2 documents found, page 1 of 1
First molecular detection of Eimeria spp. in eared doves (Zenaida auriculata) f...
Taroda, Alessandra; Barros, Luiz Daniel de; Seixas, Mércia de; Cardim, Sérgio Tosi; Sasse, João Pedro; Minutti, Ana Flávia; Vidotto, OdilonThe aim of the present study was to detect Eimeria spp. in eared doves (Zenaida auriculata) from Brazil. Two hundred and fourteen birds were trap-capture in different regions of Londrina city, Paraná state, Southern Brazil. Fecal samples were collected, and DNA extraction was performed. A nested PCR based on the subunit I of the cytochrome c oxidase gene of the Eimeria mitochondrial genome was used to detect th...
Development of a qPCR to diagnose the genus Eimeria in bovines
Cardim, Sérgio Tosi; Cardim, Stefani Lino; Seixas, Mércia de; Taroda, Alessandra; Carneiro, Priscilla Gomes; Zulpo, Dauton Luiz; Barros, Luiz Daniel deBovine coccidiosis is caused by protozoa of the genus Eimeria. These protozoa mainly affect young animals, causing a decrease in production and consequent economic losses. The routine diagnosis is made through morphological observation of the oocysts, which has several limitations. The objective of the present study was to develop a qPCR technique for the diagnose of Eimeria spp. in cattle. For this purpose, th...