89 documents found, page 1 of 9
Implementação de um questionário epidemiológico a detentores de explorações cas...
Esteves, Ana; Aniceto, Carlos; Baroni, Bruno; Ramos, Sónia; Ferreira, Rita; Gomes, João Paulo; Machado, Ausenda; Nunes, AlexandraA detenção e a criação de animais em explorações caseiras podem representar um risco para a transmissão de agentes patogénicos zoonóticos e de genes de resistência a antibióticos, com impacto na saúde pública. Em Portugal, a ausência de registo de sistemas de produção caseira (SPC) dificulta a caracterização das práticas de gestão e criação de animais, assim como o perfil dos seus proprietários. Este estudo pil...
Genomic Analysis of Antibiotic Resistance and Virulence Profiles in Escherichia...
Ribeiro, Jessica; Silva, Vanessa; Freitas, Catarina; Pinto, Pedro; Vieira-Pinto, Madalena; Batista, Rita; Nunes, Alexandra; Gomes, João PauloSternal bursitis is an underexplored lesion in poultry, often overlooked in microbiological diagnostics. In this study, we characterized 36 Escherichia coli isolates recovered from sternal bursitis in broiler chickens, combining phenotypic antimicrobial susceptibility testing, PCR-based screening, and whole genome sequencing (WGS). The genetic analysis revealed a diverse population spanning 15 sequence types, i...
Distribution of Chlamydia trachomatis ompA-genotypes over three decades in Port...
Lodhia, Zohra; Cordeiro, Dora; Correia, Cristina; João, Inês; Carreira, Teresa; Vieira, Luís; Nunes, Alexandra; Ferreira, Rita; Schäfer, SandraObjectives: Chlamydia trachomatis is classified into 15 major genotypes, A to L3, based on the diversity of ompA gene. Here, we evaluated and characterised the distribution and diversity of ompA-genotypes over 32 years (1990–2021) in Portugal. Methods: The collection of the Portuguese National Reference Laboratory for Sexually Transmitted Infections includes 5824 C. trachomatis-positive samples that were succes...
Lymphogranuloma venereum (LGV) ompA-subvariants of the Portuguese collection of...
Lodhia, Zohra; Cordeiro, Dora; Correia, Cristina; João, Inês; Carreira, Teresa; Nunes, Alexandra; Ferreira, Rita; Schäfer, Sandra; Aliyeva, ElzaraBackground: Lymphogranuloma venereum (LGV) is a sexually transmitted infection caused by Chlamydia trachomatis ompA-genotypes L1–L3, with increasing numbers of detected cases across Europe. Here, we analysed diversity and temporal distribution of the LGV ompA-subvariants detected in Portugal between 2007 and 2023, in order to better understand the dissemination and diversification landscape of LGV strains. Meth...
Probe-based metagenomic pathogen detection: advancing laboratory capacity for c...
Ferreira, Rita; Coelho, Luís; Santos, João Dourado; Sobral, Daniel; Isidro, Joana; Mixão, Verónica; Pinto, Miguel; Nunes, Alexandra; Borrego, Maria JoséProbe-based pathogen enrichment, followed by NGS, is a promising tool for complex diagnosis, overcoming traditional challenges of shotgun metagenomics, namely small microbial/human genetic material ratio and demanding computational resources. Here, we assessed the combined detection performance of two Illumina probe-based panels, the Respiratory and the Urinary Pathogen ID panels (RPIP and UPIP), using 99 clini...
Phenotypic and Genotypic Characterization of Escherichia coli and Salmonella sp...
Gonçalves, Carlota; Silveira, Leonor; Rodrigues, João; Furtado, Rosália; Ramos, Sónia; Nunes, Alexandra; Pista, ÂngelaBackground: Foodborne diseases are a serious public health concern, and food-producing animals are a major source of contamination. Methods: The present study analysed Escherichia coli and Salmonella spp. isolated from faecal samples of 100 fattening pigs and from 52 samples of pork meat. Results: The results showed that the majority of the analysed meat samples were considered satisfactory in terms of microbio...
Preclinical assessment of an antibiotic-free cationic surfactant-based cellulos...
Calado, Rita D.A.; Mendes, Bárbara B.; Conniot, João; Ravasco, João M.J.M.; Sobral, Daniel; Ferreira, Carolina; Ferreira, Rita; Rodrigues, João CarlosSexually transmitted infections and urogenital-perinatal infections are significant health challenges owing to their asymptomatic nature, multidrug-resistant pathogens, and lack of effective vaccines. Surfactants are under investigation as potential antimicrobial agents and alternatives to traditional antibiotics. Here, we discovered that N-dodecylpyridinium bromide (C12PB), a cationic quaternary ammonium surfa...
Epidemiology of invasive meningococcal disease in Portugal from 2012 to 2022
Bettencourt, Célia; Nunes, Alexandra; VigLab-DM – Network for the Laboratory Surveillance of Meningococcal Disease; Simões, Maria JoãoBackroud and aim: Surveillance of invasive meningococcal disease (IMD) is essential to monitor changes in the epidemiology of the disease and for more effective infection control. In Portugal, a surveillance system based on mandatory clinical and laboratory notifications was implemented in late 2002, with the laboratory component under the responsibility of the National Reference Laboratory (NRL) for Neisseria ...
Epidemiology and genetic diversity of invasive Neisseria meningitidis strains c...
Bettencourt, Célia; Nunes, Alexandra; Nogueira, Paulo; Duarte, Sílvia; Silva, Catarina; Gomes, João Paulo; Simões, Maria JoãoInvasive meningococcal disease (IMD) continues to be a public health problem due to its epidemic potential, affecting mostly children. We aimed to present a detailed description of the epidemiology of IMD in Portugal, including insights into the genetic diversity of Neisseria meningitidis strains. Epidemiological analysis included data from the Portuguese National Reference Laboratory of Neisseria meningitidis ...
Viral genetic clustering and transmission dynamics of the 2022 mpox outbreak in...
Borges, Vítor; Duque, Mariana Perez; Martins, João Vieira; Vasconcelos, Paula; Ferreira, Rita; Sobral, Daniel; Pelerito, Ana; de Carvalho, Isabel LopesPathogen genome sequencing during epidemics enhances our ability to identify and understand suspected clusters and investigate their relationships. Here, we combine genomic and epidemiological data of the 2022 mpox outbreak to better understand early viral spread, diversification and transmission dynamics. By sequencing 52% of the confirmed cases in Portugal, we identified the mpox virus sublineages with the hi...