13 documents found, page 1 of 2
Epidemiology and discrimination of clinically relevant Enterobacter cloacae com...
Martins, Rita; Gonçalves, Ana Beatriz; Marques Silva, Luís; Castro, Ana Paula; Read, Maria Antónia; Alves, Valquíria; Peixe, Luísa; Novais, ÂngelaBackground: E. cloacae complex species are increasingly implicated in infections caused by multidrug-resistant bacteria, but their epidemiology is scarce due to the limitations of automated methods in accurate species identification (e.g. VITEK2/ MALDI–TOF MS) [1]. FT-IR is a promising quick, simple and low-cost alternative for bacterial discrimination [2]. Objective: We aim to assess the epidemiology of Entero...
Decoding Klebsiella pneumoniae in poultry chain: Unveiling genetic landscape, a...
Mourão, Joana; Magalhães, Mafalda; Ribeiro-Almeida, Marisa; Rebelo, Andreia; Novais, Carla; Peixe, Luísa; Novais, Ângela; Antunes, PatríciaThe rise of antibiotic resistance in the food chain is influenced by the use of antimicrobial agents, such as antibiotics, metals, and biocides, throughout the entire farm-to-fork continuum. Besides, non-clinical reservoirs potentially contribute to the transmission of critical pathogens such as multidrug-resistant (MDR) Klebsiella pneumoniae. However, limited knowledge exists about the population structure and...
From Farm to Fork: Persistence of Clinically Relevant Multidrug-Resistant and C...
Mourão, Joana; Ribeiro-Almeida, Marisa; Novais, Carla; Magalhães, Mafalda; Rebelo, Andreia; Ribeiro, Sofia; Peixe, Luísa; Novais, ÂngelaConcerns about colistin-resistant bacteria in animal food-environmental-human ecosystems prompted the poultry sector to implement colistin restrictions and explore alternative trace metals/copper feed supplementation. The impact of these strategies on the selection and persistence of colistin-resistant Klebsiella pneumoniae in the whole poultry production chain needs clarification. We assessed colistin-resistan...
From farm-to-fork: occurrence of persistent multidrug-resistant and copper-tole...
Magalhães, Mafalda; Almeida, Marisa Ribeiro; Mourão, Joana; Rebelo, Andreia; Lopes, Diana; Peixe, Luísa; Novais, Ângela; Novais, CarlaKlebsiella pneumoniae-Kp is a clinically-relevant but neglected multidrugresistant-MDR bacteria at the One Health interface. Studies on poultry production to depict factors contributing to Kp selection/persistence remain scarce. Here we evaluated Kp occurrence and diversity among chicken flocks, its environment and derived meat produced in 7 intensive farms-FA where copper-supplemented feeds are widely used.
High diversity of pathogenic Escherichia coli clones carrying mcr‐1 among gulls...
Ribeiro‐Almeida, Marisa; Mourão, Joana; Novais, Ângela; Pereira, Sofia; Freitas‐Silva, Joana; Ribeiro, Sofia; Martins da Costa, Paulo; Peixe, LuísaThe expansion of mcr-carrying bacteria is a well-recognized public health problem. Measures to contain mcr spread have mainly been focused on the food-animal production sector. Nevertheless, the spread of MCR producers at the environmental interface particularly driven by the increasing population of gulls in coastal cities has been less explored. Occurrence of mcr-carrying Escherichia coli in gull's colonies f...
Fourier transform infrared (FT-IR) spectroscopy typing: a real-time analysis of...
Silva, Liliana; Rodrigues, Carla; Lira, Agostinho; Leão, Mariana; Mota, Margarida; Lopes, Paulo; Novais, Ângela; Peixe, LuísaThe spread of multidrug-resistant (MDR) Klebsiella pneumoniae in the nosocomial setting represents a big challenge to infection control teams. We have recently developed a simple spectroscopic-based method with excellent accuracy, turnaround time and cost-effectiveness (Rodrigues et al. mSystems 2020) for bacterial typing. Here, we applied our method in a real clinical context to support early identification of...
Commonality of multidrug-resistant klebsiella pneumoniae ST348 isolates in hors...
Trigo Da Roza, Filipa; Couto, Natacha; Carneiro, Carla; Cunha, Eva; Rosa, Teresa; Magalhães, Mariana; Tavares, Luis; Novais, Ângela; Peixe, LuísaABSTRACT - Multidrug-resistant (MDR) Klebsiella pneumoniae is considered a major global concern by the World Health Organization. Evidence is growing on the importance of circulation of MDR bacterial populations between animals and humans. Horses have been shown to carry commensal isolates of this bacterial species and can act as human MDR bacteria reservoirs. In this study, we characterized an extended-spectru...
Citrobacter portucalensis sp. nov., isolated from an aquatic sample
Ribeiro, Teresa Gonçalves; Gonçalves, Bruno; Silva, Mickael; Novais, Ângela; Machado, Elisabete; Carrico, Joao Andre; Peixe, LuísaA Gram-stain-negative strain, A60T, isolated from a water well sample in Portugal, was characterized phenotypically, genotypically and phylogenetically. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain A60T belonged to the genus Citrobacter, and recN gene phylogeny revealed one strongly supported clade encompassing strain A60T and 13 other strains from public databases, distinct from...
Long-term dissemination of acquired AmpC β-lactamases among Klebsiella spp. and...
Freitas, Francisco; Machado, Elisabete; Ribeiro, Teresa; Novais, Ângela; Peixe, LuísaWe investigated the occurrence, diversity and molecular epidemiology of genes coding for acquired AmpC β-lactamases (qAmpC) among clinical isolates of Enterobacteriaceae lacking inducible chromosomal AmpCs in Portugal. A total of 675 isolates non-susceptible to broad-spectrum cephalosporins obtained from four hospitals and three community laboratories during a 7-year period (2002-2008) were analysed. The presen...
Characterization of the first extended-spectrum beta-lactamase producing nontyp...
Antunes, Patrícia; Mourão, Joana; Alves, Tatiana; Campos, Joana; Novais, Carla; Novais, Ângela; Peixe, Luísa[abstract]