9 documents found, page 1 of 1
Saccharomyces cerevisiae cells lacking the zinc vacuolar transporter Zrt3 displ...
Terra-Matos, Joana; Teixeira, Marta Oliveira; Pereira, Cátia Sofia Santos; Noronha, Henrique; Domingues, Lucília; Sieiro, Carmen; Gerós, H.Yeast-based bioethanol production from lignocellulosic hydrolysates (LH) is an attractive and sustainable alternative for biofuel production. However, the presence of acetic acid (AA) in LH is still a major problem. Indeed, above certain concentrations, AA inhibits yeast fermentation and triggers a regulated cell death (RCD) process mediated by the mitochondria and vacuole. Understanding the mechanisms involved...
VviRafS5 is a raffinose synthase involved in cold acclimation in grapevine wood...
Noronha, Henrique; Silva, Angélica; Silva, Tiago; Frusciante, Sarah; Diretto, Gianfranco; Gerós, H.The accumulation of raffinose family oligosaccharides (RFOs) is a hallmark of plant response to different abiotic stresses, including cold. The synthesis of galactinol, by galactinol synthases (GolS), and raffinose, by raffinose synthases (RafS), are fundamental for stress-induced accumulation of RFOs, but the role of these enzymes in the cold response of grapevine (Vitis vinifera L.) woody tissues is still unc...
Grapevine aquaporins: Diversity, cellular functions, and ecophysiological persp...
Sabir, Farzana; Zarrouk, Olfa; Noronha, Henrique; Loureiro-Dias, M. C.; Soveral, Graça; Gerós, Hernâni; Prista, CatarinaHigh-scored premium wines are typically produced under moderate drought stress, suggesting that the water status of grapevine is crucial for wine quality. Aquaporins greatly influence the plant water status by facilitating water diffusion across the plasma membrane in a tightly regulated manner. They adjust the hydraulic conductance of the plasma membrane rapidly and reversibly, which is essential in specific p...
Sweet Cherry (Prunus avium L.) PaPIP1;4 is a functional aquaporin upregulated b...
Breia, Richard Maykel Gonçalves; Mósca, Andreia F.; Conde, Artur; Correia, Sofia; Conde, Carlos; Noronha, Henrique; Soveral, Graça; Gonçalves, BertaThe involvement of aquaporins in rain-induced sweet cherry (Prunus avium L.) fruit cracking is an important research topic with potential agricultural applications. In the present study, we performed the functional characterization of PaPIP1;4, the most expressed aquaporin in sweet cherry fruit. Field experiments focused on the pre-harvest exogenous application to sweet cherry trees, cultivar Skeena, with a sol...
Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is as...
Teixeira, António; Martins, Viviana; Frusciante, Sarah; Cruz, Telmo; Noronha, Henrique; Diretto, Gianfranco; Gerós, H.Flavescence dorée (FD), caused by the phytoplasma Candidatus Phytoplasma vitis, is a major threat to vineyard survival in different European grape-growing areas. It has been recorded in French vineyards since the mid-1950s, and rapidly spread to other countries. In Portugal, the phytoplasma was first detected in the DOC region of Vinhos Verdes in 2006, and reached the central region of the country in 2009. The ...
The grapevine (Vitis vinifera) aquaporin VvNIP2;1 is a silicon channel localize...
Noronha, Henrique; Silva, Angélica; Mitani-Ueno, Namiki; Conde, Carlos; Sabir, Farzana; Prista, Catarina; Soveral, Graça; Isenring, Paul; Feng Ma, JiangSilicon (Si) supplementation has been shown to improve plant tolerance to different stresses and its accumulation in the aerial organs is mediated by NIP2;1 aquaporins (Lsi channels) and Lsi2-type exporters in roots. In the present study, we tested the hypothesis that grapevine expresses a functional NIP2;1 that accounts for root Si uptake and, eventually, Si accumulation in leaves. Own-rooted grapevine cutting...
A molecular perspective on starch metabolism in woody tissues
Noronha, Henrique; Silva, Angélica; Dai, Zhanwu; Gallusci, Philippe; Rombolà, Adamo D.; Delrot, Serge; Gerós, H.The elucidation of the molecular mechanisms of starch synthesis and mobilization in perennial woody tissues is of the utmost scientific and agricultural importance. Starch is the main carbohydrate reserve in plants and is fundamental in human nutrition and several industrial processes. In leaves, starch accumulated during the day is degraded throughout the night and the resulting sugars, glucose and maltose, ar...
Low source-sink ratio reduces reserve starch in grapevine woody canes and modul...
Silva, Angélica; Noronha, Henrique; Dai, Zhanwu; Delrot, Serge; Gerós, H.Severe leaf removal decreases storage starch and sucrose in grapevine cv. Cabernet Sauvignon fruiting cuttings and modulates the activity of key enzymes and the expression of sugar transporter genes.
The grapevine uncharacterized intrinsic protein 1 (VvXIP1) is regulated by drou...
Noronha, Henrique; Araujo, Diogo; Conde, Carlos; Martins, Ana P.; Soveral, Graca; Chaumont, Francois; Delrot, Serge; Gerós, H.A MIP (Major Intrinsic Protein) subfamily called Uncharacterized Intrinsic Proteins (XIP) was recently described in several fungi and eudicot plants. In this work, we cloned a XIP from grapevine, VvXIP1, and agrobacterium-mediated transformation studies in Nicotiana benthamiana revealed that the encoded aquaporin shows a preferential localization at the endoplasmic reticulum membrane. Stopped-flow spectrometry ...