131 documents found, page 1 of 14

Identification of linezolid-resistant Enterococcus spp. in calves: findings fro...

Background: Antimicrobial resistance (AMR) is a global health threat requiring a One Health approach, as antibiotic-resistant bacteria can spread between animals and humans. Enterococcus spp., particularly E. faecium (Efm) and E. faecalis (Efs), are key AMR indicators due to their role as gut commensals and their potential as reservoirs of resistance genes. They are also opportunistic pathogens that can cause s...

Insights towards the impact of subinhibitory chlorhexidine on antimicrobial sus...

Enterococcus faecium, a human and animal commensal broadly distributed in the environment, is currently one of the most challenging multidrug-resistant (MDR) healthcare-associated pathogens worldwide. It is often exposed to chlorhexidine (CHX), a broad-spectrum antiseptic, extensively used in healthcare, domestic, and food production settings, and a diffused polluter. However, the impact of gradients of CHX con...

Colonization and diversity of Klebsiella pneumoniae species complex (KpSC) in t...

Decoding Klebsiella pneumoniae in poultry chain: Unveiling genetic landscape, a...

The rise of antibiotic resistance in the food chain is influenced by the use of antimicrobial agents, such as antibiotics, metals, and biocides, throughout the entire farm-to-fork continuum. Besides, non-clinical reservoirs potentially contribute to the transmission of critical pathogens such as multidrug-resistant (MDR) Klebsiella pneumoniae. However, limited knowledge exists about the population structure and...

Bacteriocin dynamics in Enterococcus faecium and Enterococcus lactis: implicati...

Background: Enterococcus faecium-Efm and E.lactis-Elts (former Efm-cladeB) colonize the human gut, with Efm also being a leading hospital-pathogen. Dynamics influencing strain dominance in competitive environments (e.g., infection/colonization) are not fully understood, but bacteriocins may provide competitive advantage to clinical Efm or commensal Elts strains. Objective: We explored bacteriocin content of con...

A challenge in antibiotic stewardship: detection of vancomycin-variable Enteroc...

Background: Vancomycin-variable-enterococci (VVE) are vanA+ enterococci expressing a vancomycin-susceptible phenotype that can revert to a resistant phenotype (VRE) after vancomycin exposure. Objective: We aimed to screen and characterize VVE in a large collection of Enterococcus faecium (Efm) [1]. Methods: We performed a vanA-PCR screening on an extensive Efm collection (2009-2022), including hospital (n=255) ...

Characterization of vancomycin-resistant Enterococcus faecium causing infection...

Background: Vancomycin-resistant Enterococcus faecium (VREfm) are leading nosocomial pathogens linked to high mortality rates and costs [1,2]. VREfm are also included in the WHO global priority list of antibiotic-resistant bacteria for which new antibiotics are urgently needed [3]. Their epidemiology is puzzling within Europe and VREfm data in Portuguese hospitals are lacking since the 2000s. Objective: We...

Avaliação da Higiene de Superfícies em Unidades de Restauração Coletiva e a Rel...

Raw meat-based diet for pets: a neglected source of human exposure to Salmonell...

Background: The pet industry is expanding worldwide, particularly raw meat -based diets (RMBDs). There are concerns regarding the safety of RMBDs, especially their potential to spread clinically relevant antibioticresistant bacteria or zoonotic pathogens. Aim: We aimed to investigate whether dog food, including RMBD, commercially available in Portugal can be a source of Salmonella and/or other Enterobacteriacea...

Pet food safety: emerging bacterial hazards and implications for public health

Bacterial hazards in pet food, especially in raw diets, are a current public health issue to both pets and humans. The most substantial body of evidence and consequent risk stems from food-borne pathogens such as Salmonella and bacteria resistant to last-resort antibiotics (e.g. colistin, third generation of cephalosporins, linezolid). State-of-the-art methods, particularly whole-genome sequencing, have been fu...