20 documents found, page 1 of 2
Development of a canine epidermis equivalent model for evaluation of sensitization
Marques, M; Nunes, J; Ustymenko, B; Lagoa, Tânia; Fialho, Luísa; Martins, Luís; Burke, Anthony; Souza, E; Filho, César; Craveiro, Alexandre; Costa, AnaBackground Canine atopic dermatitis (cAD) is a genetic-predisposed allergic and pruritic inflammatory skin condition, associated with sensitization to environmental allergens. Keratinocytes can produce several inflammatory mediators, in response to several other host mediators, antigens, and pathogens by virtue of their wide range of surface receptors, some of them dysregulated in cAD individuals. A histotypica...
Development of a canine skin analog
Marques, M; Nunes, J; Fialho, Luisa; Martins, Luís; Burke, Anthony; Souza, E; Craveiro, Alexandre; Costa, Ana; Branco, Sandra; Antunes, CéliaBackground. Skin covers an extensive area of the body and comprises three major layers, subcutis, dermis, and epidermis. This organ is one of the most tested for toxicity and safety evaluation during the process of drug research and development, in past usually performed in vivo using animals. On the last years a concern of sustainable and eco-friendly approach has been developing, with the expansion of non-ani...
Biocompatibility and biosafety analysis of chitosan hydrogels using organotypic...
Nunes, J; Marques, M; Sterghite, D; Branco, S; Martins, Luís; Silva, M; Campos-Gonçalves, I; Souza, E; Filho, César; Craveiro, AlexandreBackground: Frequent dog consultations are due to skin wounds (Holland, 2019), with different trauma causes, like bites, burns and others. The majority of these wounds are not fatal, but cause long-lasting discomfort, demanding frequent medical care which may affect owners financially (Fahie & Shettko, 2007). Despite several pharmaceuticals available for dog wound treatment, new antibiotic-free efficient option...
Immune and Spermatogenesis-Related Loci are Involved in the Development of Extr...
Cerván-Martín, M; Tüttelmann, F; Lopes, AM; Bossini-Castillo, L; Rivera-Egea, R; Garrido, N; Lujan, S; Romeu, G; Santos-Ribeiro, S; Castilla, JAWe conducted a genome-wide association study in a large population of infertile men due to unexplained spermatogenic failure (SPGF). More than seven million genetic variants were analysed in 1,274 SPGF cases and 1,951 unaffected controls from two independent European cohorts. Two genomic regions were associated with the most severe histological pattern of SPGF, defined by Sertoli cell-only (SCO) phenotype, name...
Fabry Disease Caused by the GLA p.Phe113Leu (p.F113L) Variant: Natural History ...
Oliveira, J; Nowak, A; Barbey, F; Torres, M; Nunes, J; Teixeira-e-Costa, F; Carvalho, F; Sampaio, S; Tavares, I; Pereira, O; Soares, A; Carmona, CBackground, aims and methods: The α-galactosidase gene (GLA) c.337T>C/p.Phe113Leu variant was originally described in patients with late-onset cardiac forms of Fabry disease (FD), who had residual α-galactosidase activity. It has since emerged as the most commonly reported GLA variant in Portuguese subjects diagnosed with FD but is also prevalent in the Italian population, where two boys carrying the GLA Leu113...
Evaluation of the role of mitochondria in the non-targeted effects of ionizing ...
Miranda, S; Correia, M; Dias, AG; Pestana, A; Soares, P; Nunes, J; Lima, J; Máximo, V; Boaventura, PRadiobiology is moving towards a better understanding of the intercellular signaling that occurs upon radiation and how its effects relate to the dose applied. The mitochondrial role in orchestrating this biological response needs to be further explored. Cybrids (cytoplasmic hybrids) are useful cell models for studying the involvement of mitochondria in cellular processes. In the present study we used cybrid ce...
Association of Pubertal Development With Adiposity and Cardiometabolic Health i...
Fonseca, MJ; Oliveira, A; Azevedo, I; Nunes, J; Santos, ACPurpose Early timing of pubertal development is associated with worse cardiometabolic health in adulthood. We aimed to evaluate this association in 10-year-old girls and boys and clarify if it is independent of previous body mass index (BMI). Methods Pubertal development was evaluated through the Tanner scale in 4,548 children from the birth cohort Generation XXI. Data on anthropometrics, body composition, bloo...
High resolution mass spectrometry-based methodologies for identification of Etr...
Godinho, A; Martins, I; Nunes, J; Charneira, C; Grilo, J; Silva, D; Pereira, S; Soto, K, et al.Drug bioactivation to reactive metabolites capable of covalent adduct formation with bionucleophiles is a major cause of drug-induced adverse reactions. Therefore, elucidation of reactive metabolites is essential to unravel the toxicity mechanisms induced by drugs and thereby identify patient subgroups at higher risk. Etravirine (ETR) was the first second-generation Non-Nucleoside Reverse Transcriptase Inhibito...
Alentejano and Bísaro pigs: Tradition and innovation – The TREASURE Project
Charneca, R; Freitas, A; Martins, J; Neves, J; Elias, M; Laranjo, M; Nunes, JThe Alentejano pig (AL) and the Bísaro pig (BI) are the two main autochthonous swine breeds of Portugal. Originated from different ancestors they have a similar history across the last century. One hundred years ago AL was the most common swine breed in Portugal, but in the second half of the 20th century both Portuguese breeds undertook a strong population decline that almost led to their extinction. However, ...
Acetabular retroversion: Diagnosis and treatment
Direito-Santos, B; França, G; Nunes, J; Costa, A; Rodrigues, EB; Silva, AP; Varanda, PAcetabular retroversion (AR) consists of a malorientation of the acetabulum in the sagittal plane. AR is associated with changes in load transmission across the hip, being a risk factor for early osteoarthrosis. The pathophysiological basis of AR is an anterior acetabular hyper-coverage and an overall pelvic rotation.The delay or the non-diagnosis of AR could have an impact in the overall management of femoroac...