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Seasonal biochemical fingerprints of outdoor cultivated Spirulina (Limnospira p...

Lopes, Diana; Moreira, Ana S. P.; Conde, Tiago; Ferreira, Andreia; Oliveira, Kayane; Conde, Alexandra; Ramos, Ana Alexandra; Pereira, Hugo

Microalgae are increasingly recognized as sustainable bioresources with applications in food, feed, nutraceuticals, and aquaculture. Among them, Spirulina (Limnospira platensis) and Microchloropsis gaditana (formerly Nannochloropsis gaditana) stand out due to their rich biochemical composition with industrial applications and high market value. This study separately investigates the seasonal variability in the ...


Boosting through-plane electrical conductivity: chitosan composite films with c...

Barra, Ana; Ferreira, Nuno M.; Pocas, Fátima; Ruiz-Hitzky, Eduardo; Nunes, Cláudia; Ferreira, Paula

Flexible and electrically conductive materials are gaining significant attention across various domains, notably in electronics, biomedicine and food industry. One promising strategy involves the integration of electrically conductive nanostructures into a polymeric matrix to fabricate composite materials. However, achieving uniform through-plane electrical conductivity remains a challenge due to the preferenti...


Unlocking biochemical profile of chaetoceros calcitrans, cylindrotheca fusiform...

Moreira, Ana S. P.; Rocha, Helena R.; Aveiro, Susana; Ferreira, Andreia S.; Oliveira, Kayane; Conde, Alexandra; Coelho, Marta

Diatoms have garnered attention as sources of natural bioactive compounds, making them attractive for developing high-value products for different biotechnological ends, particularly for nutritional applications. Despite their potential, the chemical composition of industrially produced biomass remains largely underexplored. In this context, this study was focused on a thorough characterisation of the biochemic...


Defatted nannochloropsis oculata biomass: waste or resource?

Sousa, Sérgio; Machado, Manuela; Coscueta, Ezequiel; Ferreira, Andreia S.; Nunes, Cláudia; Coimbra, Manuel A.; Freitas, Ana C.; Carvalho, Ana P.

Microalgae are recognized as a valuable source of a panoply of compounds. In addition to the extensively investigated lipid fraction comprising polyunsaturated fatty acids (PUFA), microalgae biomass also encompasses other compounds with potentially relevant biological activities. This work innovatively repurposes the defatted biomass (DB) of Nannochloropsis oculata, highlighting its potential value beyond the l...


Water-Soluble Saccharina latissima Polysaccharides and Relation of Their Struct...

Moreira, Ana S. P.; Gaspar, Diana; Ferreira, Sónia S.; Correia, Alexandra; Vilanova, Manuel João Rua; Perrineau, Marie-Mathilde; Kerrison, Philip D.

Brown macroalgae are an important source of polysaccharides, mainly fucose-containing sulphated polysaccharides (FCSPs), associated with several biological activities. However, the structural diversity and structure-function relationships for their bioactivities are still undisclosed. Thus, the aim of this work was to characterize the chemical structure of water-soluble Saccharina latissima polysaccharides and ...


Contribution of non-ionic interactions on bile salt sequestration by chitooligo...

Coreta-Gomes, Filipe Manuel; Silva, Inês M. V.; Nunes, Cláudia; Marin-Montesinos, Ildefonso; Evtuguin, Dmitry Victorovitch; Geraldes, Carlos F. G. C.

Chitooligosaccharides have been suggested as cholesterol reducing ingredients mostly due to their ability to sequestrate bile salts. The nature of the chitooligosaccharides-bile salts binding is usually linked with the ionic interaction. However, at physiological intestinal pH range (6.4 to 7.4) and considering chitooligosaccharides pKa, they should be mostly uncharged. This highlights that other type of intera...


Stabilization of natural pigments in ethanolic solutions for food applications:...

Ferreira, Andreia S.; Pereira, Liliana; Canfora, Feliciana; Silva, Tiago H.; Coimbra, Manuel A.; Nunes, Cláudia

<i>Chlorella vulgaris</i> is a green microalga with a high chlorophyll content, representing a valuable source of green pigments for food applications. As the application of whole biomass can promote an unpleasant fish-like flavor, the use of chlorophyll extract can overcome this drawback. However, chlorophylls tend to easily degrade when out of the chloroplasts, decreasing their potential as a food ingredient....


Fucoidan from Fucus vesiculosus inhibits inflammatory response, both in vitro a...

Wang, Lingzhi; Oliveira, Ana Catarina Freitas Salazar; Li, Qiu; Ferreira, Andreia S.; Nunes, Cláudia; Coimbra, Manuel A.; Reis, R. L.; Martins, Albino

Fucoidan has been reported to present diverse bioactivities, but each extract has specific features from which a particular biological activity, such as immunomodulation, must be confirmed. In this study a commercially available pharmaceutical-grade fucoidan extracted from Fucus vesiculosus, FE, was characterized and its anti-inflammatory potential was investigated. Fucose was the main monosaccharide (90 mol%) ...


Chemical composition and antioxidant potential of five algae cultivated in full...

Nova, Paulo; Pimenta-Martins, Ana; Maricato, Élia; Nunes, Cláudia; Abreu, Helena; Coimbra, Manuel A.; Freitas, Ana Cristina; Gomes, Ana Maria

In this study, the chemical composition and antioxidant profile of five edible macroalgae, Fucus vesiculosus, Palmaria palmata, Porphyra dioica, Ulva rigida, and Gracilaria gracilis, cultivated in fully controlled closed systems, were determined. Protein, carbohydrates, and fat contents ranged between 12.4% and 41.8%, 27.6% and 42.0%, and 0.1% and 3.4%, respectively. The tested seaweeds presented considerable a...


Potential of Coccolithophore microalgae as fillers in starch-based films for ac...

Moreira, Ana S. P.; Gonçalves, Joana; Sousa, Francisco; Maia, Inês Beatriz; Pereira, Hugo; Silva, Joana; Coimbra, Manuel A.; Ferreira, Paula

Coccolithophore microalgae, such as Emiliania huxleyi (EHUX) and Chrysotila pseudoroscoffensis (CP), are composed of calcium carbonate (CaCO3) and contain bioactive compounds that can be explored to produce sustainable food packaging. In this study, for the first time, these microalgae were incorporated as fillers in starch-based films, envisioning the development of biodegradable and bioactive materials for fo...


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