50 documents found, page 1 of 5
Ageing affects the CD4+ T cell polarization and mucosal tropism induced by TLR2...
Zúquete, Sara; Ferreira, Mariana; Delgado, Inês L.; Rosa, Maria teresa; Mendes, Ana Catarina; Santos, Dulce; Nolasco, Sofia; Graça, LuísToll-like receptor (TLR)2 activation induces aldehyde dehydrogenase enzymes in non-mucosal dendritic cells (DCs), enabling them to metabolize vitamin A into all-trans retinoic acid, which induces the expression of mucosal-homing molecules (α4β7 and CCR9) in the activated T cells. Recently, we have shown that the simultaneous activation of non-mucosal DCs through TLR2 and TLR4 maintains such capacity while reinf...
Tubulin acetylation and the cellular mechanosensing and stress response
Carmona, Bruno; Delgado, Inês L. S.; Nolasco, Sofia; Marques, Rita; Gonçalves, João; Soares, HelenaMicrotubule (MT) acetylation has emerged as a critical regulator of cellular stress responses, integrating mechanical and oxidative stimuli to support cellular adaptability and survival. This post-translational modification (PTM) enhances MT flexibility and resilience, enabling cells to withstand mechanical challenges such as changes in extracellular matrix stiffness and applied forces. Through its impact on MT...
Tubulin acetylation: a critical regulator of microtubule function
Delgado, Inês L.; Carmona, Bruno; Nolasco, Sofia; Marques, Rita; Gonçalves, João; Soares, HelenaThe cytoskeleton is conserved throughout the eukaryotic lineage and consists of a complex dynamic network mainly composed of three distinct polymers: microtubules (MTs), actin filaments, and intermediate filaments. MTs are polymers of α/β-tubulin heterodimers, playing a myriad of distinct cellular functions, and are the main components of complex structures like the mitotic spindle, cilia, and centrioles. Post-...
Balancing act: tubulin glutamylation and microtubule dynamics in Toxoplasma gondii
Delgado, Inês; Gonçalves, João; Fernandes, Rita; Zúquete, Sara; Basto, Afonso P.; Leitão, Alexandre; Soares, Helena; Nolasco, SofiaThe success of the intracellular parasite Toxoplasma gondii in invading host cells relies on the apical complex, a specialized microtubule cytoskeleton structure associated with secretory organelles. The T. gondii genome encodes three isoforms of both α- and β-tubulin, which undergo specific post-translational modifications (PTMs), altering the biochemical and biophysical proprieties of microtubules and modulat...
Balancing act: tubulin glutamylation and microtubule dynamics in Toxoplasma gondii
Delgado, Inês L.S.; Gonçalves, João; Fernandes, Rita; Zúquete, Sara; Basto, Afonso P.; Leitão, Alexandre; Soares, Helena; Nolasco, SofiaThe success of Toxoplasma gondii (intracellular parasite) host cell invasion relies on the apical complex, a specialized microtubule cytoskeleton structure associated with secretory organelles. The genome encodes three isoforms of both α- and β-tubulin which are altered by specific post-translational modifications (PTMs), changing the biochemical/biophysical proprieties of microtubules, and modulating their int...
Combined TLR2/TLR4 activation equip non-mucosal dendritic cells to prime Th1 ce...
Zúquete, Sara; Ferreira, Mariana; Delgado, Inês L. S.; Gazalle, Paula; Andaluz, Stephanie; Rosa, Maria Teresa; Mendes, Ana Catarina; Santos, DulceActivated CD4+ T cells located at mucosal surfaces orchestrate local effector immune mechanisms. When properly polarized, these cells contribute to block infections at early stages and may be essential to restrain the local growth of mucosal tumors, playing a critical role in host protection. How CD4+ T cells simultaneously integrate gut-homing instructions and Th polarization signals transmitted by TLR-activat...
Biological Effects induced by Magnetic Resonance Imaging
Pedra, Carla; Nolasco, Sofia; Belchior, AnaIntroduction: Magnetic Resonance Imaging (MRI) represents one of the greatest technological advances in terms of medical imaging technique used in radiology. Due to the increased number of pathologies diagnosed by MRI, the number of patients has been increasing over the years, and consequently there is an increase of patients’ exposure to non-ionizing radiation. This exposure reveals to be a concern due to the ...
Tick-borne diseases in asymptomatic cattle from São Miguel Island, Azores – A c...
Felício, Maria; Zúquete, Sara; Lima, Patrícia; Delgado, Inês; Reis, Pedro; Romão, Ricardo; Nolasco, Sofia; Santos, Dulce; Basto, Afonso; Padre, LudovinaTick-borne diseases in asymptomatic cattle from São Miguel Island, Azores – A clinical perspective Introduction/Background: The Azores archipelago is one of the regions with the highest cattle production, in Portugal. Outbreaks of tick-borne (TB) diseases have been reported by local field veterinarians and different tick species are described in São Miguel. At this study, we decided to investigate the presence ...
The nuclear levels of thioredoxin reductase 1, gamma-H2AX, and yap are modulate...
Marques, Rita; Ginete, Catarina; Veiga, Luisa; Brito, Miguel; Soares, Helena; Carmona, Bruno; Paiva, Mariana; Nolasco, Sofia; Marinho, Susana H.Diabetes is a condition characterized by impaired regulation of blood glucose levels, leading to various complications such as hypertension, cardiovascular disease, and retinopathy. Diabetic retinopathy (DR), caused by a disrupted retinal blood barrier, is associated with oxidative stress resulting from dysregulated glucose levels in the retina. The primary cilium, an organelle involved in energy balance and gl...
Os cílios primários regulam os níveis de tiorredoxina redutase 1 e de yH2AX em ...
Marques, Rira; Paiva, Mariana; Ginete, Catarina; Nolasco, Sofia; Marinho, Susana H.; Veiga, Luisa; Brito, Miguel; Soares, Helena; Carmona, BrunoA diabetes caracteriza-se por uma anormal capacidade de controlar o nível de glucose na corrente sanguínea, podendo levar a outras complicações, tais como hipertensão, doenças cardiovasculares, e retinopatia. A desregulação dos níveis de glucose na retina tem demonstrado aumentar os níveis de peróxido de hidrogénio, conduzindo a uma rutura na barreira sanguínea da retina, uma das causas de retinopatia diabética...