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Density separation of petrous bone powders for optimized ancient DNA yields

Fernandes, Daniel; Sirak, Kendra A.; Cheronet, Olivia; Novak, Mario; Brück, Florian; Zelger, Evelyn; Llanos-Lizcano, Alejandro; Wagner, Anna

Density separation is a process routinely used to segregate minerals, organic matter, and even microplastics, from soils and sediments. Here we apply density separation to archaeological bone powders before DNA extraction to increase endogenous DNA recovery relative to a standard control extraction of the same powders. Using nontoxic heavy liquid solutions, we separated powders from the petrous bones of 10 indi...


A genetic history of continuity and mobility in the Iron Age central Mediterranean

Moots, Hannah M.; Antonio, Margaret; Sawyer, Susanna; Spence, Jeffrey P.; Oberreiter, Victoria; Weiß, Clemens L.; Lucci, Michaela

The Iron Age was a dynamic period in central Mediterranean history, with the expansion of Greek and Phoenician colonies and the growth of Carthage into the dominant maritime power of the Mediterranean. These events were facilitated by the ease of long-distance travel following major advances in seafaring. We know from the archaeological record that trade goods and materials were moving across great distances in...


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