12 documents found, page 1 of 2
Host soluble factors cause changes in Staphylococcus epidermidis antibiotic sus...
Oliveira, Fernando Eduardo Freitas; Gaio, Vânia Silva; Brás, Susana Maria Pereira; Oliveira, Sofia; França, Ângela Maria Oliveira SousaStaphylococcus epidermidis is a major nosocomial pathogen with a remarkable ability to adhere to the surfaces of indwelling medical devices and form biofilms. Unlike other nosocomial pathogens, the interaction of S. epidermidis with host factors has not been the focus of substantial research. This study aimed to assess the alterations in the antibiotic susceptibility and biofilm formation ability of S. epidermi...
mazEF homologue has a minor role in Staphylococcus epidermidis 1457 virulence p...
Gaio, Vânia Silva; Lima, Tânia; Vilanova, Manuel; Cerca, Nuno; França, Ângela Maria Oliveira SousaThe Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2021.803134/ full#supplementary-material; Staphylococcus epidermidis biofilm cells are characterized by increased antimicrobial tolerance and improved ability to evade host immune system defenses. These features are, in part, due to the presence of viable but non-culturable (VBNC) cells. A prev...
S. epidermidis putative toxin-antitoxin module mazEF: the role in VBNC formatio...
Gaio, Vânia SilvaTese de doutoramento em Engenharia Biomédica; Staphylococcus epidermidis é um microrganismo comensal que habita na pele e membranas mucosas dos seres humanos e outros mamíferos, mas que pode atuar como um agente patogénico oportunista, especialmente em pacientes imunocomprometidos e em bebés prematuros. Os principais fatores de virulência de S. epidermidis são a sua reduzida suscetibilidade a antibióticos e a s...
Thymbra capitata essential oil has a significant antimicrobial activity against...
Almeida, Luciana; Lopes, Nathalie; Gaio, Vânia Silva; Cavaleiro, Carlos; Salgueiro, Lígia; Silva, Vanessa; Poeta, Patrícia; Cerca, NunoAbstract Methicillin-resistant Staphylococcus aureus (MRSA) is a multidrug-resistant opportunistic pathogen with a great ability to form biofilms. Herein, the antimicrobial potential of Thymbra capitata essential oil (EO) against MRSA biofilms was investigated. The determination of the minimum inhibitory concentration (MIC) and the minimum lethal concentration (MLC) of the T. capitata EO was first investigated ...
Virulence factors in coagulase-negative staphylococci
França, Ângela Maria Oliveira Sousa; Gaio, Vânia Silva; Lopes, Nathalie; Melo, Luís Daniel RodriguesCoagulase-negative staphylococci (CoNS) have emerged as major pathogens in healthcare-associated facilities, being S. epidermidis, S. haemolyticus and, more recently, S. lugdunensis, the most clinically relevant species. Despite being less virulent than the well-studied pathogen S. aureus, the number of CoNS strains sequenced is constantly increasing and, with that, the number of virulence factors identified in...
Biofilm formation of multidrug-resistant MRSA strains isolated from different t...
Silva, Vanessa; Almeida, Luciana Mayara Mendonça; Gaio, Vânia Silva; Cerca, Nuno; Manageiro, Vera; Caniça, Manuela; Capelo, José L.; Igrejas, GilbertoMethicillin-resistant Staphylococcus aureus (MRSA) is one of the main pathogens causing chronic infections, mainly due to its capacity to form biofilms. However, the mechanisms underlying the biofilm formation of MRSA strains from different types of human infections are not fully understood. MRSA strains isolated from distinct human infections were characterized aiming to determine their biofilm-forming capacit...
The putative toxin-antitoxin mazEF gene cluster is not involved in cell death i...
Gaio, Vânia Silva; Cerca, Nuno; França, Ângela Maria Oliveira SousaBackground: Toxin-antitoxin (TA) systems are known to work as a poison-antidote system, where the action of the antitoxin neutralizes the effect of the toxin counterpart. mazEF has been identified as a TA system in several species and is associated with the bacterial stress response. A previous study showed that the formation of viable but non-culturable (VBNC) cells in S. epidermidis biofilms might be linked t...
Biofilm released cells can easily be obtained in a fed-batch system using ica+ ...
Gaio, Vânia Silva; Cerca, NunoSupplemental information for this article can be found online at http://dx.doi.org/10.7717/peerj.9549#supplemental-information.; Staphylococcus epidermidis is one of the major opportunistic bacterial pathogens in healthcare facilities, mainly due to its strong ability to form biofilms in the surface of indwelling medical devices. To study biofilms under in vitro conditions, both fed-batch and flow systems are w...
Cells released from S. epidermidis biofilms present increased antibiotic tolera...
Gaio, Vânia Silva; Cerca, NunoBiofilm released cells (Brc) are thought to present an intermediary phenotype between biofilm and planktonic cells and this has the potential of affecting their antimicrobial tolerance. Aim Compare the antimicrobial tolerance profiles of Brc, planktonic or biofilm cultures of S. epidermidis. Methodology Planktonic, biofilm cultures or Brc from 11 isolates were exposed to peak serum concentrations (PSC) of antib...
Does mazEF have a role in S. epidermidis biofilm dormancy?
Gaio, Vânia Silva; França, Ângela Maria Oliveira Sousa; Cerca, NunoIntroduction: S. epidermidis is one of the main causes of nosocomial infections associated with the use of medical devices, due to its ubiquitous presence in human skin and mucosae and capacity to form biofilms. Biofilms are a major concern in healthcare systems, since they present higher antimicrobial tolerance and ability to evade host immune defenses, leading to recurrent and relapsing infections. Moreover, ...