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Probe-based metagenomic pathogen detection
Ferreira, Rita; Coelho, Luís; Santos, João Dourado; Sobral, Daniel; Isidro, Joana; Mixão, Verónica; Pinto, Miguel; Nunes, Alexandra; Borrego, Maria JoséPublisher Copyright: Copyright © 2025 Ferreira, Coelho, Santos, Sobral, Isidro, Mixão, Pinto, Nunes, Borrego, Lopo, Oleastro, Sousa, Palminha, Veríssimo, Gargaté, Guiomar, Cordeiro, Macedo, Bajanca-Lavado, Paixão, Duarte, Vieira, Borges and Gomes.; Probe-based pathogen enrichment, followed by NGS, is a promising tool for complex diagnosis, overcoming traditional challenges of shotgun metagenomics, namely small ...
Epidemiology and genetic diversity of invasive Neisseria meningitidis strains c...
Bettencourt, Célia; Nunes, Alexandra; Nogueira, Paulo; Duarte, Sílvia; Silva, Catarina; Gomes, João Paulo; Simões, Maria JoãoPublisher Copyright: © 2023, The Author(s).; Invasive meningococcal disease (IMD) continues to be a public health problem due to its epidemic potential, affecting mostly children. We aimed to present a detailed description of the epidemiology of IMD in Portugal, including insights into the genetic diversity of Neisseria meningitidis strains. Epidemiological analysis included data from the Portuguese National Re...
Characterization of the Human Papillomavirus 16 Oncogenes in K14HPV16 Mice
Cochicho, Daniela; Nunes, Alexandra; Gomes, João Paulo; Martins, Luís; Cunha, Mário; Medeiros-Fonseca, Beatriz; Oliveira, Paulaby Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES, Portugal) through national funds to iNOVA4Health (UIDB/04462/2020 and UIDP/ 04462/2020); from the GenomePT project (POCI-01-0145-FEDER-022184), supported by COMPETE 2020—Operational Programme for Competitiveness and Internationalisation (POCI), Lisboa Portugal Regional Operational Programme (Lisboa2020), Alga...
Global gene expression analysis of Streptococcus agalactiae at exponential grow...
Silvestre, Inês; Borges, Vítor; Duarte, Sílvia; Nunes, Alexandra; Sobral, Rita; Vieira, Luís; Gomes, João Paulo; Borrego, Maria JoséA comparative overview of the global gene expression levels of S. agalactiae reference strain NEM316 at the exponential growth phase was done through RNA-sequencing. The expression levels of 47 genes potentially linked to virulence evidenced that: i) the major nuclease, GBS_RS03720/gbs0661, presented higher mean expression values than the remainder of DNase genes; ii) the genetic pilus island PI-2a genes presen...
Genetic Characterization of Brucella spp.
Pelerito, Ana; Nunes, Alexandra; Grilo, Teresa; Isidro, Joana; Silva, Catarina; Ferreira, Ana Cristina; Valdezate, Sylvia; Núncio, Maria SofiaFunding Information: Funding: This work is a result of the GenomePT project (POCI-01- 0145-FEDER-022184), supported by the COMPETE 2020 – Operational Program for Competitiveness and Internationalisation (POCI), Lisboa Portugal Regional Operational Program (Lisboa2020), Algarve Portugal Regional Operational Program (CRESC Algarve2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional ...
Imipenem Resistance in Clostridium difficile Ribotype 017, Portugal
Isidro, Joana; Santos, Andrea; Nunes, Alexandra; Borges, Vitor; Silva, Catarina; Vieira, Luis; Mendes, Aristides L.; Serrano, MonicaWe describe imipenem-resistant and imipenem-susceptible clinical isolates of Clostridium difficile ribotype 017 in Portugal. All ribotype 017 isolates carried an extra penicillin-binding protein gene, pbp5, and the imipenem-resistant isolates had additional substitutions near the transpeptidase active sites of pbp1 and pbp3. These clones could disseminate and contribute to imipenem resistance.
In vitro activity of quaternary ammonium surfactants against streptococcal, chl...
Inácio, Angela S.; Nunes, Alexandra; Milho, Catarina; Mota, Luís Jaime; Borrego, Maria J.; Gomes, João P.; Vaz, Winchil L C; Vieira, Otília V.This work, including the efforts of Otilia V. Vieira, was funded by FCT (PTDC/BIA-BCM/112138/2009). This work, including the efforts of Otilia V. Vieira, was funded by FCT (HMSP-ICT/0024/2010). This work, including the efforts of Otilia V. Vieira, was funded by FCT (iNOVA4Health - UID/Multi/04462/2013).; Quaternary ammonium compounds (QAC) are widely used, cheap, and chemically stable disinfectants and topical ...
Um grande surto da Doença dos Legionários numa cidade industrial em Portugal
George, Francisco; Shivaji, Tara; Pinto, Catia Sousa; Serra, Luis Antonio Oliveira; Valente, João; Albuquerque, Maria JoãoBackground We describe the investigation and control of an outbreak of Legionnaires’ disease in Portugal in October, November and December 2014. Methods Confirmed cases were individuals with pneumonia, laboratory evidence of Legionella pneumophila serogroup 1 and exposure, by residence, occupational or leisure to the affected municipalities. 49 possible sources were reduced to four potential sources, all indust...
Chlamydia Trachomatis in vivo to in vitro transition reveals mechanisms of phas...
Borges, Vítor; Pinheiro, Miguel; Antelo, Minia; Sampaio, Daniel A.; Vieira, Luís; Ferreira, Rita; Nunes, Alexandra; Almeida, Filipe; Mota, Luís J.Fundacao para a Ciencia e a Tecnologia (FCT) (SFRH/BD/68527/2010); Wellcome Trust ISSF grant (097831/Z/11/Z); FCT (SFRH/BD/68532/2010; SFRH/BPD/75295/2010); Research on the obligate intracellular bacterium Chlamydia trachomatis demands culture in cell-lines, but the adaptive process behind the in vivo to in vitro transition is not understood. We assessed the genomic and transcriptomic dynamics underlying C.Trac...