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Direct PCR detection of foot-and-mouth disease virus
Rodriguez, A.; Nunez, J.; Nolasco, Gustavo; Ponz, F.; Sobrino, F.; Deblas, C.A PCR assay for the detection and characterization of foot-and-mouth disease virus was developed. The procedure allows RT-PCR amplification following direct adsorption of viral suspensions to microtiter plates, avoiding previous steps of phenol-extraction or heating. Using this procedure, FMDV-specific (based on 3D gene sequences), as well as serotype-specific (based on VP1 gene sequences) amplification were ac...
Date: 1994
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Origin: Sapientia - Universidade do Algarve