Background: Antimicrobial resistance (AMR) is a global health threat requiring a One Health approach, as antibiotic-resistant bacteria can spread between animals and humans. Enterococcus spp., particularly E. faecium (Efm) and E. faecalis (Efs), are key AMR indicators due to their role as gut commensals and their potential as reservoirs of resistance genes. They are also opportunistic pathogens that can cause s...
Contaminated surfaces in food-processing environments represent a significant food safety risk, making the monitoring of hygiene on hands and food-contact surfaces a priority. This study aimed to assess the occurrence and diversity of hygiene indicator bacteria in Portuguese foodservice facilities and to evaluate their association with biocide use. A total of 1,128 hand and surface samples were collected from 1...
The presence of bacterial hazards on contaminated surfaces in food-processing environments poses significant food safety risks. Monitoring and hygiene verification of surfaces in direct or indirect contact with food remains a top priority for food business operators. Sanitation uses antimicrobial compounds, biocides (disinfectants/antiseptics), whose effectiveness on surface microbiota depends on microorganisms...
Antimicrobial resistance (AMR) is a global health threat requiring a One Health approach, asantibiotic-resistant bacteria canspread between animals and humans. Enterococcusspp., particularly E. faecium(Efm) and E. faecalis(Efs),are keyAMR indicators due to their role as gut commensals and their potential as reservoirs of resistance genes.They are also opportunistic pathogensthat can cause severe human infection...
Bacterial hazards on contaminated surfaces in food-processing environments pose significant food safety risks. Hygiene monitoring of surfaces in direct or indirect contact with food typically relies on a limited set of bacterial indicators, such as Enterobacteriaceae counts. However, their genomic diversity remains poorly characterised. The aim of this study was to provide a comprehensive genomic characterisati...
A Segurança dos Alimentos tem impacto direto na proteção dos consumidores e na Saúde Pública, sendo as empresas do setor alimentar os principais responsáveis pela segurança dos géneros alimentícios. Os operadores das empresas de restauração devem garantir a preparação e distribuição de refeições seguras, prevenindo, eliminando ou minimizando os riscos em todas as fases. A contaminação cruzada por microrganismos...
Food safety has a direct impact on consumer protection and public health,with food industry companies being primarily responsible for the safety of food products. Operators of food service businesses must ensure the preparation and distribution of safe meals by preventing, eliminating, or minimising microbiological risks at all stages. Contamination can occur at the final stage of food preparation if proper hyg...
Enterococcus faecium, a human and animal commensal broadly distributed in the environment, is currently one of the most challenging multidrug-resistant (MDR) healthcare-associated pathogens worldwide. It is often exposed to chlorhexidine (CHX), a broad-spectrum antiseptic, extensively used in healthcare, domestic, and food production settings, and a diffused polluter. However, the impact of gradients of CHX con...
Background: Enterococcus faecium-Efm and E.lactis-Elts (former Efm-cladeB) colonize the human gut, with Efm also being a leading hospital-pathogen. Dynamics influencing strain dominance in competitive environments (e.g., infection/colonization) are not fully understood, but bacteriocins may provide competitive advantage to clinical Efm or commensal Elts strains. Objective: We explored bacteriocin content of con...