...
Comportamento alimentar de vacas Nelore e de seus bezerros puros ou mestiços
Rodrigues, W. B. [UNESP]; Wechsler, F. S. [UNESP]; Costa, D. S.; Nogueira, E.Made available in DSpace on 2018-12-11T16:43:04Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-01-01. Added 1 bitstream(s) on 2021-07-15T14:32:41Z : No. of bitstreams: 1 S0102-09352016000300596.pdf: 203758 bytes, checksum: 65b2047cffcc3e8c7722c1fc929a6944 (MD5); The aim of this study was to evaluate the effect of a calf's genetic group on the behavior of dam and calf. We used 24 Nellore dams and their c...
Liposomal formulations for rheumatoid arthritis
Nogueira, E.; Cavaco-Paulo, Artur[Excerpt] Rheumatoid arthritis (RA) is the most common inflammatory rheumatic disease, affecting almost 1% of the world population. Although the cause of RA remains unknown, the complex interaction between immune mediators (cytokines and effector cells) is responsible for the joint damage that begins at the synovial membrane. Activated macrophages are critical in the pathogenesis of RA and showed specifically e...
Neutral PEGylated liposomal formulation for efficient folate-mediated delivery ...
Nogueira, E.; Freitas, Jaime; Loureiro, Ana Isabel Sá; Nogueira, Patrícia; Gomes, Andreia C; Preto, Ana; Carmo, Alexandre M.; Moreira, AlexandraCationic liposomes are efficient vectors for systemic delivery of therapeutic small interfering RNA (siRNA), taking advantage of RNA interference (RNAi), a naturally occurring gene-silencing mechanism in mammalian cells. However, toxicity at high concentrations, short circulating half-lives and lack of specificity restrict their successful application in a wider scale. The purpose of this study was to evaluate ...
Assessment of liposome disruption to quantify drug delivery in vitro
Nogueira, E.; Cruz, Célia F.; Loureiro, A.; Nogueira, Patrícia; Freitas, Jaime; Moreira, Alexandra; Carmo, Alexandre M.; Gomes, Andreia C; Preto, AnaEfficient liposome disruption inside the cells is a key for success with any type of drug delivery system. The efficacy of drug delivery is currently evaluated by direct visualization of labeled liposomes internalized by cells, not addressing objectively the release and distribution of the drug. Here, we propose a novel method to easily assess liposome disruption and drug release into the cytoplasm. We propose ...